冯兆民;舒跃龙;

• 1:中国疾病预防控制中心病毒病预防控制所世界卫生组织全球流感参比和研究合作中心感染性疾病诊治协同创新中心卫生和计划生育委员会医学病毒和病毒病重点实验室

摘要(Abstract)：

数字PCR(Digital PCR,dPCR)是核酸绝对定量的新方法,该技术通过分液,将含有核酸模板的PCR反应体系分配到上万个反应器中进行PCR扩增,根据荧光信号的有或无,进行结果计数,通过泊松分布的统计处理,直接得出核酸的拷贝数。相比于实时荧光定量PCR(Quantitative PCR,qPCR),dPCR不需要建立标准曲线,应用前景更广。本文对dPCR的发展历史、原理及其应用进行了综述。

关键词(KeyWords)： 数字PCR;绝对定量;拷贝数;实时荧光定量PCR

Abstract:

Keywords:

基金项目(Foundation): 北京市科技计划项目(项目号:D151100002115003);题目:各级综合医院病毒性肺炎筛查检测技术研究;; 国家科技支撑计划(项目号:2015BAI12B11);题目:病毒性肺炎诊治和网络建设

作者(Authors): 冯兆民;舒跃龙;

DOI: 10.13242/j.cnki.bingduxuebao.003088

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