| 269 | 20 | 21 |
| 下载次数 | 被引频次 | 阅读次数 |
大豆花叶病毒外壳蛋白基因克隆及在病毒检测中的应用周雪平，濮祖芹，方中达，储瑞银，陈章良（南京农业大学植物保护学系南京２１００１４）关键词大豆花叶病毒，外壳蛋白基因，分子杂交分布于世界各国大豆种植区的大豆花叶病毒（ＳＭＶ），能引起大豆花叶病，造成大豆减...
Abstract：sing SMV─Sa RNA as template, SMV cDNA was synthesized by AMV reverse tran-scriptase. The synthesized cDNA was then amplified by polymerase chain reaction (PC.ADNA fragment with 0.8kb was obtained after 30 PCR amplification. The restriction map of the DNA fragment was analyzed. The results show the coat protein gene of SMV Sa strain has been cloned. The BamHI restriction endonuclease fragments of pSMV61 was labeled by nick translation and used in dot-blot hybridization, as little as 0.4ng of purified virus were de-tected. Spot hybridization can also be applied to detect SMV in soybean leaves.
1BosL．SoybeanMosaicVirus．CMI／AABDescriptionsofPlantViruses，1972，No．932MauleAJ，etal．TheapplicationofspothybridizationtothedetectionofDNAandRNAvirusesinplanttissues．J3李尉民，濮祖芹．大豆花叶病毒（SMV）提纯方法的比较研究．南京农业大学学报，1990，13（4增）：56─594EggenbergerAL，etal．Thenucleotidesequenceofasoybeanmosaicviruscoatprotein－codingregionanditsexpres－sioninEscherichiacoli，Agrobacteriumtumefaciensandtobaccocallus．JGenVirol，1989，70：1853─18605ManiatisT，etal．Molecularcloning，ALaboratoryManual．Newyork，USA：ColdSpringHarbourLabPress，1982，365─3706GallitelliD，etal．Relationshipsamongvirusesinthetombusvirusgroup：nucleicacidhybridizationstudies．JGen7RoyBP，etal．ConstructionanduseofclonedcDNAbiotinand32P－labeledprobesforthedetectionofpapa－yamosaicprtexvirusRNAinplants．Phytopathology，1988，78：1425－14298BaulcombeD，etal．ThesensitivityandspecificityofarapidnucleixacidhybridizationmethodforthedetectionofpotatovirusXincrudesapsamples．PlantPathology，1984，33：361─370
基本信息:
中图分类号:S432.41
引用信息:
[1]周雪平，濮祖芹，方中达，储瑞银，陈章良.大豆花叶病毒外壳蛋白基因克隆及在病毒检测中的应用[J].病毒学报,1994(01):.
1994-03-30
1994-03-30