2024 05 v.40 978-985
诺如病毒GⅡ.4 IgG抗体的荧光素酶免疫吸附方法的建立及在动物血清中的应用
基金项目(Foundation):
广东省重点领域研发项目（项目号：2022B1111020002）,题目：华南地区主要流行人类病毒模式小鼠资源库的建立及评估应用;;
国家自然科学基金（项目号：31771007）,题目：GII.4型诺如病毒全人广谱中和单抗及其保守表位研究;;
广东省自然科学基金（项目号：2019A1515010951）,题目：基于自然人群队列诺如病毒基因型更迭的群体免疫机制研究~~
邮箱(Email):
qch.2009@163.com;yingchun78@hot-mail.com;
DOI:
10.13242/j.cnki.bingduxuebao.004582
中文作者单位:
南方医科大学公共卫生学院;
摘要(Abstract):
诺如病毒（Norovirus, NoV）是引起全球范围内人类急性胃肠炎（Acutegastroenteritis, AGE）的主要病原体，对全球健康和经济造成了重大威胁。已有研究在不同种类的动物粪便中检出人类诺如病毒（Human norovirus,HuNoV）的核酸或在家畜血清中检测出可识别HuNoV病毒样颗粒（Virus like particles,VLP）的抗体，这引起了公众对NoV是否为潜在人兽共患病的关注与讨论。本研究基于HuNoV主要流行株GⅡ.4建立了一种检测GⅡ.4IgG的荧光素酶免疫吸附试验（Luciferase immunosorbent assay,LISA）。LISA法具有较高的灵敏度（94.87%）和特异度（98.18%）；最低检测限是ELISA法的2～16倍；GⅡ.4-LISA检测人血清的批间和批内变异系数值均小于10%，重复性良好。GⅡ.4 IgG在狗、猪、蝙蝠、大鼠和鼩鼱等不同的家畜和野生动物中均有检出，阳性率在3.37%～39.53%之间，其中蝙蝠的检出率最高（39.53%），狗次之（9.72%）。综上所述，本研究建立的GⅡ.4-LISA方法适用于人和不同种属动物中GⅡ.4特异性IgG检测。针对HuNoV的IgG抗体广泛分布，提示NoV可能是潜在的人畜共患病。
关键词(KeyWords):
诺如病毒GⅡ.4;;荧光素酶免疫吸附法;;IgG抗体;;家畜和野生动物
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参考文献
[1] Thorne LG, Goodfellow IG. Norovirus gene expression and replication[J]. J Gen Virol, 2014, 95(pt 2):278-291. DOI:10. 1099/vir. 0. 059634-0.
[2] VinjéJ. Advances in laboratory methods for detection and typing of norovirus[J]. J Clin Microbiol, 2015, 53(2):373-381. DOI:10. 1128/jcm. 01535-14.
[3] Wang QH, Han MG, Cheetham S, et al. Porcine noroviruses related to human noroviruses[J]. Emerg Infect Dis, 2005, 11(12):1874-1881. DOI:10. 3201/eid1112. 050485.
[4] Chhabra P, de Graaf M, Parra GI, et al. Updated classification of norovirus genogroups and genotypes[J].J Gen Virol, 2019, 100(10):1393-1406. DOI:10. 1099/jgv. 0. 001318.
[5] de Graaf M, van Beek J, Koopmans MPG. Human norovirus transmission and evolution in a changing world[J]. Nat Rev Microbiol, 2016, 14:421-433. DOI:10. 1038/nrmicro. 2016. 48.
[6] Yoon HJ, Hur SH. Determination of safe contrast media dosage to estimated glomerular filtration rate ratios to avoid contrast-induced nephropathy after elective percutaneous coronary intervention[J]. Korean Circ J, 2011, 41(5):265-271. DOI:10. 4070/kcj. 2011. 41. 5. 265.
[7] Ramani S, Atmar RL, Estes MK. Epidemiology of human noroviruses and updates on vaccine development[J]. Curr Opin Gastroenterol, 2014, 30(1):25-33.DOI:10. 1097/mog. 0000000000000022.
