陈韵颖,吴英,汪铭书,程安春

• 1:四川农业大学动物医学院
• 2:四川农业大学动物医学院禽病防治研究中心
• 3:四川农业大学预防兽医研究所
• 4:四川农业大学动物疫病与人类健康四川省重点实验室

摘要(Abstract)：

单纯疱疹病毒1型(Herpes simplex virus 1,HSV-1)感染细胞蛋白22(Infected Cell Protein 22,ICP22)是Us1基因编码的一种翻译后修饰多功能蛋白,为HSV-1的五种立即早期蛋白之一。HSV-1ICP22能与不同的细胞和病毒成分相互作用来执行不同的功能,包括改变RNA聚合酶Ⅱ(RNA polymerase II,RNAPⅡ/PolⅡ)的磷酸化状态、参与抵抗细胞对病毒复制的消极作用、引起细胞周期蛋白A和B水平降低、介导修饰拓扑异构酶Ⅱα、参与胞核内病毒诱导的分子伴侣富集(Virus-induced chaperone-enriched,VICE)区域的形成及促进病毒新生核衣壳的初次包装。这些作用大多与调节病毒在胞核中有效复制相关。此外,HSV-1ICP22还在限制细胞中的病毒复制、病毒致病力和潜伏感染建立过程中发挥重要作用,但ICP22发挥这些作用的机制尚未知。本文就上述目前国内外对HSV ICP22的研究进展作一综述,以期为后续研究提供参考。

关键词(KeyWords)： 单纯疱疹病毒1型(HSV-1);感染细胞蛋白22(ICP22);RNA聚合酶Ⅱ(PolⅡ);p53;细胞周期蛋白依赖性激酶1(cdk1);病毒诱导的伴侣富集;核出芽

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金(项目号:31602079);题目:双拷贝ICP22对鸭瘟病毒转录调控的影响及功能位点解析;; 四川省教育厅重点项目(项目号:17ZA0309);题目:鸭瘟病毒单、双拷贝ICP22基因在病毒基因转录调控中的功能初探~~

