昆明市某医院2013~2014年住院婴幼儿感染星状病毒的分子流行病学研究Molecular Epidemiology of Astrovirus Infection Among Hospitalized Infants in Kunming City,China,2013~2014
杨思达,任禹衡,邓丽丽,杨景晖,熊永英,井申荣,李利
摘要(Abstract):
探讨昆明市某医院2013~2014年住院婴幼儿星状病毒(human astrovirus,HAstV)腹泻的感染情况,分析其分子流行病学特征。通过逆转录RT-PCR法,对63份婴幼儿腹泻样品和42份非腹泻样品进行星状病毒分型鉴定;并扩增1株HAstV全基因组序列。结果在63份样品中,HAstV检出率为41.27%(26/63),以HAstV1型为主要流行株;42份对照组中,HAstV感染率为2.38%(1/42);扩增获得1株HAstV全基因组序列,进化树分析为HAstV1型。本研究获得了昆明地区婴幼儿星状病毒腹泻流行病学数据,为昆明地区星状病毒腹泻的感染、控制提供了重要的理论依据。
关键词(KeyWords): 婴幼儿腹泻;星状病毒;分子流行病学
基金项目(Foundation): 云南省应用基础科技计划项目(2010ZC213);; 大学生创新训练项目(201510674085)
作者(Author): 杨思达,任禹衡,邓丽丽,杨景晖,熊永英,井申荣,李利
DOI: 10.13242/j.cnki.bingduxuebao.003073
参考文献(References):
- [1]Enserink R,Van d W C,Bruijningverhagen P,Van A L,Mughinigras L,Duizer E,Kortbeek T,Scholts R,Nagelkerke N,Henriette A S.Gastroenteritis attributable to 16enteropathogens in children attending day care:significant effects of rotavirus,norovirus,astrovirus,cryptosporidium and giardia[J].Pediatr Infect Dis J,2015,34(1):5-10.
- [2]Yu J,Xiang H.Molecular epidemiology of virus diarrhea among infants and young children in Lanzhou[J].Chin J Pract Pediat,2006,21(1):15-18.
- [3]Amaral M S,Estevam G K,Penatti M,Lafontaine R,Lima I C,Spada P K,Gabbay Y B,Matos N B.The prevalence of norovirus,astrovirus and adenovirus infections among hospitalised children with acute gastroenteritis in Porto Velho,state of Rondonia,western Brazilian Amazon[J].Mem Inst Oswal Cruz,2015,110(2):215-221.
- [4]Brown J R,Morfopoulou S,Hubb J,Emmett W A,Winnie I,Shah D,Brooks T,Paine S,Anderson G,Virasami A.Astrovirus VA1/HMO-C:an increasingly recognized neurotropic pathogen in immunocompromised patients[J].Clin Infect Dis,2015,60(6):881-888.
- [5]Naccache S N,Peggs K S,Mattes F M,Phadke R,Garson P,Samayoa E,Federman S,Miller S,Lunn M P.Diagnosis of neuroinvasive astrovirus infection in an immunocompromised adult with encephalitis by unbiased next-generation sequencing[J].Clin Infect Dis,2015,60(6):919-923.
- [6]张倩,黎曼侬,王喆.昆明市腹泻婴幼儿星状病毒感染检测分析[J].中国社区医师医学专业,2011,13(26):40-41.
- [7]朱攀.昆明市小儿腹泻相关病毒的分子流行病学研究及病毒基因组克隆[J].昆明:昆明理工大学,2013.
- [8]Galdiero E,Marinelli A,Pisciotta M G,Pagliara I,Monteforte E S D,Liguori G.Reverse transcriptasePCR for the detection of Astrovirus in children with nosocomial acute diarrhoea in Naples,Italy[J].Med Mal Infect,2005,35(4):213-217.
- [9]Jeong A Y,Jeong H S,Jo M Y,Jung S Y,Lee M S,Lee J S,Jee Y M,Kim J H,Cheon D S..Molecular epidemiology and genetic diversity of human astrovirus in South Korea from 2002to 2007[J].Clin Microbiol Infect,2011,17(3):404-408.
- [10]Zhang X E,Li D D,Li X,Yang X D,Cai K,Wang Y X,Yang L B,Duan Z.Etiological and epidemiological study on viral diarrhea among children in Changchun[J].Chin J Clin Med Virol,2012,26(1):5-7.
- [11]Hye Sook J,Ahyong J,Doo-Sung C.Epidemiology of astrovirus infection in children[J].Korean J Pediatr,2012,55(3):77-82.
- [12]Benedictis P D,Schultz-Cherry S,Burnham A,Cattoli G.Astrovirus infections in humans and animals-molecular biology,genetic diversity,and interspecies transmissions[J].Infect Genet Evol,2013,11(7):1529-1544.
- [13]Zhang Y H,Luo X F,Wang Q.Analysis of astrovirus infection in 413infants with acute diarrhea in Shenzhen City[J].Chin Trop Med,2011,29.
- [14]姜盛,李建名,张星,吴炜,杨雪萍.吉林、兰州地区婴幼儿人星状病毒腹泻的分子流行病学研究[J].蚌埠医学院学报,2013,38(5):607-609.
- [15]Li C Y,Liu N,Guo W D,Yu Q,Wang W R,Song Z Z,Yan H,Luo Y,Lu A T,Li H Y.Outbreak of neonatal gastroenteritis associated with astrovirus serotype 1at a hospital in Inner Mongolia,China[J].J Clin Microbiol,2010,48(11):4306-4309.
- [16]王承训,黎伟明,孙利伟,杨显达.长春市2013年小儿病毒性腹泻病原与临床特征研究[J].吉林医学,2014,(22):3301-3303.
- [17]赵雪琴,寿钧,张睿.2012年-2013年杭州地区婴幼儿病毒性腹泻监测结果分析[J].中国卫生检验杂志,2014,(32):7145-7146.
- [18]Pankovics P,Boros,Rovács M,Nagy E,Krisztián E,Vollain M,Reuter G..First detection of human astrovirus in gastroenteritis outbreak in Hungary[J].Orv Hetil,2011,152(2):45-50.
- [19]Jakab F,Meleg E,Bányai K,Melegh B,Tímár L,Péterfai J,Szücs G..One-year survey of astrovirus infection in children with gastroenteritis in a large hospital in Hungary:occurrence and genetic analysis of astroviruses[J].J Med Virol,2004,74(1):71-77.