| 2,335 | 41 | 288 |
| 下载次数 | 被引频次 | 阅读次数 |
冠状病毒家族成员众多,主要感染哺乳动物和禽鸟类,给人类和动物的健康带来了极大危害。病毒对细胞表面受体的结合和内吞方式,决定病毒的宿主范围、组织嗜性和发病机理;此外,研究病毒的入胞途径不仅有助于我们认识病毒的生活周期,还对病毒性感染的预防和治疗具有重要意义。本文总结归纳了近十年来冠状病毒入胞途径的研究进展:多数冠状病毒可通过网格蛋白依赖型内吞、小窝蛋白依赖型内吞、巨胞饮以及网格蛋白/小窝蛋白非依赖型内吞途径进入细胞,到达早期内体、晚期内体或溶酶体等处发生膜融合,释放病毒基因组进入细胞质。病毒的内吞途径可作为广谱抗病毒药物设计的靶点。
Abstract:Coronavirus infects a number of mammalian and avian,causing great harm to the health of human and animal. Specific binding of the virus to the cell surface receptors and entry pathways determines the host range,tissue tropism and pathogenicity. In this article,we review and summarize the receptors and entry pathways employed by coronavirus. Most coronaviruses enter cells via clathrin-dependent endocytosis, caveolindependent endocytosis,pinocytosis,or clathrin/caveolin-independent endocytosis,reach early endosome,late endosome,or lysosome,finally fuse with membrane and release viral genome to cytoplasm. These entry pathways are the common target of anti-viral design.
[1]Drexler J F,Gloza-Rausch F,Glende J,Corman V M,Muth D,Goettsche M,Seebens A,Niedrig M,Pfefferle S,Yordanov S,Zhelyazkov L,Hermanns U,Vallo P,Lukashev A,Muller M A,Deng H,Herrler G,Drosten C.Genomic characterization of severe acute respiratory syndrome-related coronavirus in European bats and classification of coronaviruses based on partial RNA-dependent RNA polymerase gene sequences[J].JVirol,2010,84(21):11336-11349.
[2]Masters P S.The molecular biology of coronaviruses[J].Adv Virus Res,2006,66:193-292.
[3]Weiss S R,Leibowitz J L.Coronavirus pathogenesis[J].Adv Virus Res,2011,81:85-164.
[4]Gralinski L E,Baric R S.Molecular pathology of emerging coronavirus infections[J].J Pathol,2015,235(2):185-195.
[5]Chu V C,McElroy L J,Aronson J M,Oura T J,Harbison C E,Bauman B E,Whittaker G R.Feline aminopeptidase N is not a functional receptor for avian infectious bronchitis virus[J].Virol J,2007,4:20.
[6]边葶苈,周继勇,廖敏.冠状病毒非结构蛋白的研究进展[J].中国动物传染病学报,2013(4):67-74.
[7]Zhang J.Porcine deltacoronavirus:Overview of infection dynamics,diagnostic methods,prevalence and genetic evolution[J].Virus Res,2016,226:71-84.
[8]Heald-Sargent T,Gallagher T.Ready,set,fuse!The coronavirus spike protein and acquisition of fusion competence[J].Viruses,2012,4(4):557-580.
[9]Delmas B,Gelfi J,L'Haridon R,Vogel L K,Sjostrom H,Noren O,Laude H.Aminopeptidase N is a major receptor for the entero-pathogenic coronavirus TGEV[J].Nature,1992,357(6377):417-420.
[10]Yeager C L,Ashmun R A,Williams R K,Cardellichio C B,Shapiro L H,Look A T,Holmes K V.Human aminopeptidase N is a receptor for human coronavirus229E[J].Nature,1992,357(6377):420-422.
[11]Hohdatsu T,Izumiya Y,Yokoyama Y,Kida K,Koyama H.Differences in virus receptor for type I and type II feline infectious peritonitis virus[J].Arch Virol,1998,143(5):839-850.
[12]Benbacer L,Kut E,Besnardeau L,Laude H,Delmas B.Interspecies aminopeptidase-N chimeras reveal species-specific receptor recognition by canine coronavirus,feline infectious peritonitis virus,and transmissible gastroenteritis virus[J].J Virol,1997,71(1):734-737.
[13]Blau D M,Holmes K V.Human coronavirus HCoV-229E enters susceptible cells via the endocytic pathway[J].Adv Exp Med Biol,2001,494:193-198.
