吴仕伟,付小哲,林强,刘礼辉,梁红茹,黄志斌,李宁求

• 1:中国水产科学研究院珠江水产研究所农业部渔用药物创制重点实验室广东省水产动物免疫技术重点实验室
• 2:上海海洋大学水产与生命学院
• 3:淡水水产健康养殖湖北省协同创新中心

摘要(Abstract)：

病毒挟持宿主细胞代谢以实现自身的复制和增殖,糖类作为宿主细胞最主要能量来源及大分子物质合成重要碳源,在病毒增殖过程中其代谢受到严密调控。本文从病毒感染影响葡萄糖转运、糖代谢(糖酵解、磷酸戊糖途径、糖异生)以及胰岛素信号通路等3个方面概述病毒感染影响宿主细胞葡萄糖代谢研究进展,以期为病毒影响细胞葡萄糖代谢研究提供参考。

关键词(KeyWords)： 病毒感染;葡萄糖转运;糖代谢;机制

Abstract:

Keywords:

基金项目(Foundation): 中国水产科学研究院基本科研业务费专项(2015B05XK03),题目:细胞自噬在ISKNV和SCRV复制感染中作用研究;; 国家自然科学基金(31502201),题目:P53介导的鳜传染性脾肾坏死病毒免疫逃逸分子机制研究;; 广东省海洋渔业科技推广专项(A201501B12),题目:鳜虹彩病毒病高毒价细胞灭活疫苗快速制备关键技术研究

