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为研究辽宁省水痘-带状疱疹病毒（Varicella zoster virus,VZV）gK、gH、gM、gL四种糖蛋白基因特征，应用聚合酶链（Polymerase chain reaction,PCR）方法对辽宁省2017-2020年31株VZV gK、gH、gM、gL四种糖蛋白进行全基因扩增，并对扩增产物进行序列测定，分析不同位点核苷酸及氨基酸的变异情况。辽宁省31株VZV与v-Oka疫苗株（AB097932）相比，LN-VZV gK、gH、gM、gL共有16个位点发生核苷酸突变，导致氨基酸发生同义突变12处，错义突变4处。导致氨基酸发生错义突变包括：1株在4490位点发生A→G的碱基突变，导致编码氨基酸由I→T;1株在4730位点发生C→T的碱基突变，导致编码氨基酸由R→K;3株在67528位点发生A→G的碱基突变，导致编码氨基酸由I→M;1株在101579位点发生A→C的碱基突变，导致编码氨基酸由L→R。与Dumas株（X04370）相比，31株LN-VZV和v-Oka疫苗株在gL的101623-101624位点发生ATC的碱基插入，导致VZV gL内插入一个甲硫氨酸。31株LN-VZV与GenBank中各基因型VZV代表株相比核苷酸和氨基酸的同源性均大于98.10%。辽宁省VZV gK、gH、gM、gL基因序列高度保守，抗原性稳定，本研究首次对辽宁地区VZV糖蛋白序列进行测序分析，为辽宁省VZV的监测、疫苗效果评估及VZV进一步研究提供实验室依据。
Abstract：This study aimed to investigate the characteristics of the gK, gH, gM, and gL glycoprotein genes of Varicella Zoster Virus(VZV) in Liaoning Province, China. Whole gene amplification of the gK, gH, gM, and gL genes of 31 VZV strains collected from Liaoning Province, China between 2017 and 2020 was performed using Polymerase Chain Reaction(PCR). The amplified products were sequenced, and nucleotide and amino acid variations at different sites were analyzed.Compared with the v-Oka vaccine strain, 16 nucleotide mutations were detected in the gK, gH, gM, and gL genes, resulting in 12 synonymous mutations and 4 missense mutations in amino acids. The missense mutations included: an A→G base mutation at position 4490 in one strain, leading to an amino acid change from I to T; a C→T base mutation at position 4730 in one strain, resulting in an amino acid change from R to K; an A→G base mutation at position 67528 in three strains, causing an amino acid change from I to M; and an A→C base mutation at position 101579 in one strain, leading to an amino acid change from L to R. Compared with Dumas strain, both the 31 LN-VZV strains and the v-Oka vaccine strain exhibited an ATC base insertion at positions 101623–101624 in gL, resulting in the insertion of a methionine in the VZV gL protein. The nucleotide and amino acid homology of the 31 LN-VZV strains exceeded 98.10% compared with representative VZV genotypes in GenBank. The sequences of the gK, gH, gM, and gL genes of VZV in Liaoning Province, China were found to be highly conserved and antigenically stable. This study represents the first analysis of VZV glycoprotein sequences in Liaoning Province and provides a laboratory foundation for VZV monitoring, vaccine efficacy evaluation, and further research on VZV in Liaoning Province, China.
[1] Prazsák I, Moldován N, Balázs Z, et al. Long-read sequencing uncovers a complex transcriptome topology in Varicella zoster virus[J]. BMC Genomics, 2018, 19(1):873. DOI:10. 1186/s12864-018-5267-8.
[2]吴秋华，许松涛，齐梦缘，等.中国六省市12例水痘-带状疱疹患者病毒糖蛋白E基因的序列特征分析[J].病毒学报，2018, 34(6):877-883. DOI:10. 13242/j.cnki. bingduxuebao. 003469.
[3] Breuer J. Molecular genetic insights into Varicella zoster virus(VZV), the vOka vaccine strain, and the pathogenesis of latency and reactivation[J]. J Infect Dis, 2018, 218(suppl_2):S75-S80. DOI:10. 1093/infdis/jiy279.
[4]牟君杰，齐梦缘，李崇山，等.中国六省份水痘-带状疱疹病毒糖蛋白M、L基因特征分析[J].中华预防医学杂志，2018, 52(4):419-423. DOI:10. 3760/cma. j.issn. 0253-9624. 2018. 04. 016.
[5] Rider PJF, Coghill LM, Naderi M, et al. Identification and visualization of functionally important domains and residues in herpes simplex virus glycoprotein K(gK)using a combination of phylogenetics and protein modeling[J]. Sci Rep, 2019, 9(1):14625. DOI:10. 1038/s41598-019-50490-9.
[6] Storlie J, Maresova L, Jackson W, et al. Comparative analyses of the 9 glycoprotein genes found in wild-type and vaccine strains of Varicella-zoster virus[J]. J Infect Dis, 2008, 197(Suppl 2):S49-S53. DOI:10. 1086/522127.
[7] Mo C, Suen J, Sommer M, et al. Characterization of Varicella-Zoster virus glycoprotein K(open reading frame 5)and its role in virus growth[J]. J Virol, 1999,73(5):4197-4207. DOI:10. 1128/JVI. 73. 5. 4197-4207. 1999.
[8] Yang E, Arvin AM, Oliver SL. The cytoplasmic domain of Varicella-zoster virus glycoprotein H regulates syncytia formation and skin pathogenesis[J].PLoS Pathog, 2014, 10(5):e1004173. DOI:10. 1371/journal. ppat. 1004173.
