雷雯雯,郭晓芳,付士红,冯云,宋敬东,周红宁,梁国栋

• 1:中国疾病预防控制中心病毒病预防控制所传染病预防控制国家重点实验室
• 2:云南省寄生虫病防治所云南省虫媒传染病防控研究重点实验室
• 3:云南省地方病防治所云南省病毒立克次体研究中心

摘要(Abstract)：

2012年在云南省南部中老缅边境地区采集的库蚊中分离到3株病毒(YN12039、YN12040、YN12060),该病毒只引起C6/36细胞发生病变。负染电镜观察病毒颗粒呈球形,直径60~80nm,有囊膜,表面有刺突。采用RTPCR方法对分离株的RNA依赖的RNA聚合酶(RNA-dependent RNA-polymerase,RdRp)、HEL1(superfamily 1helicase)、S蛋白(spike protein)基因进行扩增,同源性分析显示3株云南新分离株与Nam Dinh病毒(NDiV)RdRp、HEL1、S蛋白氨基酸序列相似度最高,均为98%以上。系统进化分析结果表明,3株云南新分离株与NDiV的亲缘关系最近,处于同一进化分支。该研究结果丰富了NDiV病毒的信息,对NDiV病毒分子特征、分布区域和物种嗜性等特征提供了数据参考。

关键词(KeyWords)： Nam Dinh病毒;RNA依赖的RNA聚合酶(RdRp);HEL1基因;S基因

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金(项目号:81290342),题目:蚊虫携带人类病原体的发现与分离鉴定、分布特点、遗传进化规律及其与人类疾病的关系;; 传染病预防控制国家重点实验室项目(项目号:2014SKLID103),题目:我国新分离双链RNA病毒全基因组遗传进化特征研究;; 国家重点研发计划(项目号:2016YFD0500300),题目:重大突发动物源性人兽共患病跨种感染与传播机制研究

作者(Author): 雷雯雯,郭晓芳,付士红,冯云,宋敬东,周红宁,梁国栋

DOI: 10.13242/j.cnki.bingduxuebao.003077

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