重组细胞高产型H3N2猪流感病毒株的拯救Generation of Cell Culture High-yield Recombinant H3N2 Subtype Swine Influenza Vaccine Candidate by Reverse Genetics
杨涛;刘明;刘春国;张云;刘大飞;陈浩;童光志;
摘要(Abstract)：
为拯救出一株能够在动物传代细胞中高水平复制的H3N2亚型猪流感疫苗株,利用反向遗传操作技术,将A/Goose/Dalian/3/01(H9N2)毒株的PB1、PA、NP、M、NS基因和A/PR/8/34毒株的PB2基因作为内部基因与猪流感病毒A/Swine/Henan/S4/01(H3N2)毒株的HA、NA基因进行重组,成功拯救出了具有高度细胞适应性毒株rH3N2株,该毒株接毒MDCK细胞60h后,血凝价可以达到1∶512,表明该毒株具有高度适应细胞繁殖特性,为H3N2亚型猪流感病毒细胞培养型疫苗的研制奠定了基础。
关键词(KeyWords)： 猪流感;H3N2亚型;反向遗传操作技术;细胞培养型流感疫苗
基金项目(Foundation): 国家“973”计划资助项目(2005CB523200);; 国家“十一五”科技支撑计划资助项目(2006BAD06A05)
作者(Authors): 杨涛;刘明;刘春国;张云;刘大飞;陈浩;童光志;
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