南京地区诺如病毒GII.4 Sydney2012[P16]分子生物学特征研究Evolutionary Analysis of Norovirus GII.4 Sydney 2012[P16] in Outbreaks of Acute Gastroenteritis in Nanjing,China
王璇;雍玮;刘品;斯佳丽;龚红瑾;丁洁;
摘要(Abstract):
2016年1月-2021年12月,南京市急性胃肠炎暴发主要由GII.2[P16]引起,但2021年11月底,由GII.4Sydney2012[P16]引起的暴发显著上升。为了解GII.4 Sydney2012[P16]分子生物学特性,对监测期间204起急性胃肠炎暴发中收集到的3 129份样本进行荧光定量PCR检测,检出1 006份诺如病毒阳性样本,对部分阳性样进行一步法RT-PCR扩增、测序,共检出10种GII型诺如病毒,随机选取19株GII.4 Sydney2012[P16],结合7株GII.4Sydney2012[P31]、31株GII.2[P16]南京株及2015-2021年国内外参考株进行序列比对和进化分析。19株GII.4Sydney2012[P16]同源性达99.7%~100.0%,与参考株同源性达96.6%~99.3%。进化树分析显示,GII.P16虽与不同GII型诺如病毒组合,但进化距离上并未发生较大变化。与不同聚合酶区结合的GII.4 Sydney 2012在同一分支上,GII.4 Sydney 2012[P16]在进化距离上与GII.4 Sydney 2012[P4 NewOrleans2009]更为接近。P2区的A、B、D和NERK位点均发生改变,所有GII.4的373氨基酸残基处均检测到显著的阳性选择。GII.P16虽未发生较大改变,但通过与GII.4 Sydney2012重组,呈现加速在人群中流行的分子特征。而GII.4 Sydney2012通过改变抗原表位位点影响了抗原性和配体结合特性,显著促进了病毒的传播。因此GII.4 Sydney2012[P16]是否会取代GII.2[P16]成为南京地区流行株,抑或被其他GII.4所替代,今后需要对更为广泛的潜在感染人群进行监测,及早发现早期新型诺如病毒。
关键词(KeyWords): 诺如病毒;急性胃肠炎暴发;基因型别
基金项目(Foundation): 江苏省卫生健康委医学科研立项项目(项目号:H2019019),题目:诺如病毒深度测序与分子溯源技术研究及应用~~
作者(Authors): 王璇;雍玮;刘品;斯佳丽;龚红瑾;丁洁;
DOI: 10.13242/j.cnki.bingduxuebao.004221
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