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2025 01 v.41 39-47
不同基因型麻疹病毒株细胞吸附力、复制力及致细胞病变能力的测定
基金项目(Foundation): 福建省科技创新平台建设项目(项目号:2019Y2001),题目:福建省预防医学研究院建设~~
邮箱(Email): xiuxiuyxh@126.com;
DOI: 10.13242/j.cnki.bingduxuebao.004676
中文作者单位:

福建医科大学公共卫生学院;福建省疾病预防控制中心福建省人兽共患病重点实验室;宁德市疾病预防控制中心;

摘要(Abstract):

麻疹病毒基因型流行数量在逐年下降,但B3和D8基因型病毒株却频频出现,并成为近年唯一发现在流行的两个野病毒基因型;我国本土H1基因型在2019年后未再检出。本研究从细胞水平分析了输入B3和D8基因型病毒株、本土H1基因型不同年代分离株及疫苗株在Vero/SLAM细胞上吸附力、复制力和致细胞病变能力的差异性。结果发现,不同分离株间存在不同程度表现型的差异,有可能影响其传播流行的强度。与S_(191)疫苗株相比,B3基因型代表株MV19-1144(B3)在Vero/SLAM细胞上虽具有较低的吸附能力,却有较强复制能力和致细胞病变能力,能快速地在较短时间内达到拷贝数和病毒量的高峰;另一代表株MV18-55(B3)细胞吸附力更强些,早期同样不存在下降期,就开始病毒较强的复制和增殖。MV19-1214(D8)具有较强的吸附能力,更容易感染宿主细胞,但是病毒复制力和致细胞病变能力则较弱;MV20-17(D8)却具有不一样的表现,其吸附力较MV19-1214(D8)弱些,但复制和致细胞病变能力更接近疫苗株病毒。福建省近期的H1基因型分离株MV15-278(H1a)和MV16-189(H1a)虽然在吸附力方面和早期H1分离株相比无统计学差别,但先后出现了复制力和致细胞病变能力的下降,提示在强大的免疫压力下,本土病毒株可能出现了突变,减缓了病毒传播和流行的速度,最终使得H1a基因型在福建的阻断。

关键词(KeyWords): 麻疹病毒;拷贝数;吸附力;复制力;致细胞病效应
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基本信息:

DOI:10.13242/j.cnki.bingduxuebao.004676

中图分类号:R373.11

引用信息:

[1]尤娜,张梦萍,张苏晗等.不同基因型麻疹病毒株细胞吸附力、复制力及致细胞病变能力的测定[J].病毒学报,2025,41(01):39-47.DOI:10.13242/j.cnki.bingduxuebao.004676.

基金信息:

福建省科技创新平台建设项目(项目号:2019Y2001),题目:福建省预防医学研究院建设~~

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