戊型肝炎病毒基因1型和基因4型中和表位区域分子差异研究The Molecular Differences between Genotype 1 and Genotype 4 of HEV in Their Neutralization Region
郭清顺;葛胜祥;熊君辉;闫强;李少伟;顾颖;徐平东;史维国;张军;夏宁邵;
摘要(Abstract)：
戊型肝炎病毒(HEV)根据易感宿主的差别可以分为两大类:一类只分离自人的H(Human)类,包括HEV-1和HEV-2;一类为人畜共患的Z(Zoonosis)类,包括HEV-3和HEV-4。本研究通过比较这两类HEV的ORF2aa368606区段,发现存在4个类保守的差异位点,均位于HEV的主要中和表位区域aa459606区段,发现存在4个类保守的差异位点,均位于HEV的主要中和表位区域aa459606,分别是aa483、aa492、aa497和aa599;对这四个位点进行定点替换突变,以一组能够捕获HEV-1和/或HEV-4的单克隆抗体比较各种突变体的免疫反应性,结果表明仅aa497的差异造成了这两类HEV中和表位构象的部分差异,提示aa497及其相关的病毒表面结构差异在H类和Z类HEV宿主选择中可能扮演重要角色。
关键词(KeyWords)： 戊型肝炎病毒;宿主差异;中和表位
基金项目(Foundation): 863计划(2005DFA30820);; 国家自然科学基金(30500092);; 国家自然科学基金(30600106);; 福建省科技重大专项(2004YZ01-1)
作者(Authors): 郭清顺;葛胜祥;熊君辉;闫强;李少伟;顾颖;徐平东;史维国;张军;夏宁邵;
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