黄一伟;李梓;张红;刘运芝;温乐英;李俊华;高立冬;赵新生;李中杰;陈碧云;蓝雨;周蕾;舒跃龙;

• 1:湖南省疾病预防控制中心
• 2:中国疾病预防控制中心病毒病预防控制所
• 3:湖南省疾病预防控制中心
• 4:湖南省疾病预防控制中心

摘要(Abstract)：

为了对2005²006年湖南省2例不明原因肺炎病例进行实验室诊断,确定病因以及对其进行病原学研究,采集病例呼吸道标本和血清标本,对呼吸道标本采用实时荧光定量逆转录聚合酶链式反应(Real-time RT-PCR)和逆转录聚合酶链式反应(RT-PCR)方法检测H5亚型禽流感病毒核酸,对血清标本采用血凝抑制试验检测特异性抗体,并对其中1例死亡病例(病例2)的肺穿刺物标本进行病毒分离,所获毒株予以测序及同源性分析。结果显示,2例病例H5亚型禽流感病毒核酸检测均为阳性,病例1恢复期血清H5N1特异性抗体阳性,并且较急性期血清呈4倍以上增长;病例2急性期血清特异性抗体阴性,2例均为人感染高致病性禽流感病毒(H5N1)确诊病例。从病例2分离得到毒株A/Hunan/1/2006,测序及分子特性分析表明,其8个基因片段均为禽源,且与湖南本地禽类分离的病毒相似,并未与人流感病毒发生基因重组或产生显著变异。

关键词(KeyWords)： H5N1禽流感;实验室诊断;基因特性

Abstract:

Keywords:

基金项目(Foundation):

作者(Authors): 黄一伟;李梓;张红;刘运芝;温乐英;李俊华;高立冬;赵新生;李中杰;陈碧云;蓝雨;周蕾;舒跃龙;

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