秦萌;王大燕;黄芳;聂凯;曲梅;王淼;韩峰;赵翔;成艳辉;舒跃龙;马学军;

• 1:中国疾病预防控制中心病毒病预防控制所病毒基因工程国家重点实验室
• 2:北京市疾病预防控制中心传染病地方病控制所

摘要(Abstract)：

本研究设计的一种4重荧光定量RT-PCR检测方法,以A型流感病毒各亚型的血凝素基因(HA)为检测靶标,实现了同时检测新甲型H1N1流感病毒、人季节性H1N1流感病毒和人季节性H3N2流感病毒。本法使用人细胞RNA酶P基因作为内参,以判断标本来源和实施质量控制。利用不同来源和亚型的流感病毒验证了该方法的特异性;利用连续稀释的新甲型H1N1流感病毒HA全基因体外转录物进行灵敏度分析。结果表明该方法灵敏度高,可检测低至20个拷贝的RNA;特异性强,每对引物只检测出对应的病毒,无交叉反应;并且成功地验证性检验了34份新甲型H1N1流感病毒阳性临床标本和20份人季节性H1N1和H3N2流感病毒及人乙型(HB)流感病毒阳性临床标本。因此,该多重荧光定量RT-PCR法是一种可同时检测2009年新甲型流感病毒及季节性流感病毒的有效方法。

关键词(KeyWords)： 新甲型H1N1;实时定量PCR;多重检测

Abstract:

Keywords:

基金项目(Foundation): 传染病防治重大专项(2009ZX10004-101,2008ZX10004-002,2008ZX10004-001,2008ZX10004-004)

作者(Authors): 秦萌;王大燕;黄芳;聂凯;曲梅;王淼;韩峰;赵翔;成艳辉;舒跃龙;马学军;

DOI: 10.13242/j.cnki.bingduxuebao.002069

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