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从患肌肉白浊症状的罗氏沼虾幼苗体内分离纯化得到两种大小不同的病毒颗粒。这两种病毒颗粒均为对称的 2 0面体结构 ,表面光滑 ,无囊膜 ,对氯仿不敏感。一种是直径为 2 6nm～ 2 7nm的颗粒 ,在氯化铯中的密度为1 32 g/cm3 ,病毒基因组含两段单链的RNA ,分别为 3 0kb和 1 2kb ,具有诺达病毒科成员的特征。一种是直径为1 4nm～ 1 6nm的颗粒 ,在氯化铯中的密度为 1 33g/cm3 ,含有一段大小为 0 9kb的单链RNA ,拟为卫星病毒样颗粒或辅助病毒
Abstract：Two kinds of viral particles were isolated and purified from freshwater prawn Macrobrachium rosenbergii Both of them possess icosahedral,non enveloped and smooth surface structure,and are not sensitive to chloroform The larger one is 26mm-27nm in diameter and has a mean buyant density of 1 32g/cm 3 in CsCl Its genome contains two ssRNA segments with the size of 3 0kb and 1 2kb,respectively This viral particle is close to the family Nodaviridae The smaller particle is 14nm-16nm in diameter and has a mean buyant density of 1 33g/cm 3 in CsCl Its genome contains one 0 9kb ssRNA segment This viral particle was supposed to be satellite like virus or helper like virus
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基本信息:
中图分类号:S945.41
引用信息:
[1]石正丽,钱冬,张建红,曹铮,Bonami Jean Robert.罗氏沼虾体内两种病毒颗粒的分离、纯化与核酸特性[J].病毒学报,2004(01):58-61.
基金信息:
中国科学院与法国科研中心科技合作协议项目 ;; 中科院武汉病毒所无脊椎动物病毒学联合开放实验室开放课题
2004-03-30
2004-03-30