李庆军,张波,张绮玲,王鑫,霍雨佳,杨静

• 1:军事医学科学院放射与辐射医学研究所
• 2:河南中医药大学药学院
• 3:北京协和医院药剂科

摘要(Abstract)：

研究磷脂爬行酶1(Phospholipid scramblase 1,PLSCR1)对干扰素抑制HBV作用的影响。设计合成PLSCR1特异性小干扰RNA(siRNA),以完全随机序列的阴性小干扰(NCsiRNA)作为对照,转染HepG2细胞,于转染48h后分别检测PLSCR1mRNA和蛋白水平表达量的变化,筛选出对PLSCR1具有沉默作用的siRNA;将HepG2细胞分为正常对照组和干扰素处理组,将1.3倍乙型肝炎病毒(HBV)全基因真核细胞表达载体HBV1.3质粒分别与PLSCR1siRNA或NCsiRNA共同转染HepG2细胞或干扰素处理的HepG2细胞,转染48h后检测各组细胞中PLSCR1mRNA表达量及培养液上清中HBsAg表达水平。PLSCR1特异性小干扰RNA siRNA911转染后能够显著抑制HepG2细胞中PLSCR1基因在mRNA和蛋白水平的表达;与HepG2细胞对照组比较,干扰素处理组细胞转染HBV1.3质粒、NCsiRNA+HBV1.3质粒后,细胞培养液中HBsAg表达水平均显著降低(P<0.05);而PLSCR1siRNA与HBV1.3共转染IFN处理的HepG2细胞组与共转染HepG2细胞组相比较,细胞培养液中HBsAg的表达水平没有显著差异。提示抑制PLSCR1的siRNA可抑制干扰素的抗HBV活性,提示PLSCR1在干扰素抑制HBV复制中具有重要作用。

关键词(KeyWords)： 乙型肝炎病毒(HBV);磷脂爬行酶1(PLSCR1);乙型肝炎病毒;干扰素

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金课题(项目号:31270197),题目:磷脂爬行酶1抑制HBV复制的分子机制研究

作者(Author): 李庆军,张波,张绮玲,王鑫,霍雨佳,杨静

DOI: 10.13242/j.cnki.bingduxuebao.003072

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