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了解甘肃省前3例猴痘病例流行病学特征和基因组特征及遗传变异情况，为甘肃省猴痘的防控提供科学依据。对甘肃省报告的前3例猴痘病例开展流行病学调查并采集相应样本，利用Illumina mini-seq二代测序仪完成猴痘病毒全基因组测序和数据处理，采用生物信息学软件对病毒全基因组序列进行遗传特征、谱系分型和进化树溯源分析。甘肃省报告的前3例猴痘病例均症状较轻，主要为疱疹伴瘙痒和神经疼痛，无发热等全身症状，主要传播途径为男男性行为密切接触，但使用安全套可能无法有效阻断传播；测序获得的全基因组序列全长分别为病例1(GanSu-JC)197 199bp；病例2(GanSu-LZ)197 200bp；病例3(GanSu-BY)197 199bp，基因组覆盖度分别为97.12%、99.37%、99.38%，与参考序列（NC＿063383.1）相比3例病例猴痘病毒基因组序列分别存在78、88、87处核苷酸突变，分别有38、42、42个氨基酸发生突变，涉及宿主免疫调控、病毒致病性等生物特性发生改变；系统遗传进化分析显示，GanSu-JC序列与韩国提交的序列遗传进化亲缘关系较近；GanSu-LZ和GanSu-BY序列与中国深圳、云南提交的序列遗传进化亲缘关系较近，均属于Ⅱb C.1谱系。甘肃省报告的前3例猴痘病例均为西非亚型Ⅱb C.1谱系，兰州市2023年底之前很可能已存在猴痘病例，提示甘肃应加强猴痘防控宣教、鉴别诊断和防治工作。
Abstract：To understand the epidemiological features, genomic characteristics and genetic variations of the first three cases of monkeypox in Gansu Province, China, so as to provide a scientific basis for the prevention and control of monkeypox. The first three cases of monkeypox reported in Gansu Province, China were investigated epidemically and samples were collected. The whole genome sequencing and data processing of monkeypox virus were performed with Illumina mini-seq next-generation sequencer, and the genetic characteristics, lineage typing and evolutionary tree traceability analysis of the whole genome sequence of the virus were carried out with bioinformatics software. The first three cases of monkeypox reported in Gansu Province, China were all mild, mainly herpes with itching and nerve pain, without systemic symptoms such as fever. The main transmission route was close contact between men and men, and the use of condoms may not be effective in blocking transmission. The full length of the virus whole genome sequence obtained was 197199 bp for case 1(GanSu-JC). Case 2(GanSu-LZ) was 197,200 bp. Case 3(GanSu-BY) was 197199 bp. The genome coverage was 97.12%, 99.37%, and 99.38%, respectively, compared with the reference sequence(NC＿063383.1). There were 78, 88, and 87 nucleotide mutations in the genome sequence of monkeypox virus in the three cases, and 38, 42, and 42 amino acids were mutated, respectively, involving changes in host immune regulation, virus pathogenicity and other biological characteristics. Phylogenetic analysis showed that the GanSuJC sequence was closely related to the sequence submitted by Korea. The GanSu-LZ and GanSu-BY are closely related to the genetic and evolutionary relatives of the sequences submitted by Shenzhen and Yunnan in China, and both belong to the II.b C.1 lineage. The first three monkeypox cases reported in Gansu Province, China were all of the West African subtype II. b C. 1 lineage, and it is likely that there were monkeypox cases in Lanzhou City before the end of 2023, suggesting that Gansu, China should strengthen the education, differential diagnosis and prevention of monkeypox.
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基本信息:
DOI：10.13242/j.cnki.bingduxuebao.240122
中图分类号:R181.3;R511
引用信息:
[1]张慧,王彪,张晓曙,等.甘肃省前3例猴痘病例流行病学特点和基因组序列特征分析[J].病毒学报,2025,41(02):354-360.DOI:10.13242/j.cnki.bingduxuebao.240122.
基金信息:
甘肃省青年基金科技计划项目（项目号：20JR5RA141）,题目：下呼吸道病原与NETs标志物相关性研究; 甘肃省自然科学基金（项目号：20JR5RA142）,题目：甘肃省疱疹性咽峡炎病原学监测研究~~