| 147 | 0 | 243 |
| 下载次数 | 被引频次 | 阅读次数 |
麻疹病毒基因型流行数量在逐年下降，但B3和D8基因型病毒株却频频出现，并成为近年唯一发现在流行的两个野病毒基因型；我国本土H1基因型在2019年后未再检出。本研究从细胞水平分析了输入B3和D8基因型病毒株、本土H1基因型不同年代分离株及疫苗株在Vero/SLAM细胞上吸附力、复制力和致细胞病变能力的差异性。结果发现，不同分离株间存在不同程度表现型的差异，有可能影响其传播流行的强度。与S191疫苗株相比，B3基因型代表株MV19-1144(B3)在Vero/SLAM细胞上虽具有较低的吸附能力，却有较强复制能力和致细胞病变能力，能快速地在较短时间内达到拷贝数和病毒量的高峰；另一代表株MV18-55(B3)细胞吸附力更强些，早期同样不存在下降期，就开始病毒较强的复制和增殖。MV19-1214(D8)具有较强的吸附能力，更容易感染宿主细胞，但是病毒复制力和致细胞病变能力则较弱；MV20-17(D8)却具有不一样的表现，其吸附力较MV19-1214(D8)弱些，但复制和致细胞病变能力更接近疫苗株病毒。福建省近期的H1基因型分离株MV15-278(H1a)和MV16-189(H1a)虽然在吸附力方面和早期H1分离株相比无统计学差别，但先后出现了复制力和致细胞病变能力的下降，提示在强大的免疫压力下，本土病毒株可能出现了突变，减缓了病毒传播和流行的速度，最终使得H1a基因型在福建的阻断。
Abstract：The number of circulating measles viruses(MeV) genotypes has been decreasing year by years, but B3 and D8 genotypes have frequently emerged and are now the only two circulating wild-type genotypes in recent years. The local Chinese H1 genotype has not been detected since 2019. In this study, the adsorption, replication, and cytopathic effect of imported B3 and D8 genotype strains, local H1 genotype strains from different years, and vaccine strains were analyzed on Vero/SLAM cells at the cellular level. The results revealed phenotypic differences among the strains, which may influence their epidemic potential. Compared to the vaccine virus S191, the B3 representative strain MV19-1144(B3) exhibited lower adsorption capacity on Vero/SLAM cells but demonstrated higher replication ability and a stronger cytopathic effect, rapidly reaching a peak in virus load and copy number in a short period. Another B3 strain, MV18-55(B3), showed stronger adsorption capacity and initiated replication and viral multiplication early, without an initial decline. MV19-1214(D8) exhibited stronger adsorption capacity and more readily infected host cells, but its replication ability and cytopathic effect were weaker. In contrast, MV20-17(D8) showed a different phenotype, with weaker adsorption capacity compared to MV19-1214(D8), but its replication and cytopathic effect were more similar to those of the vaccine strain. For the local H1a genotype, no significant differences in adsorption capacity were observed among the isolates. However, the replication ability and cytopathic effect of the more recent H1a isolates, MV15-278(H1a) and MV16-189(H1a) from Fujian Province, China, showed a decline compared to earlier isolates, suggesting that under strong immune pressure, local strains may have mutated, slowing virus transmission and spread. This may eventually lead to the interruption of local virus circulation and the elimination of measles.
[1] Minta AA, Ferrari M, Antoni S, et al. Progress toward regional measles elimination-worldwide, 2000-2021[J]. MMWR Morb Mortal Wkly Rep, 2022, 71(47):1489-1495. DOI:10. 15585/mmwr. mm7147a1.
[2] Brown KE, Rota PA, Goodson JL, et al. Genetic characterization of measles and rubella viruses detected through global measles and rubella elimination surveillance, 2016-2018[J]. MMWR Morb Mortal Wkly Rep, 2019, 68(26):587-591. DOI:10. 15585/mmwr. mm6826a3.
[3] Wang H, Zhang Y, Mao N, et al. Molecular characterization of measles viruses in China:circulation dynamics of the endemic H1 genotype from 2011 to 2017[J]. PLoS One, 2019, 14(6):e0218782. DOI:10. 1371/journal. pone. 0218782.
[4] Wang H, Zhu Z, Duan X, et al. Transmission pattern of measles virus circulating in China during 1993-2021:genotyping evidence supports that China is approaching measles elimination[J]. Clin Infect Dis, 2023, 76(3):e1140-e1149. DOI:10. 1093/cid/ciac674.
[5] Dixon M, Ferrari M, Antoni S, et al. Progress toward regional measles elimination-worldwide, 2000–2020[J]. Morb Mortal Week Rep, 2021, 70(45):1563-1569. DOI:10. 15585/mmwr. mm7045a1.
