李瑞芳,于志芬,黄俏,郭春阳,李雪

• 1:内蒙古师范大学物理与电子信息学院

摘要(Abstract)：

以A型流感病毒为研究样本,分析了同义密码子使用偏性对RNA二级结构的影响,为进一步研究同义密码子存在的意义及A型流感病毒RNA特征提供一些理论依据。收集整理了NCBI中收录的全部A型流感病毒的核酸序列信息,计算了每一条核酸序列的RNA二级结构,计算出RNA环结构含量和茎结构含量及折叠自由能。在此基础上,计算了RNA二级结构的柔性。同时,计算了每一条核酸序列的相对同义密码子使用值。由此,建立了A型流感病毒RNA二级结构数据库。分析每条核酸序列的相对同义密码子使用值与RNA的环结构、茎结构及柔性之间的关系。分析结果表明,50%的氨基酸的相对同义密码子使用值与RNA茎结构含量和环结构含量显著相关;60%的氨基酸的相对同义密码子使用值与单位平均折叠自由能显著相关;50%的氨基酸的相对同义密码子使用值与RNA柔性显著相关。进一步分析发现,与茎结构含量和环结构含量都显著相关的密码子,它们的相对同义密码子使用值与两种结构含量的相关性截然相反,而且发现,在所选的参量中,RNA柔性与相对同义密码子使用值显示出更好的相关性。结果证实,对于A型流感病毒,同义密码子的使用偏性对RNA二级结构存在很大的影响。

关键词(KeyWords)： A型流感病毒;同义密码子使用偏性;RNA二级结构;RNA柔性;折叠自由能

Abstract:

Keywords:

基金项目(Foundation): 内蒙古自治区自然科学基金项目(项目号:2016MS0362),题目:氨基酸特性及mRNA序列和结构对相应蛋白质折叠速率的影响

作者(Author): 李瑞芳,于志芬,黄俏,郭春阳,李雪

DOI: 10.13242/j.cnki.bingduxuebao.003076

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