唐静;张曙霞;张靖;李希妍;刘佳;王大燕;

• 1:中国疾病预防控制中心病毒病预防控制所世界卫生组织流感参比和研究合作中心

摘要(Abstract)：

人感染高致病性H7N9禽流感病毒（Highly pathogenic avian influenza H7N9,HPAI H7N9）神经氨酸酶抑制剂（Neuraminidase inhibitors,NAIs）耐药株在哺乳动物细胞中适应性较好，是其导致病死率高的原因之一。本文研究HPAI H7N9耐药株对流感病毒敏感细胞MDCK细胞转录的影响。用0.1 MOI含NAIs耐药突变位点（NA R292K,E119V）的HPAI H7N9重组病毒及其敏感株感染MDCK细胞，于感染后0h、7h和24h取样，进行RNA测序并分析。结果三株病毒感染细胞0h和7h时，各组差异表达基因（Differentially expressed genes,DEGs）较少。24h时敏感株、R292K株和E119V株感染后的DEGs均显著增加，数量相似，分别为10 508、10 663和10 711，其中三者共同DEGs为83%～85%，每组特有DEGs为3%～8%。3株病毒感染细胞24 h时DEGs涉及的KEGG通路分类及各通路下基因个数及所占比例均较相似，且3者前20条KEGG通路中多数（11条）通路相同。结果说明，含R292K或E119V耐药突变位点的HPAI H7N9病毒与其敏感株相似地能在MDCK细胞中引起较强烈的转录改变并影响细胞代谢及免疫通路。

关键词(KeyWords)： 高致病性H7N9禽流感病毒;神经氨酸酶抑制剂耐药株;292K;119V;转录组学

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金青年基金（项目号：82102370），题目：人感染高致病性H7N9禽流感病毒神经氨酸酶抑制剂耐药机制及替代药物的研究~~

作者(Authors): 唐静;张曙霞;张靖;李希妍;刘佳;王大燕;

DOI: 10.13242/j.cnki.bingduxuebao.004145

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