王慧玲;郑蕾;王骥涛;郜慧;张燕;孔晓慧;许文波;

• 1:首都医科大学附属北京儿童医院
• 2:中国疾病预防控制中心病毒病预防控制所
• 3:山西省疾病预防控制中心
• 4:太原市疾病预防控制中心

摘要(Abstract)：

本文报道了2009年中国首例输入性D4基因型麻疹病例。使用Vero/SLAM细胞(淋巴信号激活因子转染的非洲绿猴肾细胞)对该麻疹病例的咽拭子标本进行麻疹病毒分离,经逆转录聚合酶链反应(Reverse transcript polymerase chain reaction,RT-PCR)方法扩增出麻疹病毒核蛋白(Nucleoprotein,N)羧基(COOH)端676个核苷酸片段。通过对扩增产物进行核苷酸序列测定和分析,以COOH端450个核苷酸片段构建基因亲缘性关系树,进行核苷酸和氨基酸同源性分析。该麻疹病毒分离株(MVi/Shanxi.CHN/20.09/1)和WHOD4基因型参考株Mont-real.CAN/89在基因亲缘性关系树上同属一个分支,核苷酸同源性为97.3%;与2009年流行于美国、加拿大、印度和俄罗斯的D4基因型麻疹野病毒的核苷酸和氨基酸同源性分别为98.0%～100%和97.3%～100%。结果表明,在本次输入性麻疹病例中分离到的麻疹病毒属于D4基因型,这对积累我国麻疹分子流行病学基线数据具有很重要的意义,同时也有助于监测和阐明麻疹病毒的传播途径。

关键词(KeyWords)： 麻疹病毒;病毒学监测;D4基因型;亲缘性分析

Abstract:

Keywords:

基金项目(Foundation): 重大传染病防治科技重大专项(2009ZX10004-202,2009ZX10004-201,2008ZX10004-014-5,2008ZX10004-008)

作者(Authors): 王慧玲;郑蕾;王骥涛;郜慧;张燕;孔晓慧;许文波;

DOI: 10.13242/j.cnki.bingduxuebao.002072

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