基因Ⅲ型新城疫病毒感染鸡外周血单个核细胞后的靶细胞分析Target Cells Analysis of Genotype Ⅲ Newcastle Disease Virus in Chicken PBMC
孙一帆;陆小龙;王晓泉;胡顺林;刘秀梵;刘晓文;
摘要(Abstract)：
新城疫(ND)是鸡新城疫强毒株引起的一种鸡的烈性传染病,目前疫苗接种是防治该病的主要手段。临床上曾分离到与中等毒力疫苗株Mukteswar高度同源的强毒株JS/7/05/Ch,其通过静脉注射后毒力显著增强。为了探究JS/7/05/Ch经血液途径毒力增强的机制,本研究选用鸡外周血单个核细胞(PBMC)作为研究模型,分析基因Ⅲ型NDV在PBMC中的靶细胞。细胞分选结果表明病毒感染可诱导PBMC中单核细胞的增殖。实验组病毒感染后单核细胞相对占比上升,感染后1d Mukteswar组(Muk组)单核细胞占比16.3%,JS/7/05/Ch组(JS组)为13.21%,而对照组单核细胞仅占比3.18%。荧光定量PCR测定分选后各细胞中的病毒载量,感染后3d JS组单核细胞中的病毒含量与感染后1d相比极显著性增加(P<0.01)。感染后1d病毒以感染淋巴细胞为主,而在感染后3d单核细胞的相对病毒含量均超过淋巴细胞占据主导地位,Muk组和JS组分别为54.2%和60.2%。综上所述,基因Ⅲ型NDV在PBMC中的靶细胞是单核巨噬细胞。这为进一步研究这对基因Ⅲ型模式病毒毒力差异的机制奠定了一定基础。
关键词(KeyWords)： 新城疫病毒(NDV);外周血单个核细胞;单核细胞;流式细胞分析;荧光定量PCR
基金项目(Foundation): 国家自然科学基金面上项目（项目号：31873021），题目：新城疫病毒Ⅰ系苗（Mukteswar）的进化及其毒力增强的分子机制~~
作者(Authors): 孙一帆;陆小龙;王晓泉;胡顺林;刘秀梵;刘晓文;
DOI: 10.13242/j.cnki.bingduxuebao.004075
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