2024 06 v.40 1397-1403
EBV感染患儿异型淋巴细胞和PBL比值与EB病毒载量的关系研究
基金项目(Foundation):
邮箱(Email):
ljf0809l@163.com;
DOI:
10.13242/j.cnki.bingduxuebao.004608
中文作者单位:
广州市红十字会医院(暨南大学附属广州红十字会医院);
摘要(Abstract):
为探究Epstein-Barr病毒（Epstein-Barr,EBV）感染患儿异型淋巴细胞比值和外周血淋巴细胞（Peripheral blood lymphocyte,PBL）比值与EBV-DNA载量的关系，选取2022年10月～2023年12月广州市红十字会医院收治的186例EBV感染患儿作为此次研究对象，运用RT-qPCR技术检测其外周血EBV-DNA载量，并根据EBV-DNA载量将患儿分为EBV-DNA阳性组和EBV-DNA阴性组，检测患者血常规，统计分析淋巴细胞数据，并通过显微镜镜检外周血涂片，计数异型淋巴细胞，最终计算其在淋巴细胞中的百分比。应用Pearson相关性分析EBV感染患儿异型淋巴细胞比值和PBL比值与EBV-DNA载量的相关性。结果发现，共有73例患儿EBV-DNA呈阳性，阳性率为39.25%，且EBV-DNA阳性组载量显著高于阴性组（P<0.05）；不同性别间EBV-DNA阳性率无显著差异（P>0.05），但1～3岁患儿阳性率显著高于其他年龄段（P<0.05）；进一步分析发现，EBV-DNA阳性组患儿的异型淋巴细胞比值和PBL比值均显著高于阴性组（P<0.05）;Pearson相关性分析揭示，随着EBV-DNA载量增加，异型淋巴细胞比值和PBL比值均呈上升趋势，且两者与EBV-DNA载量均呈正相关。本研究表明，EBV-DNA载量与异型淋巴细胞及PBL比值密切相关，且1～3岁患儿EBV-DNA阳性率显著较高。因此，监测上述指标对于EBV感染患儿的临床诊断、病情监控及制定年龄特异性治疗策略具有重要意义。
关键词(KeyWords):
EB病毒;异型淋巴细胞;外周血淋巴细胞;EB病毒载量;相关性
| 114 | 0 | 103 |
| 下载次数 | 被引频次 | 阅读次数 |
参考文献
[1] Damania B, Kenney SC, Raab-Traub N. Epstein-Barr virus:Biology and clinical disease[J]. Cell, 2022, 185(20):3652-3670. DOI:10. 1016/j. cell. 2022. 08. 026.
[2] Soldan SS, Lieberman PM. Epstein–Barr virus and multiple sclerosis[J]. Nat Rev Microbiol, 2023, 21:51-64. DOI:10. 1038/s41579-022-00770-5.
[3] Bjornevik K, Münz C, Cohen JI, et al. Epstein–Barr virus as a leading cause of multiple sclerosis:mechanisms and implications[J]. Nat Rev Neurol,2023, 19:160-171. DOI:10. 1038/s41582-023-00775-5.
[4]王莉娟，李丹，王芳，等. EBV感染及STING/TBK1信号通路与儿童传染性单核细胞增多症临床表现的关系[J].中华医院感染学杂志，2022, 32(21):3331-3335.
[5]唐旭东，胡灵灵，沈子园，等. EBV相关噬血细胞综合征临床特征和预后分析[J].南京医科大学学报（自然科学版），2022, 42(12):1697-1702, 1709. DOI:10. 7655/NYDXBNS20221209.
[6] Nagata K, Hayashi K, Kumata K, et al. Epstein-Barr virus reactivation in peripheral B lymphocytes induces IgM-type thyrotropin receptor autoantibody production in patients with Graves'disease[J]. Endocr J, 2023, 70(6):619-627. DOI:10. 1507/endocrj. ej22-0609.
[7]段红梅，申昆玲.慢性活动性EB病毒感染的诊断标准和治疗[J].实用儿科临床杂志，2006, 21(10):638-640. DOI:10. 3969/j. issn. 1003-515X. 2006. 10. 037.
[8] Lin J, Chen X, Wu H, et al. Peripheral blood lymphocyte counts in patients with infectious mononucleosis or chronic active Epstein-Barr virus infection and prognostic risk factors of chronic active Epstein-Barr virus infection[J]. Am J Transl Res,2021, 13(11):12797-12806.
[9]吕晓丽，张喆，王希，等.西安地区临床患者EBV感染血清学特征及疾病谱分析[J].现代检验医学杂志，2023, 38(2):136-139, 154. DOI:10. 3969/j.issn. 1671-7414. 2023. 02. 025.
