占智高;关丽梅;况文东;王金昌;陈俊晖;周溪;范兆军;邓菲;王君;靳亮;

• 1:江西省科学院微生物研究所
• 2:中国科学院武汉病毒研究所

摘要(Abstract)：

从自然死亡的雀纹天蛾幼虫分离到一株雀纹天蛾核型多角体病毒。通过扫描及切片透射电镜发现，该病毒为单粒包埋型核型多角体病毒，命名为ThjaSNPV(Theretra japonica single nucleopolyhedrovirus)。病毒全基因组重测序后拼接显示，该病毒基因组全长134 899 bp,GC含量37.28%，与豆天蛾单粒包埋型核型多角体病毒株DZ1基因组序列相似性高达96.24%。ThjaSNPV含有131个开放阅读框（ORF）其中55个为正链基因，76个为负链基因，与宿主同为天蛾科的豆天蛾单粒包埋型核型多角体基因组相比，雀纹天蛾单粒包埋型核型多角体病毒新注释到7个基因：chitin-binding protein(ORF9),ODV-E18(ORF11),lef-11(ORF27),hypothetical protein(ORF41),lef-10(ORF42),pif-6(ORF66),P6.9(ORF88)。ThjaSNPV的DNA光裂酶基因（DNA photolyase,ORF53）中不具有豆天蛾NPV中的1bp碱基的缺失，只编码一个大的完整DNA裂合酶。38个串联的核心基因的进化分析显示雀纹天蛾NPV与Alphabaculovirus group II类群聚类在一起，且和豆天蛾NPV最为相似。

关键词(KeyWords)： 雀纹天蛾单粒包埋型核型多角体病毒;基因组;DNA光裂酶;序列分析

Abstract:

Keywords:

基金项目(Foundation): 江西省自然科学基金资助项目（项目号：20192ACB20008），题目：夹竹桃天蛾质型多角体病毒VP5蛋白参与病毒复制的功能研究;; 江西省重点研发项目（项目号：20192BBF60056），题目：草原毛虫多角体病毒复配增效杀虫剂关键技术及应用研究;江西省重点研发项目（项目号：20202BBFL63050），题目：扁刺蛾核型多角体病毒杀虫剂生产关键技术;江西省重点研发项目（项目号：20212BBF63044），题目：豆天蛾质型多角体病毒杀虫剂关键技术研究~~

作者(Authors): 占智高;关丽梅;况文东;王金昌;陈俊晖;周溪;范兆军;邓菲;王君;靳亮;

DOI: 10.13242/j.cnki.bingduxuebao.004132

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