作者(Author): 吴仕伟,付小哲,林强,刘礼辉,梁红茹,黄志斌,李宁求

DOI: 10.13242/j.cnki.bingduxuebao.003080

参考文献(References)：

• [1]Warburg O.On the origin of cancer cells[J].Science,1956,123(3191):309-314.
• [2]Eagle H,Habel K.The nutritional requirements for the propagation of poliomyelitis virus by the HeLa cell[J].The Journal of experimental medicine,1956,104(2):271-287.
• [3]Klemperer H.Glucose breakdown in chick embryo cells infected with influenza virus[J].Virology,1961,13(1):68-77.
• [4]Manel N,Kim F J,Kinet S,Taylor N,Sitbon M,Battini J L.The ubiquitous glucose transporter GLUT-1is a receptor for HTLV[J].Cell,2003,115(4):449-459.
• [5]Lewis V J,Scott L V.NUTRITIONAL REQUIREMENTS FOR THE PRODUCTION OF HERPES SIMPLEX VIRUS I.Influence of Glucose and Glutamine on Herpes Simplex Virus Production by HeLa Cells[J].Journal of Bacteriology,1962,83(3):475-482.
• [6]Courtney R J,Steiner S M,Benyesh-Melnick M.Effects of 2-deoxy-D-glucose on herpes simplex virus replication[J].Virology,1973,52(2):447-455.
• [7]Tritel M,Resh M D.The late stage of human immunodeficiency virus type 1assembly is an energy-dependent process[J].Journal of Virology,2001,75(12):5473-5481.
• [8]El-Bacha T,Menezes M M,e Silva M C A.,Sola-Penna M,Da Poian A T.Mayaro virus infection alters glucose metabolism in cultured cells through activation of the enzyme 6-phosphofructo 1-kinase[J].Molecular and cellular biochemistry,2004,266(1-2):191-198.
• [9]Landini M P.Early enhanced glucose uptake in human cytomegalovirus-infected cells[J].J Gen Virol,1984,65:1229-1232.
• [10]Johnson R A,Wang X,Ma X L,Huong S M,Huang E S.Human cytomegalovirus up-regulates the phosphatidylinositol 3-kinase(PI3-K)pathway:inhibition of PI3-K activity inhibits viral replication and virus-induced signaling[J].J Virol,2001,75:6022-6032.
• [11]Munger J,Bajad S U,Coller H A,Shenk T,Rabinowitz J D.Dynamics of the cellular metabolome during human cytomegalovirus infection[J/OL].PLoS Pathogens.2006,2:1165-1175.
• [12]Pan Y R,Vatsyayan J,Chang Y S,Chang H Y.Epstein-Barr virus latent membrane protein 2A upregulates UDP‐glucose dehydrogenase gene expression via ERK and PI3K/Akt pathway[J].Cellular Microbiology,2008,10(12):2447-2460.
• [13]Delgado T,CarrollP A,Punjabi A S,Margineantu D,Hockenbery D M,Lagunoff M.Induction of the Warburg effect by Kaposi′s sarcoma herpesvirus is required for the maintenance of latently infected endothelial cells[J].Proceedings of the National Academy of Sciences,2010,107(23):10696-10701.
• [14]Delgado T,Sanchez E L,Camarda R,Lagunoff M.Global metabolic profiling of infection by an oncogenic virus:KSHV induces and requires lipogenesis for survival of latent infection[J/OL].PLoS pathogens,2012,8(8):e1002866.
• [15]Yogev O,Lagos D,Enver T,Boshoff C.Kaposi′s sarcoma herpesvirus microRNAs induce metabolic transformation of infected cells[J/OL].PLoS Pathog,2014,10(9):e1004400.
• [16]Chambers J W,Maguire T G,Alwine J C.Glutamine metabolism is essential for human cytomegalovirus infection[J].J Virol,2010,84(4):1867-1873.
• [17]Yu Y,Clippinger A J,Alwine J C.Viral effects on metabolism:changes in glucose and glutamine utilization during human cytomegalovirus infection[J].Trends in Microbiology,2011,19(7):360-367.
• [18]Vastag L,Koyuncu E,Grady S L.,Shenk T E,Rabinowitz J D.Divergent effects of human cytomegalovirus and herpes simplex virus-1on cellular metabolism[J/OL].PLoS Pathog,2011,7(7):e1002124.
