元顺女;朴美花;金丹;

• 1:延边大学附属医院
• 2:延吉市医院

摘要(Abstract)：

乙肝病毒X蛋白（Hepatitis B virus X protein,HBx）高表达与乙肝相关肝癌的发病密切相关，但HBx发挥促癌作用的机制并不清楚。环加氧酶2(Cyclooxygenase,COX2)具有促进肝癌细胞增殖的功能，乙肝相关肝癌中COX2的表达增加且与HBx呈正相关，提示上调COX2可能是HBx促进肝癌细胞增殖的分子机制。为了阐明HBx是否通过上调COX2促进肝癌细胞增殖，本实验培养了肝癌HepG2细胞并分为对照组、转染pcDNA3.1-HBx质粒的HBx组、转染NC siRNA的si-NC组、转染NC siRNA及pcDNA3.1-HBx质粒的si-NC+HBx组、转染COX2 siRNA及pcDNA3.1-HBx质粒的si-COX2+HBx组。检测细胞增殖活力OD_(490nm)的水平，COX2、B淋巴细胞瘤2基因（BCL2）、生存素（Survivin）的表达量，前列腺素E2（英文名，PGE2）的含量。实验结果显示，HBx组的OD_(490nm)水平，细胞中COX2、BCL2、Survivin的表达量，培养基中PGE2的含量均高于对照组；si-COX2+HBx组的OD_(490nm)水平，细胞中COX2、BCL2、Survivin的表达量，培养基中PGE2的含量均低于si-NC+HBx组。以上结果表明，HBx促进肝癌细胞增殖的作用部分由上调COX2表达所介导。本实验阐明了上调COX2在HBx促进肝癌细胞增殖中的作用，这为今后研究HBx发挥促癌作用的分子机制以及乙肝相关肝癌的发病机制提供了新思路。

关键词(KeyWords)： 乙肝相关肝癌;乙肝病毒X蛋白(HBx);环氧合酶2(COX2);前列腺素E2(PGE2);细胞增殖

Abstract:

Keywords:

基金项目(Foundation):

作者(Authors): 元顺女;朴美花;金丹;

DOI: 10.13242/j.cnki.bingduxuebao.004042

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