[8] Patel MM, Widdowson MA, Glass RI, et al.Systematic literature review of role of noroviruses in sporadic gastroenteritis[J]. Emerg Infect Dis, 2008, 14(8):1224-1231. DOI:10. 3201/eid1408. 071114.
[9] Netzler NE, Enosi Tuipulotu D, White PA. Norovirus antivirals:Where are we now?[J]. Med Res Rev,2019, 39(3):860-886. DOI:10. 1002/med. 21545.
[10]Widdowson MA, Rockx B, Schepp R, et al. Detection of serum antibodies to bovine norovirus in veterinarians and the general population in the Netherlands[J]. J Med Virol, 2005, 76(1):119-128. DOI:10. 1002/jmv. 20333.
[11]Mesquita JR, Costantini VP, Cannon JL, et al.Presence of antibodies against genogroup VI norovirus in humans[J]. Virol J, 2013, 10:176. DOI:10. 1186/1743-422x-10-176.
[12]Humphrey TJ, Cruickshank JG, Cubitt WD. An outbreak of calicivirus associated gastroenteritis in an elderly persons home. A possible zoonosis?[J]. J Hyg(Lond), 1984, 93(2):293-299. DOI:10. 1017/s0022172400064822.
[13]Summa M, von Bonsdorff CH, Maunula L. Pet dogs—a transmission route for human noroviruses?[J]. J Clin Virol, 2012, 53(3):244-247. DOI:10. 1016/j.jcv. 2011. 12. 014.
[14]Caddy SL, de Rougemont A, Emmott E, et al.Evidence for human norovirus infection of dogs in the United Kingdom[J]. J Clin Microbiol, 2015, 53(6):1873-1883. DOI:10. 1128/jcm. 02778-14.
[15]Nakamura K, Saga Y, Iwai M, et al. Frequent detection of noroviruses and sapoviruses in swine and high genetic diversity of porcine sapovirus in Japan during Fiscal Year 2008[J]. J Clin Microbiol, 2010, 48(4):1215-1222. DOI:10. 1128/jcm. 02130-09.
[16]Bucardo F, González F, Reyes Y, et al.Seroprevalence in household raised pigs indicate high exposure to GII noroviruses in rural Nicaragua[J].Zoonoses Public Health, 2016, 63(8):600-607. DOI:10. 1111/zph. 12269.
[17]Liang Z, Zhang M, Wang Y, et al. Development and evaluation of a new luciferase immunosorbent assay to detect GII. 6 norovirus-specific IgG in different domestic and wild animals[J]. Front Microbiol, 2023, 14:1213007. DOI:10. 3389/fmicb. 2023. 1213007.
[18]Wang H, Cai Q, Liang Y, et al. A simple and highthroughput luciferase immunosorbent assay for both qualitative and semi-quantitative detection of anti-HIV-1antibodies[J]. Virus Res, 2019, 263:9-15. DOI:10. 1016/j. virusres. 2018. 12. 017.
[19]Bank-Wolf BR, K?nig M, Thiel HJ. Zoonotic aspects of infections with noroviruses and sapoviruses[J]. Vet Microbiol, 2010, 140(3-4):204-212. DOI:10. 1016/j. vetmic. 2009. 08. 021.
[20]王天禹，芜为，邓瑶，等.基于寨卡病毒NS2B蛋白特异抗原多肽的荧光素酶免疫吸附法检测寨卡病毒抗体[J].病毒学报，2019, 35(3):396-401. DOI:10. 13242/j. cnki. bingduxuebao. 003546.
[21]Hall MP, Unch J, Binkowski BF, et al. Engineered luciferase reporter from a deep sea shrimp utilizing a novel imidazopyrazinone substrate[J]. ACS Chem Biol,2012, 7(11):1848-1857. DOI:10. 1021/cb3002478.
[22]Baeshen MN, Al-Hejin AM, Bora RS, et al.Production of biopharmaceuticals in E. coli:current scenario and future perspectives[J]. J Microbiol Biotechnol, 2015, 25(7):953-962. DOI:10. 4014/jmb. 1412. 12079.