• [19]Abrantes J L,Alves C M,Costa J,Almeida F C,SolaPenna M,Fontes C F L,Souza T M L.Herpes simplex type 1activates glycolysis through engagement of the enzyme 6-phosphofructo-1-kinase(PFK-1)[J].Biochimica et Biophysica Acta(BBA)-Molecular Basis of Disease,2012,1822(8):1198-1206.
• [20]孙丽杰,赵勇华,李树臣,于建武,康鹏,刘伟.丙型肝炎病毒核心蛋白下调AMP激活蛋白激酶导致肝细胞糖代谢紊乱[J].肝脏,2013,18(3):150-154.
• [21]Yu J W,Sun L J,Zhao Y H,Kang P,Yan B Z.Inhibition of silent information regulator 1induces glucose metabolism disorders of hepatocytes and enhances hepatitis C virus replication[J].Hepatology International,2013,7(2):524-532.
• [22]Sun L J,Yu J W,Wan L,Zhang X Y,Shi Y G,Chen M Y.Endocannabinoid system activation contributes to glucose metabolism disorders of hepatocytes and promotes hepatitis C virus replication[J].International Journal of Infectious Diseases,2014,23:75-81.
• [23]张汾燕,冯岩梅,朱跃科,张立洁,于红卫,李娟,付万发,孟庆华.葡萄糖激酶mRNA、葡萄糖转运蛋白2在慢性乙型重型肝炎患者糖代谢异常中的作用[J].临床肝胆病杂志,2013,08:607-610.
• [24]Barrero C A,Datta P K,Sen S,Deshmane S,Amini S,Khalili K,Merali S.HIV-1Vpr modulates macrophage metabolic pathways:a SILAC-based quantitative analysis[J].PLoS One,2013,8(7):e68376.
• [25]Thai M,Graham N A,Braas D,Nehil M,Komisopoulou E,Kurdistani S K,Christofk H R.Adenovirus E4ORF1-induced MYC activation promotes host cell anabolic glucose metabolism and virus replication[J].Cell Metabolism,2014,19(4):694-701.
• [26]Su M A,Huang Y T,Chen I T,Lee D Y,Hsieh Y C,Li C Y,Chiang Y A.An invertebrate warburg effect:a shrimp virus achieves successful replication by altering the host metabolome via the PI3K-Akt-mTOR pathway[J/OL].PLoS Pathog,2014,10(6):e1004196.
• [27]Chen I T,Aoki T,Huang Y T,Hirono I,Chen T C,Huang J Y,Wang H C.White spot syndrome virus induces metabolic changes resembling the warburg effect in shrimp hemocytes in the early stage of infection[J].J Virol,2011,85(24):12919-12928.
• [28]Allonso D,Andrade I S,Conde J N,Coelho D R,Rocha D C,da Silva M L,Mohana-Borges,R.Dengue virus NS1protein modulates cellular energy metabolism by increasing glyceraldehyde-3-phosphate dehydrogenase activity[J].J Virol,2015,89(23):11871-11883.
• [29]Perera R,Riley C,Isaac G,Hopf-Jannasch A S,Moore R J,Weitz K W,Kuhn R J.Dengue virus infection perturbs lipid homeostasis in infected mosquito cells[J].PLoS Pathog,2012,8(3):e1002584.
• [30]Noch E,Khalili K.Oncogenic viruses and tumor glucose metabolism:like kids in a candy store[J].Molecular Cancer Therapeutics,2012,11(1):14-23.
• [31]Munger J,Bennett B D,Parikh A,Feng X J,McArdle J,Rabitz H A,Rabinowitz,J D.Systems-level metabolic flux profiling identifies fatty acid synthesis as a target for antiviral therapy[J].Nature biotechnology,2008,26(10):1179-1186.
• [32]Ito T,Noguchi Y,Satoh S,Hayashi H,Inayama Y,Kitamura H.Expression of facilitative glucose transporter isoforms in lung carcinomas:its relation to histologic type,differentiation grade,and tumor stage[J].Modern pathology:an official journal of the United States and Canadian Academy of Pathology,Inc,1998,11(5):437-443.
• [33]江诚,谢俊,陈海峰.葡萄糖转运蛋白的转运机制研究[J].基因组学与应用生物学,2015,07:1372-1377.
• [34]滕继林,肖军.葡萄糖转运蛋白1的研究进展[J].生物学教学,2015,06:2-3.
• [35]Jin Q,Alkhatib B,Cornetta K,Alkhatib G.Alternate receptor usage of neuropilin-1and glucose transporter protein 1by the human T cell leukemia virus type 1[J].Virology,2010,396(2):203-212.
