| 530 | 117 | 20 |
| 下载次数 | 被引频次 | 阅读次数 |
通过接种DF-1细胞(C/E)系,从山东某地方品系芦花鸡的鸡群中分离到一株外源性白血病病毒(ALV)SDAU09C2。与GenBank中已发表的不同亚群鸡ALV参考株的囊膜蛋白gp85的氨基酸序列比较,表明该分离株与B亚群ALV(ALV-B)2个参考株的gp85的氨基酸同源性最高,均为92.5%;与A、C、D、E亚群ALV的gp85的氨基酸同源性仅在73.2%87.9%之间;而与J亚群gp85的氨基酸同源性更低至30.3%32.4%。这是我国地方品系鸡群中第一次分离和鉴定ALV-B及其gp85基因的报道。
Abstract：By inoculation of blood samples in DF-1(C/E) cell cultlure,an exogenous avian leukosis virus(ALV) strain SDAU09C2 was isolated from a breeder farm of Chinese native breed "Luhua" in Shandong province.Comparisons of the amino acid sequence of env gene gp85 from the isolate with those from other ALV reference strains of different subgroups indicated that SDAU09C2 had the highest gp85 identity to two reference strains of subgroup B of 92.5%.Its gp85 identity to other chicken ALV subgroups A,C,D,E was in the range of 73.2%-87.9%.The identity to subgroup J was only 30.3%-32.4%.This is the first report on isolation and identification of ALV-B and its gp85 from Chinese native breed chickens.
[1]Fadly A M,Nair V.Leukosis/Sarcoma Group[M].//Saif Y M,Fadly A M,Glison J R,et al.Disease ofpoultry.ed.12th.Blackwell Publishing Ames,2008:514-568.
[2]Payne L N,Brown S R,Bumstead K,et al.A novelsubgroup of exogenous avian leukosis virus in chickens[J].J Gen Virol,1991,72:801-807.
[3]Payne L N,Howes K,Gillespie A M,et al.Host rangeof Rous sarcoma virus pseudotype RSV(HPRS-103)in12 avian species:support for a new avian retrovirus en-velope subgroup,designated J[J].J Gen Virol,1992,73:2995-2997.
[4]Fadly A M,Smith E J.Isolation and some characteristicsof a subgroup J-like avian leukosis virus associated withmyeloid leukosis in meat-type chickens in the UnitedStates[J].Avian Dis,1999,43:391-400.
[5]杜岩,崔治中,秦爱建,等.鸡的J亚群白血病病毒的分离及部分序列比较[J].病毒学报,2000,16:341-346.
[6]Cui Z,Du Y,Zhang Z,et al.Comparison of Chinesefield strains of avian leukosis subgroup J viruses withprototype strain HPRS-103 and United States strains[J].Avian Dis,2003,47:1321-1330.
[7]张志,崔治中,赵宏坤,等.商品代肉鸡J亚群禽白血病的病理及病毒分离鉴定[J].中国兽医杂志,2002,38:6-8.
[8]Xu,B,Dong,W,Yu C,et al.Occurrence of avian leu-kosis virus subgroup J in commercial layer flocks in Chi-na[J].Avian Pathol,2004,33:13-17.
[9]王辉,崔治中.蛋鸡J亚群白血病病毒的分离鉴定与序列分析[J].病毒学报,2008,24:369-375.
[10]Sun S H,Cui Z Z.Epidemiological and pathologicalstudies of subgroup J avian leukosis virus infectious inchinese local“yellow”chicken[J].Avian Pathol,2007,36:221-226.
[11]成子强,张利,刘思当,等.中国麻鸡中发现禽J亚群白血病[J].微生物学报,2005,45:584-587
[12]Silva R F,Fadly A M,Hunt H D.Hypervariability inthe envelope genes of subgroup J avian leukosis virusesobtained from different farms in the United States[J].Virology,2000,272:106-111.
[13]Silva R F,Fadly A M,Taylor S P.Development of apolymerase chain reaction to differentiate avain leukosisvirus(ALV)subgroup:detection of an ALV contami-nant in commercial Marek's disease vaccines[J].AvainDis,2007,51:663-667.
[14]Zavala G,Cheng S.Detection and characterization of a-vian leukosis virus in Marek's disease vaccines[J].Avi-an Dis,2006,50:209-215.
[15]Bova C A,Manfredi J P,Ronald S.Env genes of avianretroviruses:nucleotide sequence and molecular recom-binants define host range determinants[J].Virology,1986,152:343-354.
[16]Bieth E,Darlix J L.Complete nucleotide sequence of ahighly infectious avian leukosis virus[J].NucleicAcids Res,1992,20:367.
[17]Bizub D,Katz R A,Skalka A M.Nucleotide sequenceof noncoding regions in Rous-associated virus-2:com-parisons delineate conserved regions important in repli-cation and oncogenesis[J].J Virol,1984,49:557-565.
[18]Dorner A J,Stoye J P,Coffin J M.Molecular basis ofhost range variation in avian retroviruses[J].J Virol,1985,53:32-39.
[19]Barbosa T,Zavala G,Cheng S.Molecualr characteriza-tion of three recombinant isolates of avian leukosis virusobtained from contaminated Marek's disease vaccines[J].Avian Dis,2008,52:245-252.
基本信息:
DOI：10.13242/j.cnki.bingduxuebao.002053
中图分类号:S852.65
引用信息:
[1]赵冬敏,张青婵,崔治中.芦花鸡中B亚群禽白血病病毒的分离与鉴定[J].病毒学报,2010,26(01):53-57.DOI:10.13242/j.cnki.bingduxuebao.002053.
基金信息:
山东省2008~2009年农业重大应用技术创新课题;; 国家农业公益性行业科研专项经费项目(200803019)资助~~