nav emailalert searchbtn searchbox tablepage yinyongbenwen piczone journalimg qikanlogo popupnotification paper
2024 03 v.40 447-457
衔接蛋白复合物1γ1亚基影响SARS-CoV-2感染性的分析
基金项目(Foundation): 国家重点研发计划项目(项目号:2018YFE0107600),题目:靶向活化胞内固有免疫在病毒性传染病防控中的应用~~
邮箱(Email): wentaoqiao@nankai.edu.cn;
DOI: 10.13242/j.cnki.bingduxuebao.004517
中文作者单位:

南开大学生命科学学院;

摘要(Abstract):

新型冠状病毒(Severe acute respiratory syndrome coronavirus 2, SARS-CoV-2)引发了全球大流行的新型冠状病毒感染(Coronavirus disease 2019, COVID-19),对人类健康和社会经济发展造成极大威胁,深入研究其感染复制机制对防控该病毒致病及流行至关重要。有研究发现SARS-CoV-2可通过网格蛋白介导的内吞、转运系统进入细胞建立感染。衔接蛋白复合物1 γ1亚基(Adaptor related protein complex 1 subunit gamma 1, AP1G1)是衔接蛋白复合物1(Adapter protein 1, AP-1)的重要组成部分,参与胞吞、囊泡转运等生理过程。本文基于相关文献报道选择AP1G1展开研究,确证其对SARS-CoV-2感染性的影响并初探机制。首先借鉴文献,建立了SARS-CoV-2病毒样颗粒(SARS-CoV-2 virus like particles, SC2-VLPs)系统作为报告病毒,发现过表达AP1G1降低以携带萤火虫荧光素酶为报告基因的SC2-VLPs-Luc的感染性;敲减内源AP1G1增强SC2-VLPs-Luc的感染性;发现AP1G1可被包装入SC2-VLPs-Luc,虽未改变SC2-VLPs-Luc中病毒结构蛋白组成,但影响SC2-VLPs-Luc递送mRNA能力。本研究为解析AP1G1功能及其对SARS-CoV-2感染性的影响提供了新的实验证据。