• [36]Huang H T,Chan H L,Shih T Y,Chen L L.A study of the role of glucose transporter 1(Glut1)in white spot syndrome virus(WSSV)infection[J].Fish&Shellfish Immunology,2015,46(2):305-314.
• [37]Gonnella R,Santarelli R,Farina A,Granato M,D’Orazi G,Faggioni A,Cirone,M.Kaposi sarcoma associated herpesvirus(KSHV)induces AKT hyperphosphorylation,bortezomib-resistance and GLUT-1plasma membrane exposure in THP-1 monocytic cell line[J].Journal of Experimental&Clinical Cancer Research,2013,32(1):1.
• [38]Becker Y,Tavor E,Asher Y,Berkowitz C,Moyal M.Effect of herpes simplex virus type-1UL41gene on the stability of mRNA from the cellular genes:β-actin,fibronectin,glucose transporter-1,and docking protein,and on virus intraperitoneal pathogenicity to newborn mice[J].Virus Genes,1993,7(2):133-143.
• [39]Carvalho E,Schellhorn S E,Zabolotny J M,Martin S,Tozzo E,Peroni O D,Kahn B B.GLUT4over expression or deficiency in adipocytes of transgenic mice alters the composition of GLUT4vesicles and the subcellular localization of GLUT4 and insulin-responsive aminopeptidase[J].Journal of Biological Chemistry,2004,279:21598-21605.
• [40]Seo J Y,Cresswell P.Viperin regulates cellular lipid metabolism during human cytomegalovirus infection[J/OL].PLoS Pathog,2013,9(8):e1003497.
• [41]Yu Y,Maguire T G,Alwine J C.Human cytomegalovirus activates glucose transporter 4expression to increase glucose uptake during infection[J].J Virol,2011,85(4):1573-1580.
• [42]Jin Q,Agrawal L,VanHorn-Ali Z,Alkhatib G.Infection of CD4+T lymphocytes by the human T cell leukemia virus type 1is mediated by the glucose transporter GLUT-1:evidence using antibodies specific to the receptor′s large extracellular domain[J].Virology,2006,349(1):184-196.
• [43]Jin Q,Agrawal L,VanHorn-Ali Z,Alkhatib G.GLUT-1-independent infection of the glioblastoma/astroglioma U87cells by the human T cell leukemia virus type 1[J].Virology,2006,353(1):99-110.
• [44]马丽华.1HNMR对单纯疱疹病毒1型感染神经胶质细胞的代谢组学研究[D].重庆医科大学,2012.
• [45]Nicholson J K,Lindon J C,Holmes E.′Metabonomics′:understanding the metabolic responses of living systems to pathophysiological stimuli via multivariate statistical analysis of biological NMR spectroscopic data[J].Xenobiotica,1999,29(11):1181-1189.
• [46]肖兰博.EB病毒介导的肿瘤细胞代谢改变及其重编程的分子机制研究[D].中南大学,2013.
• [47]董欣.鼻咽癌中糖酵解与脂肪酸β氧化异常活化的机制研究[D].中南大学,2014.
• [48]Xiao L,Hu Z Y,Dong X,Tan Z,Li W,Tang M Li,J.Targeting Epstein-Barr virus oncoprotein LMP1-mediated glycolysis sensitizes nasopharyngeal carcinoma to radiation therapy[J].Oncogene,2014,33(37):4568-4578.
• [49]Hollenbaugh J A,Munger J,Kim B.Metabolite profiles of human immunodeficiency virus infected CD4+T cells and macrophages using LC-MS/MS analysis[J].Virology,2011,415(2):153-159.
• [50]Ramière C,Rodriguez J,Enache L S,Lotteau V,AndréP,Diaz O.Activity of hexokinase is increased by its interaction with hepatitis C virus protein NS5A[J].J Virol,2014,88(6):3246-3254.
• [51]李玲,徐小洁,叶棋浓.蛋白质修饰与肿瘤糖代谢[J].中国科学:生命科学,2015,11:1101-1109.
• [52]Mazzon M,Peters N E,Loenarz C,Krysztofinska E M,Ember S W,Ferguson B J,Smith G L.A mechanism for induction of a hypoxic response by vaccinia virus[J].PNAS,2013,110(30):12444-12449.
