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本研究针对2024年6月北京经济技术开发区发生的两起聚集性发热疫情，开展了病原学鉴定和基因特征分析，旨在为该类疫情的防控提供实验室依据。研究采集了两起发热疫情中6例病例的咽拭子标本，采用实时荧光定量RT-PCR方法进行呼吸道多种病原体的核酸筛查，结果显示6份标本均为肠道病毒核酸阳性。进一步通过病毒分离、分型鉴定和VP1区进化分析，确认两起疫情均是由柯萨奇病毒A组4型（Coxsackievirus A4, CVA4）感染引起，且均属于C基因型。这一结果表明，应加强肠道病毒尤其是CVA4引起的聚集性发热疫情的监测和防控工作。
Abstract：The etiological identification and genetic characteristics of two clustered fever epidemics in Beijing Economic-Technological Development Area were analyzed, aiming to provide a laboratory basis for the prevention and control of such epidemics. Throat swab specimens were collected from 6 cases in two clustered fever epidemics, and real-time quantitative RT-PCR was performed to detect multiple respiratory pathogens. Results showed that all 6 samples were positive for enterovirus nucleic acid. Further virus isolation, typing, and VP1 region evolutionary analysis confirmed that both outbreaks were caused by Coxsackievirus group A(CVA4) infection, and both belonged to the C genotype. These results suggest that monitoring, prevention and control of cluster fever outbreaks caused by enteroviruses, especially CVA4, should be strengthened.
[1] Xie Z, Khamrin P, Maneekarn N, et al. Epidemiology of enterovirus genotypes in association with human diseases[J].Viruses, 2024, 16(7):1165.DOI:10.3390/v16071165.
[2] Ji T, Guo Y, Lv L, et al. Emerging recombination of the C2 sub-genotype of HFMD-associated CV-A4 is persistently and extensively circulating in China[J].Sci Rep, 2019, 9(1):13668.DOI:10.1038/s41598-019-49859-7.
[3]孙振璐，徐迎春，刘娟，等.2015年烟台地区手足口病监测中柯萨奇病毒A4基因特征分析[J].病毒学报，2018, 34(4):471-476.DOI:10.13242/j.cnki.bingduxuebao.003397.
[4] Guo WP, Chen GQ, Xie GC, et al. Mosaic genome of human coxsackievirus A4 associated with herpangina and HFMD in Yancheng, China, 2016 and 2018[J].Int J Infect Dis, 2020, 96:538-540.DOI:10.1016/j.ijid.2020.05.057.
[5] Li JS, Dong XG, Qin M, et al. Outbreak of febrile illness caused by coxsackievirus A4 in a nursery school in Beijing, China[J].Virol J, 2015, 12:92.DOI:10.1186/s12985-015-0325-1.
[6]邹林，甄博珺，张萍，等.北京市通州区一起聚集性发热疫情的病原学鉴定及柯萨奇病毒A4型基因特征分析[J].病毒学报，2024, 40(06):1316-1323.DOI:10.13242/j.cnki.bingduxuebao.004572.
[7]袁芳，曹慜，马学平，等.2017—2021年宁夏急性驰缓性麻痹病例监测系统非脊灰肠道病毒病原鉴定分析[J].病毒学报，2023, 39(2):460-467.DOI:10.13242/j.cnki.bingduxuebao.004295.
[8] Mine I, Taguchi M, Sakurai Y, et al. Bilateral idiopathic retinal vasculitis following coxsackievirus A4 infection:a case report[J].BMC Ophthalmol, 2017, 17(1):128.DOI:10.1186/s12886-017-0523-2.
[9] Drago F, Ciccarese G, Gariazzo L, et al. Acute localized exanthem due to coxsackievirus A4[J].Infez Med, 2017, 25(3):274-276.
[10]Jartti M, Flodstr?m-Tullberg M, Hankaniemi MM.Enteroviruses:epidemic potential, challenges and opportunities with vaccines[J].J Biomed Sci, 2024, 31(1):73.DOI:10.1186/s12929-024-01058-x.
[11]WHO.Polio Laboratory Manual[S].World Health Organization, The 4th edition, Geneva, 2004;81-100
[12]Steven Oberste M, Maher K, Williams AJ, et al. Species-specific RT-PCR amplification of human enteroviruses:a tool for rapid species identification of uncharacterized enteroviruses[J].J Gen Virol, 2006, 87(Pt 1):119-128.DOI:10.1099/vir.0.81179-0.
[13]蔡剑，凌锋，丰燕，等.浙江省2013—2019年发热疫情的病原学和流行病学特征分析[J].实用预防医学，2022, 29(7):836-839.
[14]Melnick JL.Studies on the Coxsackie viruses;properties, immunological aspects and distribution in nature[J].Bull N Y Acad Med, 1950, 26(5):342-356.
[15]吉彦莉，王永全，崔海洋，等.一起由柯萨奇病毒A4引起的聚集性发热的分子流行病学研究[J].公共卫生与预防医学，2019, 30(2):93-96.DOI:10.3969/j.issn.1006-2483.2019.02.023.
[16]张洁，游春芳，明雪微，等.2023年自贡市一起柯萨奇病毒A4引起的幼儿园疱疹性咽峡炎暴发疫情[J].职业卫生与病伤，2024, 39(1):21-25.DOI:10.19973/j.cnki.1006-172X.23.103.
[17]Li J, Ni N, Cui Y, et al. An outbreak of a novel recombinant Coxsackievirus A4 in a kindergarten,Shandong province, China, 2021[J].Emerg Microbes Infect, 2022, 11(1):2207-2210.DOI:10.1080/22221751.2022.2114855.
[18]Oberste MS, Maher K, Kilpatrick DR, et al. Typing of human enteroviruses by partial sequencing of VP1[J].J Clin Microbiol, 1999, 37(5):1288-1293.DOI:10.1128/JCM.37.5.1288-1293.1999.
[19]Chu PY, Lu PL, Tsai YL, et al. Spatiotemporal phylogenetic analysis and molecular characterization of coxsackievirus A4[J].Infect Genet Evol, 2011, 11(6):1426-1435.DOI:10.1016/j.meegid.2011.05.010.
[20]Chen P, Wang H, Tao Z, et al. Multiple transmission chains of coxsackievirus A4 co-circulating in China and neighboring countries in recent years:Phylogenetic and spatiotemporal analyses based on virological surveillance[J].Mol Phylogenet Evol, 2018, 118:23-31.DOI:10.1016/j.ympev.2017.09.014.
[21]陈诹，王远萍，徐红梅，等.2013-2015年上海市浦东新区聚集性发热疫情分析[J].实用预防医学，2017,24(5):563-565.DOI:10.3969/j.issn.1006-3110.2017.05.014.
基本信息:
DOI：10.13242/j.cnki.bingduxuebao.250117
中图分类号:R373
引用信息:
[1]尉秀霞,肖金波,李敏,等.聚集性发热疫情相关的柯萨奇病毒A组4型VP1区基因特征分析[J].病毒学报,2025,41(05):1361-1366.DOI:10.13242/j.cnki.bingduxuebao.250117.
基金信息:
北京市自然科学基金-昌平创新联合基金项目(项目号：L234052),题目：我国手足口病病原谱及重要肠道病毒变异变迁规律~~