[10]陈建琴，吴建刚，王朝晖，等. EB病毒伴肺炎支原体感染致IM的临床特征及发病的危险因素分析[J].中国病原生物学杂志，2022, 17(3):344-347. DOI:10. 13350/j. cjpb. 220321.
[11]El-Mallawany NK, Curry CV, Allen CE.Haemophagocytic lymphohistiocytosis and Epstein-Barr virus:a complex relationship with diverse origins,expression and outcomes[J]. Br J Haematol, 2022, 196(1):31-44. DOI:10. 1111/bjh. 17638.
[12]Alami IE, Gihbid A, Charoute H, et al. Prognostic value of Epstein-Barr virus DNA load in nasopharyngeal carcinoma:a meta-analysis[J]. Pan Afr Med J, 2022,41:6. DOI:10. 11604/pamj. 2022. 41. 6. 28946.
[13]Yi X, Wen CH, Gu SQ, et al. Hepatitis B virus antigen peptide presentation by Epstein-Barr virustransformed peripheral blood B cells[J]. Zhonghua Gan Zang Bing Za Zhi, 2021, 29(3):240-245. DOI:10. 3760/cma. j. cn501113-20190717-00253.
[14]张叶萌，张莹，张延霞.慢性活动性EBV感染患者外周血淋巴细胞表达水平及预后危险因素分析[J].西部医学，2023, 35(11):1699-1705, 1710. DOI:10. 3969/j. issn. 1672-3511. 2023. 11. 026.
[15]Wei A, Ou W, Zhao Y, et al. Clinical characteristics of peripheral lymphocyte subtypes in chronic active EpsteinBarr virus infection[J]. J Infect Dis, 2024, 230(1):95-102. DOI:10. 1093/infdis/jiad435.
[16]Xu M, Li Y, Cao M, et al. Expression and clinical significance of peripheral blood IL-17A, IL-22, tim-3,and gal-9 in children with infectious mononucleosis[J].Viral Immunol, 2023, 36(7):458-465. DOI:10. 1089/vim. 2022. 0203.
[17]马永梅，樊荣.传染性单核细胞增多症患儿血清Toll样受体2、Toll样受体9与外周血T淋巴细胞亚群变化及临床意义[J].陕西医学杂志，2022, 51(11):1416-1419. DOI:10. 3969/j. issn. 1000-7377. 2022. 11. 023.
[18]Qian J, Yu Q, Chen G, et al. Altered ratio of circulating follicular regulatory T cells and follicular helper T cells during primary EBV infection[J]. Clin Exp Med, 2020, 20(3):373-380. DOI:10. 1007/s10238-020-00621-8.
[19]Zhang R, Mao Z, Xu C, et al. Association between antibiotic exposure and the risk of rash in children with infectious mononucleosis:a multicenter, retrospective cohort study[J]. Antimicrob Agents Chemother, 2023,67(6):e0024923. DOI:10. 1128/aac. 00249-23.
[20]Ishikawa Y, Yamada M, Wada N, et al. Mucosalassociated invariant T cells are activated in an interleukin-18-dependent manner in Epstein-Barr virus-associated T/natural killer cell lymphoproliferative diseases[J]. Clin Exp Immunol, 2022, 207(2):141-148. DOI:10. 1093/cei/uxab004.
[2] Soldan SS, Lieberman PM. Epstein–Barr virus and multiple sclerosis[J]. Nat Rev Microbiol, 2023, 21:51-64. DOI:10. 1038/s41579-022-00770-5.
[3] Bjornevik K, Münz C, Cohen JI, et al. Epstein–Barr virus as a leading cause of multiple sclerosis:mechanisms and implications[J]. Nat Rev Neurol,2023, 19:160-171. DOI:10. 1038/s41582-023-00775-5.
[4]王莉娟，李丹，王芳，等. EBV感染及STING/TBK1信号通路与儿童传染性单核细胞增多症临床表现的关系[J].中华医院感染学杂志，2022, 32(21):3331-3335.
[5]唐旭东，胡灵灵，沈子园，等. EBV相关噬血细胞综合征临床特征和预后分析[J].南京医科大学学报（自然科学版），2022, 42(12):1697-1702, 1709. DOI:10. 7655/NYDXBNS20221209.
[6] Nagata K, Hayashi K, Kumata K, et al. Epstein-Barr virus reactivation in peripheral B lymphocytes induces IgM-type thyrotropin receptor autoantibody production in patients with Graves'disease[J]. Endocr J, 2023, 70(6):619-627. DOI:10. 1507/endocrj. ej22-0609.
