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2024 03 v.40 484-491
痘苗病毒天坛株N1L基因表达调控序列的克隆与特性分析
基金项目(Foundation):
邮箱(Email): wangwl@ivdc.chinacdc.cn;
DOI: 10.13242/j.cnki.bingduxuebao.004519
中文作者单位:

中国疾病预防控制中心病毒病预防控制所传染病溯源预警和智能决策全国重点实验室;

摘要(Abstract):

本研究以痘苗病毒天坛株(Vaccinia Virus Tian Tan Strain,VTT)为对象,旨在明确N1L基因的表达调控序列。我们克隆了N1L起始密码子ATG上游不同长度的调控序列片段,构建了表达载体,并利用报告基因萤火虫荧光素酶(Firefly luciferase,Fluc)的表达来确定N1L表达调控序列区域。通过PROMO在线预测网站,预测了与调控序列结合的转录因子。结果显示,VTT中N1L基因的表达受到上游调控序列的调控,其中以-404bp起始的片段启动的Fluc表达量最高。PROMO预测表明该区域存在多个转录因子结合位点。本研究为痘苗病毒基因表达特点的研究提供了实验依据,并为N1L基因功能及机制研究奠定了基础。

关键词(KeyWords): 痘苗病毒天坛株;;N1L基因;;基因表达与调控;;转录通读
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基本信息:

DOI:10.13242/j.cnki.bingduxuebao.004519

中图分类号:R373

引用信息:

[1]翟玉倩,韩一泽,吴长城等.痘苗病毒天坛株N1L基因表达调控序列的克隆与特性分析[J].病毒学报,2024,40(03):484-491.DOI:10.13242/j.cnki.bingduxuebao.004519.

基金信息:

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