一株雀纹天蛾核型多角体病毒的鉴定及其基因组序列分析Characterization and Comparative Genomic Analysis of the Single Nucleopolyhedrovirus of Theretra Japonica
占智高;关丽梅;况文东;王金昌;陈俊晖;周溪;范兆军;邓菲;王君;靳亮;
摘要(Abstract):
从自然死亡的雀纹天蛾幼虫分离到一株雀纹天蛾核型多角体病毒。通过扫描及切片透射电镜发现,该病毒为单粒包埋型核型多角体病毒,命名为ThjaSNPV(Theretra japonica single nucleopolyhedrovirus)。病毒全基因组重测序后拼接显示,该病毒基因组全长134 899 bp,GC含量37.28%,与豆天蛾单粒包埋型核型多角体病毒株DZ1基因组序列相似性高达96.24%。ThjaSNPV含有131个开放阅读框(ORF)其中55个为正链基因,76个为负链基因,与宿主同为天蛾科的豆天蛾单粒包埋型核型多角体基因组相比,雀纹天蛾单粒包埋型核型多角体病毒新注释到7个基因:chitin-binding protein(ORF9),ODV-E18(ORF11),lef-11(ORF27),hypothetical protein(ORF41),lef-10(ORF42),pif-6(ORF66),P6.9(ORF88)。ThjaSNPV的DNA光裂酶基因(DNA photolyase,ORF53)中不具有豆天蛾NPV中的1bp碱基的缺失,只编码一个大的完整DNA裂合酶。38个串联的核心基因的进化分析显示雀纹天蛾NPV与Alphabaculovirus group II类群聚类在一起,且和豆天蛾NPV最为相似。
关键词(KeyWords): 雀纹天蛾单粒包埋型核型多角体病毒;基因组;DNA光裂酶;序列分析
基金项目(Foundation): 江西省自然科学基金资助项目(项目号:20192ACB20008),题目:夹竹桃天蛾质型多角体病毒VP5蛋白参与病毒复制的功能研究;; 江西省重点研发项目(项目号:20192BBF60056),题目:草原毛虫多角体病毒复配增效杀虫剂关键技术及应用研究;江西省重点研发项目(项目号:20202BBFL63050),题目:扁刺蛾核型多角体病毒杀虫剂生产关键技术;江西省重点研发项目(项目号:20212BBF63044),题目:豆天蛾质型多角体病毒杀虫剂关键技术研究~~
作者(Authors): 占智高;关丽梅;况文东;王金昌;陈俊晖;周溪;范兆军;邓菲;王君;靳亮;
DOI: 10.13242/j.cnki.bingduxuebao.004132
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