[9] Hall SL, Govero JL, Heineman TC. Intracellular transport and stability of Varicella-zoster virus glycoprotein K[J]. Virology, 2007, 358(2):283-290.DOI:10. 1016/j. virol. 2006. 08. 021.
[10]Cohen JI. The Varicella-zoster virus genome[J]. Curr Top Microbiol Immunol, 2010, 342:1-14. DOI:10. 1007/82_2010_10.
[11]Oliver SL, Zhou M, Arvin AM. Varicella-zoster virus:molecular controls of cell fusion-dependent pathogenesis[J]. Biochem Soc Trans, 2020, 48(6):2415-2435. DOI:10. 1042/BST20190511.
[12]Bae S, Song YJ. Inhibition of Varicella-zoster virus replication by an ethanol extract of Lysimachia mauritiana[J]. Mol Med Rep, 2017, 15(6):3847-3851. DOI:10. 3892/mmr. 2017. 6444.
[13]KutinováL, Hainz P, LudvíkováV, et al. Immune response to vaccinia virus recombinants expressing glycoproteins GE, gB, gH, and gL of Varicella-zoster virus[J]. Virology, 2001, 280(2):211-220. DOI:10. 1006/viro. 2000. 0754.
[14]Yamagishi Y, Sadaoka T, Yoshii H, et al. Varicellazoster virus glycoprotein M homolog is glycosylated, is expressed on the viral envelope, and functions in virus cell-to-cell spread[J]. J Virol, 2008, 82(2):795-804.DOI:10. 1128/JVI. 01722-07.
[15]Zerboni L, Sung P, Sommer M, et al. The C-terminus of Varicella-zoster virus glycoprotein M contains trafficking motifs that mediate skin virulence in the SCID-human model of VZV pathogenesis[J]. Virology,2018, 523:110-120. DOI:10. 1016/j.virol. 2018. 08. 003.
[16]付文锟，王玮，刘剑，等.水痘-带状疱疹病毒糖蛋白N的单克隆抗体制备及初步研究[J].病毒学报，2017,33(3):380-388. DOI:10. 13242/j. cnki.bingduxuebao. 003160.
[17]Sadaoka T, Yanagi T, Yamanishi K, et al.Characterization of the Varicella-zoster virus ORF50gene, which encodes glycoprotein M[J]. J Virol, 2010,84(7):3488-3502. DOI:10. 1128/JVI. 01838-09.
[18]齐梦缘，吴秋华，杨玉颖，等.中国六省水痘-带状疱疹病毒糖蛋白基因特征分析[J].病毒学报，2017, 33(2):156-162. DOI:10. 13242/j. cnki.bingduxuebao. 003115.
[19]Oliver SL, Yang E, Arvin AM. Varicella-zoster virus glycoproteins:entry, replication, and pathogenesis[J].Curr Clin Microbiol Rep, 2016, 3(4):204-215. DOI:10. 1007/s40588-016-0044-4.
[20]Wagenaar TR, Chow VT, Buranathai C, et al. The out of Africa model of Varicella-zoster virus evolution:single nucleotide polymorphisms and private alleles distinguish Asian clades from European/north American clades[J]. Vaccine, 2003, 21(11-12):1072-1081.DOI:10. 1016/s0264-410x(02)00559-5.
[21]Jiang L, Gan L, Chen J, et al. Genetic analysis of clinical VZV isolates collected in China reveals a more homologous profile[J]. Biomed Res Int, 2013, 2013:681234. DOI:10. 1155/2013/681234.
[22]Faga B, Maury W, Bruckner DA, et al. Identification and mapping of single nucleotide polymorphisms in the Varicella-zoster virus genome[J]. Virology, 2001, 280(1):1-6. DOI:10. 1006/viro. 2000. 0775.
[23]Jeon JS, Won YH, Kim IK, et al. Analysis of single nucleotide polymorphism among Varicella-Zoster Virus and identification of vaccine-specific sites[J]. Virology,2016, 496:277-286. DOI:10. 1016/j.virol. 2016. 06. 017.
[24]Sauerbrei A, Zell R, Harder M, et al. Genotyping of different Varicella vaccine strains[J]. J Clin Virol,2006, 37(2):109-117. DOI:10. 1016/j.jcv. 2006. 07. 002.
[25]Bakker KM, Eisenberg MC, Woods RJ, et al.Identifying optimal vaccination scenarios to reduce Varicella zoster virus transmission and reactivation[J].BMC Med, 2022, 20(1):387. DOI:10. 1186/s12916-022-02534-7.
[26]Sorel O,Messaoudi I. Insights into the pathogenesis of varicella viruses[J]. Curr Clin Microbiol Rep,2019,6(3):156-165. DOI:10. 1007/s40588-019-00119-2.
[27]Wang M, Li X, You M, et al. Epidemiological characteristics of Varicella outbreaks-China, 2006-2022[J]. China CDC Wkly, 2023, 5(52):1161-1166.DOI:10. 46234/ccdcw2023. 218.
基本信息:
DOI：10.13242/j.cnki.bingduxuebao.004663
中图分类号:R373
引用信息:
[1]王文思,王樱槥,方兴,等.辽宁省水痘-带状疱疹病毒K、H、M、L糖蛋白基因特征分析[J].病毒学报,2025,41(01):99-106.DOI:10.13242/j.cnki.bingduxuebao.004663.
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