张金城;马德青;徐贝贝;冯伟民;陈鑫;韦天超;韦平;黄腾;

• 1:广西大学动物科学技术学院

摘要(Abstract)：

马立克病病毒（Marek's disease virus,MDV）UL56基因编码α-疱疹病毒同源蛋白pUL56。为全面了解该病毒蛋白与宿主细胞的互作关系，本研究首先将表达红色荧光蛋白标记的MDV pUL56质粒转染鸡DF-1细胞系，瞬时表达蛋白的细胞经流式细胞术分选，富集阳性细胞群进行高通量测序及mRNA转录组分析。测序结果显示，表达pUL56的DF-1细胞存在914个差异表达基因，其中462个上调，452个下调。通过GO、KEGG和Reactome通路富集分析，发现富集的显著差异表达基因分布于先天性免疫反应、细胞因子产生等相关信号通路。在此基础上，随机选取了I型干扰素信号通路和促炎症细胞因子基因进行RT-qPCR验证，IFI6、IFIH1、CD83、HSP90AA1、IL-1β和IL-8L1基因的表达量均为上调，而IL-12β表达则下调，该结果与高通量测序相符。本研究提示pUL56作为非结构蛋白，可能对宿主细胞过程有广泛的调节作用。

关键词(KeyWords)： 马立克病病毒;pUL56;转录组;信号通路;差异表达基因

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金项目(项目号：31702236)，题目：马立克氏病病毒跨膜蛋白干扰MHC-I抗原呈递的分子机制研究~~

作者(Authors): 张金城;马德青;徐贝贝;冯伟民;陈鑫;韦天超;韦平;黄腾;

DOI: 10.13242/j.cnki.bingduxuebao.004147

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