袁芳,陈慧,马江涛,马学旻,詹军

• 1:宁夏疾病预防控制中心

摘要(Abstract)：

研究2013~2015年宁夏地区普通手足口病(hand,foot and mouth disease,HFMD)病例中柯萨奇病毒A组6型(Coxsackievirus A6,CV-A6)分离株基因特征。将2013~2015年收集的普通HFMD病例标本中所有非肠道病毒A71型(enterovirus,71)和非柯萨奇病毒16型(Coxsackievirus A16,CV-A16)的其它肠道病毒(Enterovirus,EV)核酸阳性标本,使用RD细胞进行病毒分离,获得的毒株使用兼并引物进行VP1区基因扩增和测序,测序结果编辑后通过BLAST比对进行型别鉴定,选择CV-A6毒株进行基因进化分析。2013~2015年共收集非EV71和非CV-A16的EV阳性标本998份,共分离到227株毒株,其中61株鉴定为CV-A6,同源性分析表明宁夏61株CVA6间核苷酸和氨基酸序列同源性分别是96.1%~99.8%和98%~100%,与Gudla原型株同源性为82%~83.5%和93.8%~95.7%,遗传距离较远。CV-A6成为宁夏地区2013年和2015年其它肠道病毒中导致HFMD的主要病原体与国内流行的CV-A6具有较高的同源性,流行趋势一致,并且在宁夏地区存在多个传播链。

关键词(KeyWords)： 手足口病(HFMD);病原;柯萨奇病毒A组6型(CV-A6);进化分析

Abstract:

Keywords:

基金项目(Foundation): 宁夏回族自治区科研支撑项目(项目号:2013ZYS119),题目:新发传染病预警网络与未知病毒病原学检测平台的建立

作者(Author): 袁芳,陈慧,马江涛,马学旻,詹军

DOI: 10.13242/j.cnki.bingduxuebao.003065

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