[14]Smith M K,Tusell S,Travanty E A,Berkhout B,van der Hoek L,Holmes K V.Human angiotensinconverting enzyme 2(ACE2)is a receptor for human respiratory coronavirus NL63[J].Adv Exp Med Biol,2006,581:285-288.
[15]Dimitrov D S.The secret life of ACE2 as a receptor for the SARS virus[J].Cell,2003,115(6):652-653.
[16]Wang H,Yang P,Liu K,Guo F,Zhang Y,Zhang G,Jiang C.SARS coronavirus entry into host cells through a novel clathrin-and caveolae-independent endocytic pathway[J].Cell Res,2008,18(2):290-301.
[17]Raj V S,Mou H,Smits S L,Dekkers D H,Muller MA,Dijkman R,Muth D,Demmers J A,Zaki A,Fouchier R A,Thiel V,Drosten C,Rottier P J,Osterhaus A D,Bosch B J,Haagmans B L.Dipeptidyl peptidase 4 is a functional receptor for the emerging human coronavirus-EMC[J].Nature,2013,495(7440):251-254.
[18]Huang X,Dong W,Milewska A,Golda A,Qi Y,Zhu Q K,Marasco W A,Baric R S,Sims A C,Pyrc K,Li W,Sui J.Human coronavirus HKU1 spike protein uses O-acetylated sialic acid as an attachment receptor determinant and employs hemagglutinin-esterase protein as a receptor-destroying enzyme[J].J Virol,2015,89(14):7202-7213.
[19]Adnan N,Nordin S M,Rahman I,Amini M H.Amarket modeling review study on predicting Malaysian consumer behavior towards widespread adoption of PHEV/EV[J].Environ Sci Pollut Res Int,2017,24(22):17955-17975.
[20]Dveksler G S,Pensiero M N,Cardellichio C B,Williams R K,Jiang G S,Holmes K V,Dieffenbach CW.Cloning of the mouse hepatitis virus(MHV)receptor:expression in human and hamster cell lines confers susceptibility to MHV[J].J Virol,1991,65(12):6881-6891.
[21]Madu I G,Chu V C,Lee H,Regan A D,Bauman BE,Whittaker G R.Heparan sulfate is a selective attachment factor for the avian coronavirus infectious bronchitis virus beaudette[J].Avian Diseases,2007,51(1):45-51.
[22]Bickerton E,Maier H J,Stevenson-Leggett P,Armesto M,Britton P.The S2 subunit of infectious bronchitis virus beaudette is a determinant of cellular tropism[J/OL].J Virol,2018,92(19).pii:e01044-18.
[23]Leung H S,Li O T,Chan R W,Chan M C,Nicholls JM,Poon L L.Entry of influenza A Virus with a alpha2,6-linked sialic acid binding preference requires host fibronectin[J].J Virol,2012,86(19):10704-10713.
[24]Li Q,Wei D,Feng F,Wang X L,Li C,Chen Z N,Bian H.alpha2,6-linked sialic acid serves as a highaffinity receptor for cancer oncolytic virotherapy with Newcastle disease virus[J].J Cancer Res Clin Oncol,2017,143(11):2171-2181.
[25]Winter C,Schwegmann-Wessels C,Cavanagh D,Neumann U,Herrler G.Sialic acid is a receptor determinant for infection of cells by avian Infectious bronchitis virus[J].J Gen Virol,2006,87(Pt 5):1209-1216.
[26]Winter C,Herrler G,Neumann U.Infection of the tracheal epithelium by infectious bronchitis virus is sialic acid dependent[J].Microbes Infect,2008,10(4):367-373.
[27]Wang B,Liu Y,Ji C M,Yang Y L,Liang Q Z,Zhao P,Xu L D,Lei X M,Luo W T,Qin P,Zhou J,Huang Y W.Porcine deltacoronavirus engages the transmissible gastroenteritis virus functional receptor porcine aminopeptidase N for infectious cellular entry[J/OL].J Virol,2018,92(12).pii:e00318-18.
[28]Danthi P,Tosteson M,Li Q H,Chow M.Genome delivery and ion channel properties are altered in VP4mutants of poliovirus[J].J Virol,2003,77(9):5266-5274.