• [53]Plas D,Talapatra S,Edinger A L,Rathmell J C,Thompson C B.Akt and Bcl-xL promote growth factor-independent survival through distinct effects on mitochondrial physiology[J].Journal of Biological Chemistry,2001,276(15):12041-12048.
• [54]Elstrom R L,Bauer D E,Buzzai M,Karnauskas R,Harris M H,Plas D R,Thompson C B.Akt stimulates aerobic glycolysis in cancer cells[J].Cancer Research,2004,64:3892-3899.
• [55]Gottlob K,Majewski N,Kennedy S,Kandel E,Robey R B,Hay N.Inhibition of early apoptotic events by Akt/PKB is dependent on the first committed step of glycolysis and mitochondrial hexokinase[J].Genes&Development,2001,15:1406-1418.
• [56]Miyamoto S,Murphy A N,Brown J H.Akt mediates mitochondrial protection in cardiomyocytes through phosphorylation of mitochondrial hexokinase-II[J].Cell Death Diff,2008,15:521-529.
• [57]Rathmell J C,Fox C J,Plas D R,Hammerman P S,Cinalli R M,Thompson C B.Akt directed glucose metabolism can prevent Bax conformation change and promote growth factor-independent survival[J].Mol Cell Biol,2003,23:7315-7328.
• [58]Baharuddin B,Zekry A,Simar D,Palmer C,Lloyd A.769Impaired t-cell glucose metabolism during chronic hepatitis c virus infection[J].Journal of Hepatology,2012,56:S302.
• [59]Diamond D L,Syder A J,Jacobs J M,Sorensen C M,Walters K A,Proll S C,Metz T O.Temporal proteome and lipidome profiles reveal hepatitis C virus-associated reprogramming of hepatocellular metabolism and bioenergetics[J/OL].PLoS Pathog,2010,6(1):e1000719.
• [60]孙英梅,许国蓉,陶剑平.丙型肝炎病毒核心蛋白引发肝脂肪变性机制的研究进展[J].国际病毒学杂志,2009,16(6):167-170.
• [61]刘问,钱冬,潘清清,严小军.WSSV对锯缘青蟹的致病性及血清酶指标影响[J].水生生物学报,2009,33(6):1112-1117.
• [62]Bechmann L P,Gastaldelli A,Vetter D,Patman G L,Pascoe L,Hannivoort R A,Lee Y M.Glucokinase links Krüppel-like factor 6to the regulation of hepatic insulinsensitivity in nonalcoholic fatty liver disease[J].Hepatology,2012,55(4):1083-1093.
• [63]Yu Y,Maguire T G,Alwine J C.ChREBP,aglucoseresponsive transcriptional factor,enhances glucose metabolism to support biosynthesis in human cytomegalovirus-infected cells[J].PNAS,2014,111(5):1951-1956.
• [64]Birungi G,Chen S M,Loy B P,Ng M L,Li S F Y.Metabolomics approach for investigation of effects of dengue virus infection using the EA.hy926cell line[J].Journal of Proteome Research,2010,9(12):6523-6534.
• [65]Radsak K D,Weder D.Effect of 2-deoxy-D-glucose on cytomegalovirus-induced DNA synthesis in human fibroblasts[J].J Gen Virol,1981,57(1):33-42.
• [66]McArdle J,Schafer X L,Munger J.Inhibition of calmodulin-dependent kinase kinase blocks human cytomegalovirus-induced glycolytic activation and severely attenuates production of viral progeny[J].J Virol,2011,85(2):705-714.
• [67]Fontaine K A,Camarda R,Lagunoff M.Vaccinia virus requires glutamine but not glucose for efficient replication[J].Journal of Virology,2014,88:4366-4374.
• [68]Fontaine K A,Sanchez E L,Camarda R,Lagunoff M.Dengue virus induces and requires glycolysis for optimal replication[J].Journal of Virology,2015,89(4):2358-2366.
• [69]刘文祥,汪涛,侯鹏.应用表达谱芯片筛选HCV NS5B基因转染Hun7.5细胞所致表达差异的糖、脂类代谢基因[J].国际病毒学杂志,2013,20(5):215-219.
• [70]Ralser M,Wamelink M M,Kowald A,Gerisch B,Heeren G,Struys E A,Krobitsch S.Dynamic rerouting of the carbohydrate flux is key to counteracting oxidative stress[J].Journal of Biology,2007,6(4):1.
• [71]李银萍,牛俊奇.慢性丙型肝炎病毒感染及抗病毒治疗与胰岛β细胞功能的关系[J].中华传染病杂志,2014,32(3).
• [72]刘含亮.注射葡萄糖对罗非鱼糖代谢、胰岛素受体和GLUT4基因表达的影响[D].山东农业大学,2012.