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为分析新疆2023-2024年乙型Victoria系流感病毒血凝素基因特征，本文从新疆15家国家级流感监测网络实验室分离上送的BV系流感毒株中随机选取14株，进行二代全基因组序列测定。通过生物信息学软件MEGA X、BioEdit、ChiPot、NetNGlyc 1.0 Services进行基因序列特征及同源性分析。2023-2024年度共监测流感毒株14份，抗原性分析显示，所有毒株均为B/Austria/1359417/2021(BV)疫苗株的类似株，未检测到低反应株；与疫苗株相比，核苷酸同源性为98.8%～99.5%，氨基酸同源性为99.4%～99.8%;14株BV系流感毒株HA基因与疫苗亲缘关系极高，均以162-164位三缺失为主要分支特征，共同归属于V1A.3a.2分支；共2个位点发生氨基酸变异，分别为E128D(11/14)和E183K(11/14)，仅E128D位点处于120环，属于关键位点变异；所有流感毒株均未发现糖基化位点的置换变异。2023-2024年新疆BV系流感毒株与疫苗株进化起源相同，未发现新的变异株，提示该组分疫苗匹配性好，但仍发现潜在的非关键位点的变异，故今后仍需加强流感监测工作，为BV系流感病毒的分子进化规律以及流行病学监测提供研究基础。
Abstract：To analyze the hemagglutinin(HA) gene characteristics of Victoria lineage type B influenza virus circulating in Xinjiang, China from 2023 to 2024, 14 BV influenza strains were randomly selected from isolates submitted by 15 national influenza surveillance network laboratories. Whole-genome sequencing was performed using next-generation sequencing technology. Bioinformatics tools, including MEGA X, BioEdit, ChiPot, and NetNGlyc 1.0 Services, were used for gene sequence characterization and homology analysis. A total of 14influenza strains were analyzed in the 2023–2024 season. Antigenicity analysis showed that all strains were similar to the vaccine strain B/Austria/1359417/2021(BV), with no low-reactive strains detected. Nucleotide homology with the vaccine strain ranged from 98.8% to 99.5%, while amino acid homology ranged from 99.4%to 99.8%. Phylogenetic analysis revealed that the HA genes of all 14 BV influenza strains were closely related to the vaccine strain, with a characteristic three-amino-acid deletion at positions 162 – 164, classifying them within the V1A.3a2 subclade. Two amino acid substitutions were identified: E128D(11/14) and E183K(11/14), with E128D located within the 120-loop, a critical antigenic site. No glycosylation site substitutions were observed among the analyzed strains. The 2023– 2024 BV influenza strains in Xinjiang, China share the same evolutionary origin as the vaccine strain, with no novel variant strains detected, indicating good vaccine strain match. However, the presence of potential non-critical site mutations underscores the need for continued influenza surveillance to support the study of BV influenza virus molecular evolution and epidemiological monitoring.
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基本信息:
DOI：10.13242/j.cnki.bingduxuebao.240291
中图分类号:R373.13
引用信息:
[1]陈媛,张璇,黄佳,等.2023-2024年新疆乙型Victoria系流感病毒血凝素基因特征分析[J].病毒学报,2025,41(04):1097-1104.DOI:10.13242/j.cnki.bingduxuebao.240291.
基金信息:
新疆维吾尔自治区重点研发计划项目（项目号：2021B03003-1）,题目：新型冠状病毒肺炎防控关键技术研究; 2025年自治区卫生健康委科技项目（项目号：2025001QNKYXM650024279）,题目：季节性H3N2流感病毒高产低变株鸡胚模型的建立~~
2025-06-12
2025-06-12
2025-06-12