[23]Wolf S, Reetz J, Johne R, et al. The simultaneous occurrence of human norovirus and hepatitis E virus in a Norway rat(Rattus norvegicus)[J]. Arch Virol, 2013,158(7):1575-1578. DOI:10. 1007/s00705-013-1646-2.
[24]Kocher JF, Lindesmith LC, Debbink K, et al. Bat caliciviruses and human noroviruses are antigenically similar and have overlapping histo-blood group antigen binding profiles[J]. mBio, 2018, 9(3):e00869-e00818. DOI:10. 1128/mbio. 00869-18.
[25]Wu Z, Yang L, Ren X, et al. Deciphering the bat virome catalog to better understand the ecological diversity of bat viruses and the bat origin of emerging infectious diseases[J]. ISME J, 2016, 10(3):609-620. DOI:10. 1038/ismej. 2015. 138.
[2] VinjéJ. Advances in laboratory methods for detection and typing of norovirus[J]. J Clin Microbiol, 2015, 53(2):373-381. DOI:10. 1128/jcm. 01535-14.
[3] Wang QH, Han MG, Cheetham S, et al. Porcine noroviruses related to human noroviruses[J]. Emerg Infect Dis, 2005, 11(12):1874-1881. DOI:10. 3201/eid1112. 050485.
[4] Chhabra P, de Graaf M, Parra GI, et al. Updated classification of norovirus genogroups and genotypes[J].J Gen Virol, 2019, 100(10):1393-1406. DOI:10. 1099/jgv. 0. 001318.
[5] de Graaf M, van Beek J, Koopmans MPG. Human norovirus transmission and evolution in a changing world[J]. Nat Rev Microbiol, 2016, 14:421-433. DOI:10. 1038/nrmicro. 2016. 48.
[6] Yoon HJ, Hur SH. Determination of safe contrast media dosage to estimated glomerular filtration rate ratios to avoid contrast-induced nephropathy after elective percutaneous coronary intervention[J]. Korean Circ J, 2011, 41(5):265-271. DOI:10. 4070/kcj. 2011. 41. 5. 265.
[7] Ramani S, Atmar RL, Estes MK. Epidemiology of human noroviruses and updates on vaccine development[J]. Curr Opin Gastroenterol, 2014, 30(1):25-33.DOI:10. 1097/mog. 0000000000000022.
[8] Patel MM, Widdowson MA, Glass RI, et al.Systematic literature review of role of noroviruses in sporadic gastroenteritis[J]. Emerg Infect Dis, 2008, 14(8):1224-1231. DOI:10. 3201/eid1408. 071114.
[9] Netzler NE, Enosi Tuipulotu D, White PA. Norovirus antivirals:Where are we now?[J]. Med Res Rev,2019, 39(3):860-886. DOI:10. 1002/med. 21545.
[10]Widdowson MA, Rockx B, Schepp R, et al. Detection of serum antibodies to bovine norovirus in veterinarians and the general population in the Netherlands[J]. J Med Virol, 2005, 76(1):119-128. DOI:10. 1002/jmv. 20333.
[11]Mesquita JR, Costantini VP, Cannon JL, et al.Presence of antibodies against genogroup VI norovirus in humans[J]. Virol J, 2013, 10:176. DOI:10. 1186/1743-422x-10-176.
[12]Humphrey TJ, Cruickshank JG, Cubitt WD. An outbreak of calicivirus associated gastroenteritis in an elderly persons home. A possible zoonosis?[J]. J Hyg(Lond), 1984, 93(2):293-299. DOI:10. 1017/s0022172400064822.
[13]Summa M, von Bonsdorff CH, Maunula L. Pet dogs—a transmission route for human noroviruses?[J]. J Clin Virol, 2012, 53(3):244-247. DOI:10. 1016/j.jcv. 2011. 12. 014.
[14]Caddy SL, de Rougemont A, Emmott E, et al.Evidence for human norovirus infection of dogs in the United Kingdom[J]. J Clin Microbiol, 2015, 53(6):1873-1883. DOI:10. 1128/jcm. 02778-14.