[7]段红梅，申昆玲.慢性活动性EB病毒感染的诊断标准和治疗[J].实用儿科临床杂志，2006, 21(10):638-640. DOI:10. 3969/j. issn. 1003-515X. 2006. 10. 037.
[8] Lin J, Chen X, Wu H, et al. Peripheral blood lymphocyte counts in patients with infectious mononucleosis or chronic active Epstein-Barr virus infection and prognostic risk factors of chronic active Epstein-Barr virus infection[J]. Am J Transl Res,2021, 13(11):12797-12806.
[9]吕晓丽，张喆，王希，等.西安地区临床患者EBV感染血清学特征及疾病谱分析[J].现代检验医学杂志，2023, 38(2):136-139, 154. DOI:10. 3969/j.issn. 1671-7414. 2023. 02. 025.
[10]陈建琴，吴建刚，王朝晖，等. EB病毒伴肺炎支原体感染致IM的临床特征及发病的危险因素分析[J].中国病原生物学杂志，2022, 17(3):344-347. DOI:10. 13350/j. cjpb. 220321.
[11]El-Mallawany NK, Curry CV, Allen CE.Haemophagocytic lymphohistiocytosis and Epstein-Barr virus:a complex relationship with diverse origins,expression and outcomes[J]. Br J Haematol, 2022, 196(1):31-44. DOI:10. 1111/bjh. 17638.
[12]Alami IE, Gihbid A, Charoute H, et al. Prognostic value of Epstein-Barr virus DNA load in nasopharyngeal carcinoma:a meta-analysis[J]. Pan Afr Med J, 2022,41:6. DOI:10. 11604/pamj. 2022. 41. 6. 28946.
[13]Yi X, Wen CH, Gu SQ, et al. Hepatitis B virus antigen peptide presentation by Epstein-Barr virustransformed peripheral blood B cells[J]. Zhonghua Gan Zang Bing Za Zhi, 2021, 29(3):240-245. DOI:10. 3760/cma. j. cn501113-20190717-00253.
[14]张叶萌，张莹，张延霞.慢性活动性EBV感染患者外周血淋巴细胞表达水平及预后危险因素分析[J].西部医学，2023, 35(11):1699-1705, 1710. DOI:10. 3969/j. issn. 1672-3511. 2023. 11. 026.
[15]Wei A, Ou W, Zhao Y, et al. Clinical characteristics of peripheral lymphocyte subtypes in chronic active EpsteinBarr virus infection[J]. J Infect Dis, 2024, 230(1):95-102. DOI:10. 1093/infdis/jiad435.
[16]Xu M, Li Y, Cao M, et al. Expression and clinical significance of peripheral blood IL-17A, IL-22, tim-3,and gal-9 in children with infectious mononucleosis[J].Viral Immunol, 2023, 36(7):458-465. DOI:10. 1089/vim. 2022. 0203.
[17]马永梅，樊荣.传染性单核细胞增多症患儿血清Toll样受体2、Toll样受体9与外周血T淋巴细胞亚群变化及临床意义[J].陕西医学杂志，2022, 51(11):1416-1419. DOI:10. 3969/j. issn. 1000-7377. 2022. 11. 023.
[18]Qian J, Yu Q, Chen G, et al. Altered ratio of circulating follicular regulatory T cells and follicular helper T cells during primary EBV infection[J]. Clin Exp Med, 2020, 20(3):373-380. DOI:10. 1007/s10238-020-00621-8.
[19]Zhang R, Mao Z, Xu C, et al. Association between antibiotic exposure and the risk of rash in children with infectious mononucleosis:a multicenter, retrospective cohort study[J]. Antimicrob Agents Chemother, 2023,67(6):e0024923. DOI:10. 1128/aac. 00249-23.
[20]Ishikawa Y, Yamada M, Wada N, et al. Mucosalassociated invariant T cells are activated in an interleukin-18-dependent manner in Epstein-Barr virus-associated T/natural killer cell lymphoproliferative diseases[J]. Clin Exp Immunol, 2022, 207(2):141-148. DOI:10. 1093/cei/uxab004.
基本信息:
DOI：10.13242/j.cnki.bingduxuebao.004608
中图分类号:R725.1
引用信息:
[1]刘剑烽,吴伟,张锐.EBV感染患儿异型淋巴细胞和PBL比值与EB病毒载量的关系研究[J].病毒学报,2024,40(06):1397-1403.DOI:10.13242/j.cnki.bingduxuebao.004608.
基金信息: