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为分离并鉴定中国大陆输入性B3基因型麻疹病毒。使用淋巴信号激活因子转染的非洲绿猴肾细胞(Vero/Slam细胞)对该麻疹病例的咽拭子标本进行病毒分离,经逆转录聚合酶链反应(Reverse transcript polymerase chain reaction,RT-PCR)方法扩增出核蛋白(Nucleoprotein,N)羧基(COOH)端核苷酸片段,并进行序列测定和分析。结果表明这两株B3基因型麻疹病毒分离株和世界卫生组织(World Health Organization,WHO)B3基因型参考株MVi/Ibadan.NGA/97在基因亲缘性关系树上同属一个分支,核苷酸同源性均为98%;与麻疹核苷酸序列数据管理系统(Measles nucleotide surveillance,MeaNS)中的病毒序列进行比对,结果表明本研究分离的两株麻疹病毒与2013年同期在澳大利亚、日本、韩国,中国香港地区,菲律宾、伊朗等国家流行的B3基因型麻疹病毒亲缘性关系为100%。在中国上海分离到的麻疹毒株为B3基因型。是中国自1993年开展病毒学监测以来首次分离到B3基因型病毒,为国外输入性的麻疹病毒。本研究中分离到的B3基因型麻疹病毒对于积累中国麻疹分子流行病学基线数据具有很重要的意义,也有助于全国及全球麻疹病毒的传播及溯源。
Abstract：We isolated and identified the genotypes and molecular characteristics of the imported B3 measles virus(MeV)in the Chinese mainland.The Vero/SLAM cell line was used to isolate the viruses.Reverse transcription-polymerase chain reaction was undertaken to amplify the 450 nucleotide acids of the 3-terminal of the nucleoprotein gene.A phylogenetic tree was constructed and similarities in homology assessed.Results suggested that the Shanghai isolates MVi/Shanghai.CHN/38.13/02[B3]and MVi/Shanghai.CHN/40.13/02[B3]were clustered within the same genotype group as the World Health Organization(WHO)B3genotype reference strain.The number of differences in nucleotide acids between the two Shanghai isolates was one.The homology of nucleotide acids between the Shanghai isolates and the WHO B3 genotype reference strain(MVi/Ibadan.NGA/0.97/1/B3)was 98%.Comparative results from the Measles Nucleotide Surveillance system suggested that the sequences of Shanghai isolates and the 2013 viruses from Australia,Japan,Korea,Hong Kong China,Philippines and Iran were identical.This is the first time that the B3 genotype of MeV in the Chinese mainland has been isolated since 1993.These data can be used to create a "baseline" of genetic information for measles viruses in China,and help to trace the transmission of measles viruses in China and the rest of the world.
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基本信息:
DOI：10.13242/j.cnki.bingduxuebao.002544
中图分类号:R373.9
引用信息:
[1]王淑蕾,李崇山,王慧玲,等.中国大陆首次发现输入性B3基因型麻疹病毒病例[J].病毒学报,2014,30(05):535-540.DOI:10.13242/j.cnki.bingduxuebao.002544.
基金信息:
国家自然科学基金课题(项目号:81371791);; 国家科学技术重大专项艾滋病和病毒性肝炎等重大传染病防治课题(编号:2012ZX10004215、2012ZX10004401、2013ZX10004202和2013ZX10004101);重大新药创制课题(编号:2013ZX09304101);; 世界卫生组织西太平洋区麻疹参比实验室的职能(编号:WP CHN AAA 011XZ 08)
2014-09-23
2014-09-23
2014-09-23