[29]Borsa J,Morash B D,Sargent M D,Copps T P,Lievaart P A,Szekely J G.Two modes of entry of reovirus particles into L cells[J].J Gen Virol,1979,45(1):161-170.
[30]Harding C,Heuser J,Stahl P.Endocytosis and intracellular processing of transferrin and colloidal goldtransferrin in rat reticulocytes:demonstration of a pathway for receptor shedding[J].Eur J Cell Biol,1984,35(2):256-263.
[31]Keen J H.Clathrin and associated assembly and disassembly proteins[J].Annu Rev Biochem,1990,59:415-438.
[32]Stenmark H,Parton R G,Steele-Mortimer O,Lutcke A,Gruenberg J,Zerial M.Inhibition of rab5 GTPase activity stimulates membrane fusion in endocytosis[J].EMBO J,1994,13(6):1287-1296.
[33]Stone M,Jia S,Heo W D,Meyer T,Konan K V.Participation of rab5,an early endosome protein,in hepatitis C virus RNA replication machinery[J].J Virol,2007,81(9):4551-4563.
[34]Hernaez B,Guerra M,Salas M L,Andres G.African swine fever virus undergoes outer envelope disruption,capsid disassembly and inner envelope fusion before core release from multivesicular endosomes[J/OL].PLoSPathog,2016,12(4):e1005595.
[35]Damm E M,Pelkmans L,Kartenbeck J,Mezzacasa A,Kurzchalia T,Helenius A.Clathrin-and caveolin-1-independent endocytosis:entry of simian virus 40 into cells devoid of caveolae[J].J Cell Biol,2005,168(3):477-488.
[36]Marjomaki V,Pietiainen V,Matilainen H,Upla P,Ivaska J,Nissinen L,Reunanen H,Huttunen P,Hyypia T,Heino J.Internalization of echovirus 1 in caveolae[J].J Virol,2002,76(4):1856-1865.
[37]Henley J R,Krueger E W,Oswald B J,McNiven M A.Dynamin-mediated internalization of caveolae[J].J Cell Biol,1998,141(1):85-99.
[38]Vainio S,Heino S,Mansson J E,Fredman P,Kuismanen E,Vaarala O,Ikonen E.Dynamic association of human insulin receptor with lipid rafts in cells lacking caveolae[J].EMBO Rep,2002,3(1):95-100.
[39]Rawat S S,Viard M,Gallo S A,Rein A,Blumenthal R,Puri A.Modulation of entry of enveloped viruses by cholesterol and sphingolipids(Review)[J].Mol Membr Biol,2003,20(3):243-254.
[40]Tan L,Zhang Y,Qiao C,Yuan Y,Sun Y,Qiu X,Meng C,Song C,Liao Y,Munir M,Nair V,Ding Z,Liu X,Ding C.NDV entry into dendritic cells through macropinocytosis and suppression of T lymphocyte proliferation[J].Virology,2018,518:126-135.
[41]Saeed M F,Kolokoltsov A A,Albrecht T,Davey R A.Cellular entry of ebola virus involves uptake by a macropinocytosis-like mechanism and subsequent trafficking through early and late endosomes[J/OL].PLoS Pathog,2010,6(9):e1001110.
[42]Marechal V,Prevost M C,Petit C,Perret E,Heard JM,Schwartz O.Human immunodeficiency virus type 1entry into macrophages mediated by macropinocytosis[J].J Virol,2001,75(22):11166-11177.
[43]Pelkmans L,Puntener D,Helenius A.Local actin polymerization and dynamin recruitment in SV40-induced internalization of caveolae[J].Science,2002,296(5567):535-539.
[44]Sabharanjak S,Sharma P,Parton R G,Mayor S.GPI-anchored proteins are delivered to recycling endosomes via a distinct cdc42-regulated,clathrin-independent pinocytic pathway[J].Dev Cell,2002,2(4):411-423.
[45]Chadda R,Howes M T,Plowman S J,Hancock J F,Parton R G,Mayor S.Cholesterol-sensitive Cdc42activation regulates actin polymerization for endocytosis via the GEEC pathway[J].Traffic,2007,8(6):702-717.
[46]Li F,Berardi M,Li W,Farzan M,Dormitzer P R,Harrison S C.Conformational states of the severe acute respiratory syndrome coronavirus spike protein ectodomain[J].J Virol,2006,80(14):6794-6800.