关键词(KeyWords): SC2-VLPs;;AP1G1;;SARS-CoV-2
参考文献 [1] Giovanetti M, Benedetti F, Campisi G, et al.Evolution patterns of SARS-CoV-2:Snapshot on its genome variants[J]. Biochem Biophys Res Commun,2021, 538:88-91. DOI:10. 1016/j.bbrc. 2020. 10. 102.
[2] Mercer J, Helenius A. Virus entry by macropinocytosis[J]. Nat Cell Biol, 2009, 11(5):510-520. DOI:10. 1038/ncb0509-510.
[3] Zhou H, Costaguta G, Payne G S. Clathrin adaptor complex-interacting protein irc6 functions through the conserved c-terminal domain[J]. Sci Rep, 2019, 9(1):4436-4448. DOI:10. 1038/s41598-019-40852-8.
[4] Meier O, Boucke K, Hammer S V, et al. Adenovirus triggers macropinocytosis and endosomal leakage together with its clathrin-mediated uptake[J]. J Cell Biol, 2002, 158(6):1119-1131. DOI:10. 1083/jcb. 200112067.
[5] Shi R, Hou L, Wei L, et al. Involvement of adaptor proteins in clathrin-mediated endocytosis of virus entry[J]. Microb Pathog, 2021, 161(Pt A):105278. DOI:10. 1016/j. micpath. 2021. 105278.
[6] Boehm M, Bonifacino J S. Adaptins:the final recount[J]. Mol Biol Cell, 2001, 12(10):2907-2920. DOI:10. 1091/mbc. 12. 10. 2907.
[7] Traub L M. Tickets to ride:selecting cargo for clathrinregulated internalization[J]. Nat Rev Mol Cell Biol,2009, 10(9):583-596. DOI:10. 1038/nrm2751.
[8] Braulke T, Bonifacino J S. Sorting of lysosomal proteins[J]. Biochim Biophys Acta, 2009, 1793(4):605-614.DOI:10. 1016/j. bbamcr. 2008. 10. 016.
[9] Ohno H, Stewart J, Fournier M C, et al. Interaction of tyrosine-based sorting signals with clathrin-associated proteins[J]. Science, 1995, 269(5232):1872-1875.DOI:10. 1126/science. 7569928.
[10]Stephens D J, Crump C M, Clarke A R, et al. Serine331 and tyrosine 333 are both involved in the interaction between the cytosolic domain of TGN38 and the mu2subunit of the AP2 clathrin adaptor complex[J]. J Biol Chem, 1997, 272(22):14104-14109. DOI:10. 1074/jbc. 272. 22. 14104.
[11]Chaudhuri R, Lindwasser O W, Smith W J, et al.Downregulation of CD4 by human immunodeficiency virus type 1 Nef is dependent on clathrin and involves direct interaction of Nef with the AP2 clathrin adaptor[J]. J Virol, 2007, 81(8):3877-3890. DOI:10. 1128/JVI. 02725-06.
[12]Doray B, Lee I, Knisely J, et al. The gamma/sigma1and alpha/sigma2 hemicomplexes of clathrin adaptors AP-1 and AP-2 harbor the dileucine recognition site[J].Mol Biol Cell, 2007, 18(5):1887-1896. DOI:10. 1091/mbc. e07-01-0012.
[13]Ohno H. Clathrin-associated adaptor protein complexes[J]. J Cell Sci, 2006, 119(Pt 18):3719-3721. DOI:10. 1242/jcs. 03085.
[14]Candiello E, Kratzke M, Wenzel D, et al. AP-1/sigma1A and AP-1/sigma1B adaptor-proteins differentially regulate neuronal early endosome maturation via the Rab5/Vps34-pathway[J]. Sci Rep,2016, 6:29950. DOI:10. 1038/srep29950.
[15]Kratzke M, Candiello E, Schmidt B, et al. AP-1/sigma1B-Dependent SV Protein Recycling Is Regulated in Early Endosomes and Is Coupled to AP-2 Endocytosis[J]. Mol Neurobiol, 2015, 52(1):142-161. DOI:10. 1007/s12035-014-8852-0.
[16]Bayati A, Kumar R, Francis V, et al. SARS-CoV-2infects cells after viral entry via clathrin-mediated endocytosis[J]. J Biol Chem, 2021, 296:100306.DOI:10. 1016/j. jbc. 2021. 100306.
[17]Israeli M, Finkel Y, Yahalom-Ronen Y, et al. Genome-wide CRISPR screens identify GATA6 as a proviral host factor for SARS-CoV-2 via modulation of ACE2[J]. Nat Commun, 2022, 13(1):2237-2252. DOI:10. 1038/s41467-022-29896-z.
[18]Rebendenne A, Roy P, Bonaventure B, et al.Bidirectional genome-wide CRISPR screens reveal host factors regulating SARS-CoV-2, MERS-CoV and seasonal HCoVs[J]. Nat Genet, 2022, 54(8):1090-1102. DOI:10. 1038/s41588-022-01110-2.
[19]Ozono S, Zhang Y, Ode H, et al. SARS-CoV-2D614G spike mutation increases entry efficiency with enhanced ACE2-binding affinity[J]. Nat Commun,2021, 12(1):848-856. DOI:10. 1038/s41467-021-21118-2
[20]Essalmani R, Jain J, Susan-Resiga D, et al. Distinctive Roles of Furin and TMPRSS2 in SARS-CoV-2Infectivity[J]. J Virol, 2022, 96(8):e0012822. DOI:10. 1128/jvi. 00128-22.
[21]Syed A M, Taha T Y, Tabata T, et al. Rapid assessment of SARS-CoV-2-evolved variants using virus-like particles[J]. Science, 2021, 374(6575):1626-1632. DOI:10. 1126/science. abl6184.
[22]Hetrick B, Chilin L D, He S, et al. Development of a hybrid alphavirus-SARS-CoV-2 pseudovirion for rapid quantification of neutralization antibodies and antiviral drugs[J]. Cell Rep Methods, 2022, 2(3):100181.DOI:10. 1016/j. crmeth. 2022. 100181.
[23]Zizioli D, Meyer C, Guhde G, et al. Early embryonic death of mice deficient in gamma-adaptin[J]. J Biol Chem, 1999, 274(9):5385-5390. DOI:10. 1074/jbc. 274. 9. 5385.
[24]Meyer C, Zizioli D, Lausmann S, et al. mu1A-adaptindeficient mice:Lethality, loss of AP-1 binding and rerouting of mannose 6-phosphate receptors[J]. EMBO J, 2000, 19(10):2193-2203. DOI:10. 1093/emboj/19. 10. 2193.
[25]Hase K, Nakatsu F, Ohmae M, et al. AP-1B-mediated protein sorting regulates polarity and proliferation of intestinal epithelial cells in mice[J]. Gastroenterology,2013, 145(3):625-635. DOI:10. 1053/j.gastro. 2013. 05. 013.
[26]Tao X, Lu Y, Qiu S, et al. AP1G1 is involved in cetuximab-mediated downregulation of ASCT2-EGFR complex and sensitization of human head and neck squamous cell carcinoma cells to ROS-induced apoptosis[J]. Cancer Lett, 2017, 408:33-42. DOI:10. 1016/j.canlet. 2017. 08. 012.
[27]Tavares L A, da Silva E M, da Silva-Januário M E, et al. CD4 downregulation by the HIV-1 protein Nef reveals distinct roles for the gamma1 and gamma2subunits of the AP-1 complex in protein trafficking[J]. J Cell Sci, 2017, 130(2):429-443. DOI:10. 1242/jcs. 192104.
[28]Chen D, Zheng Q, Sun L, et al. ORF3a of SARS-CoV-2 promotes lysosomal exocytosis-mediated viral egress[J]. Dev Cell, 2021, 56(23):3250-3263. DOI:10. 1016/j. devcel. 2021. 10. 006.

基本信息:

DOI:10.13242/j.cnki.bingduxuebao.004517

中图分类号:R373

引用信息:

[1]纪月,邱曼曼,谈娟等.衔接蛋白复合物1γ1亚基影响SARS-CoV-2感染性的分析[J].病毒学报,2024,40(03):447-457.DOI:10.13242/j.cnki.bingduxuebao.004517.

基金信息:

国家重点研发计划项目(项目号:2018YFE0107600),题目:靶向活化胞内固有免疫在病毒性传染病防控中的应用~~

检 索 高级检索

引用

GB/T 7714-2015 格式引文
MLA格式引文
APA格式引文