马立克病病毒pUL56蛋白对DF-1细胞免疫通路相关基因的转录调控研究Transcriptomic Profiling of DF-1 Cells with Transient Expression of the pUL56Protein of the Marek's Disease Virus
张金城;马德青;徐贝贝;冯伟民;陈鑫;韦天超;韦平;黄腾;
摘要(Abstract):
马立克病病毒(Marek's disease virus,MDV)UL56基因编码α-疱疹病毒同源蛋白pUL56。为全面了解该病毒蛋白与宿主细胞的互作关系,本研究首先将表达红色荧光蛋白标记的MDV pUL56质粒转染鸡DF-1细胞系,瞬时表达蛋白的细胞经流式细胞术分选,富集阳性细胞群进行高通量测序及mRNA转录组分析。测序结果显示,表达pUL56的DF-1细胞存在914个差异表达基因,其中462个上调,452个下调。通过GO、KEGG和Reactome通路富集分析,发现富集的显著差异表达基因分布于先天性免疫反应、细胞因子产生等相关信号通路。在此基础上,随机选取了I型干扰素信号通路和促炎症细胞因子基因进行RT-qPCR验证,IFI6、IFIH1、CD83、HSP90AA1、IL-1β和IL-8L1基因的表达量均为上调,而IL-12β表达则下调,该结果与高通量测序相符。本研究提示pUL56作为非结构蛋白,可能对宿主细胞过程有广泛的调节作用。
关键词(KeyWords): 马立克病病毒;pUL56;转录组;信号通路;差异表达基因
基金项目(Foundation): 国家自然科学基金项目(项目号:31702236),题目:马立克氏病病毒跨膜蛋白干扰MHC-I抗原呈递的分子机制研究~~
作者(Authors): 张金城;马德青;徐贝贝;冯伟民;陈鑫;韦天超;韦平;黄腾;
DOI: 10.13242/j.cnki.bingduxuebao.004147
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