南开大学生命科学学院;
新型冠状病毒（Severe acute respiratory syndrome coronavirus 2, SARS-CoV-2）引发了全球大流行的新型冠状病毒感染（Coronavirus disease 2019, COVID-19），对人类健康和社会经济发展造成极大威胁，深入研究其感染复制机制对防控该病毒致病及流行至关重要。有研究发现SARS-CoV-2可通过网格蛋白介导的内吞、转运系统进入细胞建立感染。衔接蛋白复合物1 γ1亚基（Adaptor related protein complex 1 subunit gamma 1, AP1G1）是衔接蛋白复合物1(Adapter protein 1, AP-1)的重要组成部分，参与胞吞、囊泡转运等生理过程。本文基于相关文献报道选择AP1G1展开研究，确证其对SARS-CoV-2感染性的影响并初探机制。首先借鉴文献，建立了SARS-CoV-2病毒样颗粒（SARS-CoV-2 virus like particles, SC2-VLPs）系统作为报告病毒，发现过表达AP1G1降低以携带萤火虫荧光素酶为报告基因的SC2-VLPs-Luc的感染性；敲减内源AP1G1增强SC2-VLPs-Luc的感染性；发现AP1G1可被包装入SC2-VLPs-Luc，虽未改变SC2-VLPs-Luc中病毒结构蛋白组成，但影响SC2-VLPs-Luc递送mRNA能力。本研究为解析AP1G1功能及其对SARS-CoV-2感染性的影响提供了新的实验证据。
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基本信息:
DOI：10.13242/j.cnki.bingduxuebao.004517
中图分类号:R373
引用信息:
[1]纪月,邱曼曼,谈娟等.衔接蛋白复合物1γ1亚基影响SARS-CoV-2感染性的分析[J].病毒学报,2024,40(03):447-457.DOI:10.13242/j.cnki.bingduxuebao.004517.
基金信息:
国家重点研发计划项目（项目号：2018YFE0107600）,题目：靶向活化胞内固有免疫在病毒性传染病防控中的应用~~