严伟东;杨郑欣;马宁宁;张梦佳;邵伟;何启盖;李文涛;

• 1:华中农业大学农业微生物学国家重点实验室
• 2:武汉科前生物股份有限公司
• 3:华中农业大学动物科学技术学院动物医学院
• 4:生猪健康养殖协同创新中心

摘要(Abstract)：

盖塔病毒（Getah virus,GETV）是一种人畜共患病毒，近年来在动物中的感染时有发生，监测其流行情况以及控制其传播对公共卫生方面至关重要。本研究对一猪场流产胎儿脾脏样品进行二代测序，与NCBI数据库比对后，发现该序列与GETV HNJZ-S2毒株的基因组高度相似。对组织样品进行无菌处理，接种至PK-15细胞，经连续传代培养和空斑纯化，获得一株能在PK-15细胞上稳定生长的分离物，命名为GETV-YL株，并对其进行电镜观察与遗传演化分析。电镜结果显示，病毒颗粒呈球状，直径约为70 nm，有囊膜，表面有甲病毒典型的纤突结构。遗传演化分析结果显示，GETV-YL基因组全长11 689bp，与GenBank中现有的30株GETV全基因组序列相似性为94.4%～99.8%，与中国猪源毒株HNJZ-S2序列相似性最高（99.8%）并处于同一分支。在本研究中，从感染的猪中检测并分离到了GETV，表明该地区应加强对该病毒在猪群中流行情况的监测，并对该地区的蚊子进行病原检测。

关键词(KeyWords)： 猪;盖塔病毒;分离鉴定;遗传演化分析

Abstract:

Keywords:

基金项目(Foundation): 财政部和农业农村部：国家现代农业产业技术体系资助（项目号：CARS-35）

作者(Authors): 严伟东;杨郑欣;马宁宁;张梦佳;邵伟;何启盖;李文涛;

DOI: 10.13242/j.cnki.bingduxuebao.004228

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