用于狂犬病单克隆抗体中和活性检测病毒库的制备及初步应用Preparation and Preliminary Application of a Virus Library for the Neutralizing Activity of a Monoclonal Antibody Against the Rabies Virus
于鹏程,陶晓燕,王力华,唐青,刘淑清,朱武洋
摘要(Abstract):
以狂犬病街毒株为攻击病毒建立检测狂犬病单克隆抗体中和活性的方法。小鼠脑内接种分离扩增狂犬病阳性标本24株;分离到的街毒株在N2A细胞进行培养及病毒滴度测定,选择能够适应N2A细胞生长且滴度较高的街毒株建立病毒库;建立方法后对TRN006单克隆抗体进行3次检测,评价其重复性。24份狂犬病阳性标本均分离成功;只有15株病毒能够适应N2A细胞,并在细胞上形成荧光灶;最终选取10株滴度较高病毒建立病毒库,该病毒库覆盖了中国9个不同省份及四个中国毒株群;用其中三种病毒对狂犬病单克隆抗体进行中和活性检测3次,T检验发现其具有良好的重复性。
关键词(KeyWords): 狂犬病;单克隆抗体;中和抗体
基金项目(Foundation): 国家重点研发计划项目(2016YFD0500400),题目:重要神经嗜性人兽共患病免疫与致病机制研究;; 国家科技重大专项项目(2014ZX10004002),题目:艾滋病和病毒性肝炎等重大传染病防治;; 国家科技支撑计划(2014BAI13B04),题目:狂犬病净化技术集成与人狂犬病消除示范研究;; 国家自然科学基金(31500152),题目:我国狂犬病病毒种群划分系统的建立
作者(Author): 于鹏程,陶晓燕,王力华,唐青,刘淑清,朱武洋
DOI: 10.13242/j.cnki.bingduxuebao.003078
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