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EV71-B5亚型自2003年首次报道以来,在亚太地区频繁引发手足口病(Hand,foot and mouth disease,HFMD)疫情,并可引起部分婴幼儿重症表现甚至死亡。为了阐明EV71-B5亚型在亚太地区的流行病学规律及进化特征,本文采用分子生物学软件(主要包括BioEdit、MEGA 5.0、Jmodeltest2.1.7、BEAST1.8.2、Tracer1.5、FigTree1.4.3、SPREAD1.0.7),对60条亚太地区的EV71-B5亚型序列进行进化分析及系统发育地理学分析。分析结果显示EV71-B5亚型可在同一地区(中国台湾、泰国等)频繁流行,最近共同祖先的起源时间为2000年9月(95%CI:1999年8月~2001年9月),平均进化速度为4.045×10-3(95%CI:3.222×10-34.909×10-3);此外,迁移图谱表明EV71-B5亚型在亚太地区具有明显的迁移传播现象,中国厦门EV71-B5亚型分离株(JN964686)推测来源于新加坡地区(BF=3.17)。这种现象提示我国需要加强对该亚型的监测力度,以免发生大范围暴发。
Abstract：Since its first identification,the EV71-B5 subgenogroup has been showed to triggere hand,foot and mouth diseases in Asian-Pacific Region,and to cause severe manifestations among some infants(even death).We wished to understand the epidemiology,transmission,pathogenic origin and evolution of the EV71-B5 subgenogroup. Molecular biology-based softwares(BioEdit,MEGA3.0,Jmodeltest2.1.7,BEAST1.8.2,Tracer1.5,FigTree1.4.3,SPREAD1.0.7)were used to make phylogenetic trees and undertake phylogeographic analyses on 60 sequences of the EV71-B5 subgenotype.Results showed that EV71-B5 subgenotype could spread within the same district,and that its most recent common ancestorwas inSeptember,2000(95% confidence interval(CI):August,1999 to September,2001).Median speed of evolution is 4.045×10-3(95%CI:3.2224.909×10-3).The EV71-B5 subgenotype has an obvious rule of migration transmission,and we speculate that isolation of the EV71-B5 subgenotype in southern China is derived from Singapore(Bayes Factor=3.17).In conclusions,the EV71-B5 subgenotype showsactive migration among the Asian-Pacific region,and our epidemic surveillance should be strengthened.
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基本信息:
DOI：10.13242/j.cnki.bingduxuebao.003148
中图分类号:R725.1
引用信息:
[1]吴文辉,陈梦媛,张师音,等.EV71-B5亚型在亚太地区流行病学及进化分析[J].病毒学报,2017,33(03):404-412.DOI:10.13242/j.cnki.bingduxuebao.003148.
基金信息:
福建省自然科学基金(项目号:2013J06016),题目:厦门地区手足口病病原谱监测及基因进化趋势研究~~
2017-04-19
2017-04-19
2017-04-19