[47]Zelus B D,Schickli J H,Blau D M,Weiss S R,Holmes K V.Conformational changes in the spike glycoprotein of murine coronavirus are induced at 37degrees C either by soluble murine CEACAM1receptors or by pH 8[J].J Virol,2003,77(2):830-840.
[48]Gallagher T M,Buchmeier M J.Coronavirus spike proteins in viral entry and pathogenesis[J].Virology,2001,279(2):371-374.
[49]Yamada Y,Liu D X.Proteolytic activation of the spike protein at a novel RRRR/S motif is implicated in furindependent entry,syncytium formation,and infectivity of coronavirus infectious bronchitis virus in cultured cells[J].J Virol,2009,83(17):8744-8758.
[50]Belouzard S,Chu V C,Whittaker G R.Activation of the SARS coronavirus spike protein via sequential proteolytic cleavage at two distinct sites[J].Proc Natl Acad Sci U S A,2009,106(14):5871-5876.
[51]Milewska A,Nowak P,Owczarek K,Szczepanski A,Zarebski M,Hoang A,Berniak K,Wojarski J,Zeglen S,Baster Z,Rajfur Z,Pyrc K.Entry of Human Coronavirus NL63 into the Cell[J/OL].J Virol,2018,92(3).pii:e01933-17.
[52]Nomura R,Kiyota A,Suzaki E,Kataoka K,Ohe Y,Miyamoto K,Senda T,Fujimoto T.Human coronavirus 229E binds to CD13 in rafts and enters the cell through caveolae[J].J Virol,2004,78(16):8701-8708.
[53]Park J E,Cruz D J,Shin H J.Clathrin-and serine proteases-dependent uptake of porcine epidemic diarrhea virus into Vero cells[J].Virus Res,2014,191:21-29.
[54]Van Hamme E,Dewerchin H L,Cornelissen E,Verhasselt B,Nauwynck H J.Clathrin-and caveolaeindependent entry of feline infectious peritonitis virus in monocytes depends on dynamin[J].J Gen Virol,2008,89(Pt 9):2147-2156.
[55]Hilgenfeld R,Peiris M.From SARS to MERS:10years of research on highly pathogenic human coronaviruses[J].Antiviral Res,2013,100(1):286-295.
[56]Millet J K,Whittaker G R.Host cell entry of Middle East respiratory syndrome coronavirus after two-step,furin-mediated activation of the spike protein[J].Proc Natl Acad Sci U S A,2014,111(42):15214-15219.
[57]Pu Y,Zhang X.Mouse hepatitis virus type 2 enters cells through a clathrin-mediated endocytic pathway independent of Eps15[J].J Virol,2008,82(16):8112-8123.
[58]Li Z,Zhao K,Lan Y,Lv X,Hu S,Guan J,Lu H,Zhang J,Shi J,Yang Y,Song D,Gao F,He W.Porcine Hemagglutinating Encephalomyelitis Virus Enters Neuro-2a Cells via Clathrin-Mediated Endocytosis in a Rab5-,Cholesterol-,and pH-Dependent Manner[J/OL].J Virol,2017,91(23).pii:e01083-17.
[59]Chu V C,McElroy L J,Chu V,Bauman B E,Whittaker G R.The avian coronavirus infectious bronchitis virus undergoes direct low-pH-dependent fusion activation during entry into host cells[J].J Virol,2006,80(7):3180-3188.
[60]Wang H,Yuan X,Sun Y,Mao X,Meng C,Tan L,Song C,Qiu X,Ding C,Liao Y.Infectious bronchitis virus entry mainly depends on clathrin mediated endocytosis and requires classical endosomal/lysosomal system[J].Virology,2018,528:118-136.
基本信息:
DOI:10.13242/j.cnki.bingduxuebao.003586
中图分类号:S852.65
引用信息:
[1]王欢,丁铲,廖瑛.冠状病毒入胞途径的研究进展[J].病毒学报,2019,35(06):964-971.DOI:10.13242/j.cnki.bingduxuebao.003586.
基金信息:
国家自然科学基金(项目号:31772724),题目:应激颗粒作为抗病毒天然免疫信号枢纽的研究; 中国农业科学院上海兽医研究所中央级公益性科研院所基本科研业务费专项资金项目(项目号:2017JB05),题目:传染性支气管炎病毒的入胞方式和脱壳位点的研究~~
2019-09-12
2019-09-12
2019-09-12