山东省柯萨奇病毒B1型VP1区全基因序列分析Phylogenetic Analysis of Complete VP1 Sequence of Coxsackievirus B1 in Shandong Province,China
王海岩,陶泽新,李岩,张文强,刘晓林,徐爱强
摘要(Abstract):
为了解柯萨奇病毒B1型(Coxsackievirus B1,CV-B1)山东地方株的分子流行病学特征,本研究对1994年至2015年山东省急性弛缓性麻痹(Acute flaccid paralysis,AFP)监测系统、环境污水监测和无菌性脑膜炎病例标本中分离到的CV-B1病毒进行了VP1序列测定、系统发生学分析和同源性分析。共分离到CV-B1病毒53株,其中AFP监测、污水和及脑炎标本各分离到41株、4株和8株。基于VP1完整编码区序列的系统发生学分析显示CVB1山东株与国内其他分离株属于一个大的分支,该分支内无国外分离株,国外分离株构成了其他两个分支。山东株之间的VP1核苷酸同源性为84.4%~100.0%,与其他国家分离株的同源性为77.9%~85.0%。研究结果表明,中国CV-B1分离株与国外株相比有较大的遗传差异,需要加强相关手足口病病毒学监测,关注不同传播链的新的基因亚型的肠道病毒的输入。
关键词(KeyWords): 手足口病;柯萨奇病毒B1型;系统发生;肠道病毒;同源性
基金项目(Foundation): 泰山学者工程专项经费资助(项目号:ts.201511105);; 山东省自然科学基金项目(项目号:ZR2012HM007),题目:柯萨奇病毒B病毒体外重组感染性克隆的构建及生物学特性的鉴定;; 国家自然科学基金项目(项目号:81302481),题目:环境污水中人类肠胃炎病毒的型别多样性、遗传进化规律及其与人群疾病发生的关系
作者(Author): 王海岩,陶泽新,李岩,张文强,刘晓林,徐爱强
DOI: 10.13242/j.cnki.bingduxuebao.003062
参考文献(References):
- [1]Pallansch M A,Oberste M S,Whitton J L.2013.Enteroviruses:polioviruses,coxsackieviruses,echoviruses,and newer enteroviruses,p 490–530.In Knipe DM,Howley PM(ed),Fields Virology[M].6th ed,vol 1.Lippincott Williams&Wilkins,Philadelphia,PA.
- [2]Khetsuriani N,Lamonte-Fowlkes A,Oberst S,Pallansch M A;Centers for Disease Control and Prevention.Enterovirus surveillance-United States,1970-2005[J].MMWR Surveill Summ,2006,55(8):1-20.
- [3]Laitinen O H,Honkanen H,Pakkanen O,Oikarinen S,Hankaniemi M M,Huhtala H,Ruokoranta T.Coxsackievirus B1is associated with induction of b-cell autoimmunity that portends type 1diabetes[J].Diabetes,2014,63(2):446-455.
- [4]Centers for Disease Control and Prevention(CDC).Nonpolio Enterovirus and Human Parechovirus Surveillance-United States,2006-2008[J].MMWR Morb Mortal Wkly Rep,2010,59(48):1577-1580.
- [5]Abedi G R,Watson J T,Pham H,Nix W A,Oberste M S,Gerber S I.Enterovirus and human parechovirus surveillance-United States,2009-2013[J].MMWR Morb Mortal Wkly Rep,2015,64(34):940-943.
- [6]Wikswo M E,Khetsuriani N,Fowlkes A L,Zheng X,Penaranda S,Verma N,Shulman S T,Sircar K,Robinson C C,Schmidt T,Schnurr D,Oberste M S.Increased activity of Coxsackievirus B1strains associated with severe disease among young infants in the United States,2007–2008[J/OL].Clin Infect Dis,2009,49(5):e44-e51.
- [7]Bingjun T,Yoshida H,Yan W,Lin L,Tsuji T,Shimizu H,Miyamura T.Molecular typing and epidemiology of non-polio enteroviruses isolated from Yunnan Province,the People's Republic of China[J/OL].J Med Virol,2008,80(4):670-679.
- [8]汤晶晶,赵智娴,田炳均,罗梅,张杰,丁峥嵘.云南省2006~2010年非脊灰肠道病毒分子流行病学特征分析[J].病毒学报,2013,29(2):169-175.
- [9]王海岩,刘坤,陶泽新,李岩,刘桂芳,刘尧,冯蕾,樊庆莹,杨赫,宋立志,徐爱强.肠道病毒B组山东地方株的基因型分布[J].病毒学报,2010,26(5):357-362.
- [10]WHO.2004.Polio laboratory manual,4th ed[M].Geneva,Switzerland.
- [11]Tao Z,Zhang Y,Liu Y,Xu A,Lin X,Yoshida H,Xiong P,Zhu S,Wang S,Yan D,Song L,Wang H,Cui N,Xu W.Isolation and characterization of a type2vaccine-derived poliovirus from environmental surveillance in China,2012[J/OL].PLoS One,2013,8(12):e83975.
- [12]Wang H,Tao Z,Li Y,Lin X,Yoshida H,Song L,Zhang Y,Wang S,Cui N,Xu W,Song Y,Xu A.Environmental surveillance of human enteroviruses in Shandong Province,China,2008to 2012:serotypes,temporal fluctuation,and molecular epidemiology[J].Appl Environ Microbiol,2014,80(15):4683-4691.
- [13]Oberste M S,Maher K,Williams A J,Dybdahl-Sissoko N,Brown B A,Gookin M S,Pearanda S,Mishrik N,Uddin M,Pallansch M A.Species-specific RTPCR amplification of human enteroviruses:a tool for rapid species identification of uncharacterized enteroviruses[J].J Gen Virol,2006,87(1):119-128.
- [14]Oberste M S,Mather K,Kilpatrick D R,Pallansch M A.Molecular evolution of the human enteroviruses:correlation of serotype with VP1sequence and application to picornavirus classification[J].J Virol,1999,73(3):1941-1948.
- [15]Abdelkhalek I,Seghier M,Yahia A B,Touzi H,Meddeb Z,Triki H,Rezig D.Molecular epidemiology of coxsackievirus type B1[J].Arch Virol,2015,160:2815-2821.
- [16]Kim H J,Kang B,Hwang S,Hong J,Chung J,Kim S,Jeong Y S,Kim K,Cheon D S.Molecular characteristics of human coxsackievirus B1infection in Korea,2008-2009.J Med Virol,2013,85:110-115.
- [17]Chu P Y,Tyan Y C,Chen Y S,Chen H L,Lu P L,Chen Y H,Chen B C,Huang T S,Wang C F,Su H J,Shi Y Y,Sanno-Duanda B,Lin K H,Motomura H.Transmission and demographic dynamics of coxsackievirus B1[J/OL].PLoS One,2015,10(6):e0129272.
- [18]Tao Z,Song Y,Li Y,Liu Y,Jiang P,Lin X,Liu G,Song L,Wang H,Xu A.Coxsackievirus B3,Shandong Province,China,1990-2010[J].Emerg Infect Dis,2012,18(11):1865-1867.