一株MLB1型星状病毒全基因组序列分析Genomic Characterization of A MLB1 Astrovirus Strain in China
王雅璐;秦萌;高升辉;李静欣;毛彤瑶;李丹地;李利利;赵璐;李金松;吴建军;段招军;
摘要(Abstract)：
了解我国MLB1型星状病毒基因组特征，为星状病毒腹泻的防控提供基础数据。使用星状病毒通用引物筛查北京市丰台区2017年72份儿童腹泻监测病例标本；扩增MLB星状病毒全基因组序列，采用在线比对、进化、重组等生物学信息学方法分析MLB1的全基因组序列。从72份标本中检出2份1型星状病毒（2.78%）和1份MLB1型星状病毒（MLB1-FT）(1.39%)。MLB1-FT与GenBank已报道的MLB1型星状病毒全基因组相似性为92.51%～98.28%;MLB1-FT的三个ORF区，仅5个位点氨基酸与其他株均不相同。依据其ORF2部分序列的进化分析，MLB1可分为A和B两个组，其中A组可分为a和b亚组，MLB1-FT为A组中的b亚组。未发现MLB1-FT具有重组现象。MLB1-FT具有星状病毒特征性的保守基序，部分保守基序与其他型别星状病毒不一样，为MLB1星状病毒特有。MLB1-FT具备新型星状病毒MLB的典型特征。我国新型星状病毒监测力度较弱，应加强监测。
关键词(KeyWords)： 新型星状病毒;MLB1;腹泻病毒
基金项目(Foundation): 国家科技重大专项（项目号：2018ZX10201002）,题目：基于海量多元大数据的突发急性传染病时空多尺度预测预警模型与应用示范的构建~~
作者(Authors): 王雅璐;秦萌;高升辉;李静欣;毛彤瑶;李丹地;李利利;赵璐;李金松;吴建军;段招军;
DOI: 10.13242/j.cnki.bingduxuebao.004166
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