曾怡,卢春

• 1:南京医科大学微生物学与免疫学系
• 2:南京医科大学微生物学与免疫学系 南京 210029
• 3:南京 210029

摘要(Abstract)：

运用细胞融合、细胞混合培养、条件培养基培养和病毒直接刺激等方法,研究人类疱疹病毒6型(HHV6)对卡波济肉瘤相关疱疹病毒(KSHV)溶解性周期复制的影响。①将HHV6感染的JJhan细胞(T淋巴细胞系)与BCBL-1细胞(原发性渗出性淋巴瘤,PEL)进行细胞融合形成异核体细胞。②将HHV6感染的JJhan细胞与BCBL-1细胞进行混合培养。③收集HHV6感染的JJhan细胞培养上清液作为条件培养基进行灭活处理,以灭活前后的条件培养基培养BCBL-1细胞。进一步离心纯化HHV6病毒颗粒,并感染BCBL-1细胞,分别设紫外线和热灭活的HHV6病毒颗粒感染BCBL-1细胞为对照。提取上述的实验细胞总RNA,RT-PCR和/或实时定量(Real—time)PCR检测卡波济肉瘤相关疱疹病毒(KSHV)次要衣壳蛋白编码基因ORF26mRNA转录。结果显示:①细胞融合后15h开始出现明显细胞病变,RT-PCR检测不同时间的实验组ORF26mRNA转录水平均明显高于对照组;Real—timePCR检测各时间ORF26mRNA转录水平是对照组的2.3倍以上;②细胞混合培养72h时,实验组ORF26mRNA转录水平是对照组的1.8倍;混合培养5天时,实验组KSHV裂解周期蛋白K8.1表达水平是对照组的2.46倍;③灭活前后的HHV6感染细胞培养上清液培养BCBL-1细胞96h时,ORF26mRNA转录水平分别是对照组的2.73倍和2.22倍;④灭活前后的HHV6均可增强BCBL-1细胞中KSHVORF26mRNA转录水平。提示:HHV6感染可激活KSHV的溶解性周期复制。

关键词(KeyWords)： 人类疱疹病毒6型(HHV6);卡波济肉瘤相关疱疹病毒(KSHV);溶解性周期复制

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金项目(30100160和30271179)

作者(Author): 曾怡,卢春

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