作者(Author): 陈韵颖,吴英,汪铭书,程安春

DOI: 10.13242/j.cnki.bingduxuebao.003296

参考文献(References)：

• [1]Kolb A W,Schmidt T R,Dyer D W,Brandt C R.Sequence variation in the herpes simplex virus Us1ocular virulence determinant[J].Invest Ophthalmol Vis Sci,2011,52(7):4630-4638.
• [2]Mostafa H H,Davido D J.Herpes simplex virus 1ICP22but not Us 1.5is required for efficient acute replication in mice and VICE domain formation[J].J Virol,2013,87(24):13510-13519.
• [3]Smithdonald B A,Roizman B.The interaction of herpes simplex virus 1 regulatory protein ICP22 with the cdc25Cphosphatase is enabled in vitro by viral protein kinases Us3and UL13[J].J Virol,2008,82(9):4533.
• [4]Durand L O,Advani S J,Poon A P W,Roizman B.The carboxyl-terminal domain of RNA polymerase II is phosphorylated by a complex containing cdk9and infected-cell protein 22of herpes simplex virus 1[J].J Virol,2005,79(11):6757-6762.
• [5]Lin F S,Ding Q,Guo H,Zheng A C.The herpes simplex virus type 1infected cell protein 22[J].Virol Sin,2010,25(1):1-7.
• [6]Maruzuru Y,Fujii H,Oyama M,Kozuka-Hata H,Kato A,Kawaguchi Y.Roles of p53in herpes simplex virus 1replication[J].J Virol,2013,87(16):9323.
• [7]Rice S A,Davido D J.HSV-1ICP22:hijacking host nuclear functions to enhance viral infection[J].Future Microbiol,2013,8(3):311-321.
• [8]Fraser K A,Rice S A.Herpes simplex virus type 1infection leads to loss of serine-2phosphorylation on the carboxyl-terminal domain of RNA polymerase II[J].J Virol,2005,79(17):11323-11334.
• [9]Fraser K A,Rice S A.Herpes simplex virus immediateearly protein ICP22triggers loss of serine 2-phosphorylated RNA polymerase II[J].J Virol,2007,81(10):5091-5101.
• [10]Zaborowska J,Isa N F,Murphy S.P-TEFb goes viral[J].Inside the Cell,2016,1(2):106.
• [11]Durand L O,Roizman B.Role of cdk9in the optimization of expression of the genes regulated by ICP22of herpes simplex virus 1[J].J Virol,2008,82(21):10591.
• [12]Guo L,Wu W J,Liu L D,Wang L C,Zhang Y,Wu L Q,Guan Y,Li Q H.Herpes simplex virus 1ICP22Inhibits the transcription of viral gene promoters by binding to and blocking the recruitment of P-TEFb[J/OL].PLoS One,2012,7(9):e45749.
• [13]Bastian T W,Rice S A.Identification of sequences in herpes simplex virus type 1ICP22that influence RNA polymerase II modification and viral late gene expression[J].J Virol,2009,83(1):128.
• [14]Zaborowska J,Baumli S,Laitem C,O’Reilly D,Thomas P H,O’Hare P,Murphy S.Herpes simplex virus 1(HSV-1)ICP22protein directly interacts with cyclin-dependent kinase(CDK)9to inhibit RNA polymerase II transcription elongation[J/OL].PLoS One,2014,9(9):e107654.
• [15]Fox H L,Dembowski J A,Deluca N A.A herpesviral immediate early protein promotes transcription elongation of viral transcripts[J/OL].MBio,2017,8(3):e00745-17.
• [16]Kalamvoki M,Roizman B.Circadian CLOCK histone acetyl transferase localizes at ND10nuclear bodies and enables herpes simplex virus gene expression[J].Proc Natl Acad Sci USA,2010,107(41):17721-17726.
• [17]Kalamvoki M,Roizman B.The histone acetyltransferase CLOCK is an essential component of the herpes simplex virus 1 transcriptome that includes TFIID,ICP4,ICP27and ICP22[J].J Virol,2011,85(18):9472-9477.
• [18]游韶平.单纯疱疹病毒Ⅱ型感染细胞多肽ICP22对Vero细胞周期作用的研究[D].华南理工大学,2014.
• [19]Advani S J,Weichselbaum R R,Roizman B.Herpes simplex virus 1activates cdc2to recruit topoisomerase II alpha for post-DNA synthesis expression of late genes[J].Proc Natl Acad Sci USA,2003,100(8):4825-4830.
• [20]Bastian T W,Livingston C M,Weller S K,Rice S A.Herpes simplex virus type 1immediate-early protein ICP22is required for VICE domain formation during productive viral infection[J].J Virol,2010,84(5):2384-2394.
• [21]Livingston C M,Ifrim M F,Cowan A E,Weller S K.Virus-Induced Chaperone-Enriched(VICE)domains function as nuclear protein quality control centers during HSV-1infection[J/OL].PLoS Pathog,2009,5(10):e1000619.
• [22]Li L,Johnson L A,Daiju J Q,Sandrigoldin R M.Hsc70focus formation at the periphery of HSV-1transcription sites requires ICP27[J/OL].PLoS One,2008,3(1):e1491.
• [23]Liu Z,Kato A,Shindo K,Noda T,Sagara H,Kawaoka Y,Arii J,Kawaguchi Y.Herpes simplex virus 1 UL47interacts with viral nuclear egress factors UL31,UL34,and Us3and regulates viral nuclear egress[J].J Virol,2014,88(9):4657.
• [24]Maruzuru Y,Shindo K,Liu Z,Oyama M,KozukaHata H,Arii J,Kato A,Kawaquchi Y.Role of herpes simplex virus 1immediate early protein ICP22in viral nuclear egress[J].J Virol,2014,88(13):7445-7454.