兔出血症病毒复制子的构建及其在RK-13细胞中的复制Construction of Rabbit Hemorrhagic Disease Virus Replicons and Its Replication in RK-13 Cells
刘光清;倪征;云涛;余斌;朱金梅;华炯钢;陈剑平;
摘要(Abstract)：
为研究兔出血症病毒(RHDV)的复制机制、病毒与宿主之间的相互作用以及致病机制等,创建一个安全、有效的技术平台,在前期构建的RHDV侵染性克隆基础上,将病毒的衣壳蛋白编码区删除,保留了RHDV复制必需的所有蛋白酶基因和两端的非编码区,构建了RHDV复制子。试验结果证明,将该复制子RNA导入RK13细胞中后,能够进行高水平的复制和表达。
关键词(KeyWords)： 兔出血症病毒;复制子;衣壳蛋白;RK-13细胞
基金项目(Foundation): 国家自然科学基金项目(30670074);; 浙江省自然科学基金(Y305074)
作者(Authors): 刘光清;倪征;云涛;余斌;朱金梅;华炯钢;陈剑平;
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