[15]Nakamura K, Saga Y, Iwai M, et al. Frequent detection of noroviruses and sapoviruses in swine and high genetic diversity of porcine sapovirus in Japan during Fiscal Year 2008[J]. J Clin Microbiol, 2010, 48(4):1215-1222. DOI:10. 1128/jcm. 02130-09.
[16]Bucardo F, González F, Reyes Y, et al.Seroprevalence in household raised pigs indicate high exposure to GII noroviruses in rural Nicaragua[J].Zoonoses Public Health, 2016, 63(8):600-607. DOI:10. 1111/zph. 12269.
[17]Liang Z, Zhang M, Wang Y, et al. Development and evaluation of a new luciferase immunosorbent assay to detect GII. 6 norovirus-specific IgG in different domestic and wild animals[J]. Front Microbiol, 2023, 14:1213007. DOI:10. 3389/fmicb. 2023. 1213007.
[18]Wang H, Cai Q, Liang Y, et al. A simple and highthroughput luciferase immunosorbent assay for both qualitative and semi-quantitative detection of anti-HIV-1antibodies[J]. Virus Res, 2019, 263:9-15. DOI:10. 1016/j. virusres. 2018. 12. 017.
[19]Bank-Wolf BR, K?nig M, Thiel HJ. Zoonotic aspects of infections with noroviruses and sapoviruses[J]. Vet Microbiol, 2010, 140(3-4):204-212. DOI:10. 1016/j. vetmic. 2009. 08. 021.
[20]王天禹，芜为，邓瑶，等.基于寨卡病毒NS2B蛋白特异抗原多肽的荧光素酶免疫吸附法检测寨卡病毒抗体[J].病毒学报，2019, 35(3):396-401. DOI:10. 13242/j. cnki. bingduxuebao. 003546.
[21]Hall MP, Unch J, Binkowski BF, et al. Engineered luciferase reporter from a deep sea shrimp utilizing a novel imidazopyrazinone substrate[J]. ACS Chem Biol,2012, 7(11):1848-1857. DOI:10. 1021/cb3002478.
[22]Baeshen MN, Al-Hejin AM, Bora RS, et al.Production of biopharmaceuticals in E. coli:current scenario and future perspectives[J]. J Microbiol Biotechnol, 2015, 25(7):953-962. DOI:10. 4014/jmb. 1412. 12079.
[23]Wolf S, Reetz J, Johne R, et al. The simultaneous occurrence of human norovirus and hepatitis E virus in a Norway rat(Rattus norvegicus)[J]. Arch Virol, 2013,158(7):1575-1578. DOI:10. 1007/s00705-013-1646-2.
[24]Kocher JF, Lindesmith LC, Debbink K, et al. Bat caliciviruses and human noroviruses are antigenically similar and have overlapping histo-blood group antigen binding profiles[J]. mBio, 2018, 9(3):e00869-e00818. DOI:10. 1128/mbio. 00869-18.
[25]Wu Z, Yang L, Ren X, et al. Deciphering the bat virome catalog to better understand the ecological diversity of bat viruses and the bat origin of emerging infectious diseases[J]. ISME J, 2016, 10(3):609-620. DOI:10. 1038/ismej. 2015. 138.
基本信息:
DOI：10.13242/j.cnki.bingduxuebao.004582
中图分类号:R512.5;R446.6
引用信息:
[1]王宇,张敏怡,徐嘉怡等.诺如病毒GⅡ.4 IgG抗体的荧光素酶免疫吸附方法的建立及在动物血清中的应用[J].病毒学报,2024,40(05):978-985.DOI:10.13242/j.cnki.bingduxuebao.004582.
基金信息:
广东省重点领域研发项目（项目号：2022B1111020002）,题目：华南地区主要流行人类病毒模式小鼠资源库的建立及评估应用;; 国家自然科学基金（项目号：31771007）,题目：GII.4型诺如病毒全人广谱中和单抗及其保守表位研究;; 广东省自然科学基金（项目号：2019A1515010951）,题目：基于自然人群队列诺如病毒基因型更迭的群体免疫机制研究~~
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