[6] Kouomou DW, Nerrienet E, Mfoupouendoun J, et al.Measles virus strains circulating in central and west Africa:geographical Distribution of two B3 Genotypes[J]. J Med Virol, 2002, 68(3):433-440. DOI:10. 1002/jmv. 10222.
[7] Gouandjika-Vasilache I, Waku-Kouomou D, Ménard D, et al. Cocirculation of measles virus genotype B2 and B3. 1 in central african republic during the 2000 measles epidemic[J]. J Med Virol, 2006, 78(7):964-970.DOI:10. 1002/jmv. 20648.
[8] Scobie HM, Ilunga BK, Mulumba A, et al. Antecedent causes of a measles resurgence in the democratic republic of the congo[J]. Pan Afr Med J, 2015, 21:30. DOI:10. 11604/pamj. 2015. 21. 30. 6335.
[9] Dia N, Fall A, Ka R, et al. Epidemiology and genetic characterization of measles strains in senegal, 2004-2013[J]. PLoS One, 2015, 10(5):e0121704. DOI:10. 1371/journal. pone. 0121704.
[10]Duraisamy R, Rota PA, Palani G, et al. Molecular characterization of wild-type measles viruses in Tamil Nadu, India, during 2005-2006:Relationship of Genotype D8 Strains from Tamil Nadu to global strains[J]. J Med Virol, 2012, 84(2):348-357. DOI:10. 1002/jmv. 22244.
[11]Wairagkar N, Rota PA, Liffick S, et al.Characterization of measles sequences from pune, India[J]. J Med Virol, 2002, 68(4):611-614. DOI:10. 1002/jmv. 10228.
[12]Waku-Kouomou D, Landreau D, Olivier S, et al.Molecular characterization of measles virus circulating in the Indian Ocean Islands during 2005-2006 and in France in 2006[J]. J Med Virol, 2007, 79(9):1381-1387.DOI:10. 1002/jmv. 20959.
[13]WHO. Genetic characterization of measles and rubella viruses detected through surveillance for global measles and rubella elimination,2016–2018[J/OL]. Wkly Epidemiol Rec,2019,94:301–308. https：//apps. who.int/iris/bitstream/handle/10665/325738/WER9427-enfr. pdf?ua=1.
[14]Li S, Qian X, Yuan Z, et al. Molecular epidemiology of measles virus iInfection in Shanghai in 2000-2012:the first appearance of genotype D8[J]. Braz J Infect Dis,2014, 18(6):581-590. DOI:10. 1016/j.bjid. 2014. 05. 018.
[15]王淑蕾，李崇山，王慧玲，等.中国大陆首次发现输入性B3基因型麻疹病毒病例[J].病毒学报，2014, 30(5):535-540. DOI:10. 13242/j. cnki.bingduxuebao. 002544.
[16]Chen M, Zhang Y, Huang F, et al. Endemic and imported measles virus-associated outbreaks among adults, Beijing, China, 2013[J]. Emerg Infect Dis,2015, 21(3):477-479. DOI:10. 3201/eid2103. 140646.
[17]Deng X, Hu Y, Lu P, et al. The first outbreak of measles virus caused by imported genotype D8 in Jiangsu province of China[J]. Braz J Infect Dis, 2019,23(1):66-69. DOI:10. 1016/j. bjid. 2019. 02. 003.
[18]王慧玲，王淑蕾，任丽，等. 2013-2014年我国输入性B3基因型麻疹病毒的基因特征分析[J].病毒学报，2017, 33(4):577-582. DOI:10. 13242/j. cnki.bingduxuebao. 003185.
[19]李东，杨秀惠，张苏晗，等.福建省发现输入性B3基因型麻疹病毒[J].中华流行病学杂志，2020, 41(6):946-951. DOI:10. 3760/cma. j. cn112338-20190815-00599.
[20]李东，杨秀惠，张苏晗，等.福建省输入性D8基因型麻疹病毒分子流行病学特征[J].病毒学报，2021, 37(5):1066-1073. DOI:10. 13242/j. cnki.bingduxuebao. 004026.
[21]Bianchi S, Canuti M, Ciceri G, et al. Molecular epidemiology of B3 and D8 measles viruses through hemagglutinin phylogenetic history[J]. Int J Mol Sci,2020, 21(12):E4435. DOI:10. 3390/ijms21124435.
[22]Ackley SF, Hacker JK, Enanoria WTA, et al.Genotype-specific measles transmissibility:a branching process analysis[J]. Clin Infect Dis, 2018, 66(8):1270-1275. DOI:10. 1093/cid/cix974.
[23]Plumet S, Duprex WP, Gerlier D. Dynamics of Viral RNA Synthesis during Measles Vvirus Infection[J]. J Virol, 2005, 79(11):6900-6908. DOI:10. 1128/jvi. 79. 11. 6900-6908. 2005.
基本信息:
DOI：10.13242/j.cnki.bingduxuebao.004676
中图分类号:R373.11
引用信息:
[1]尤娜,张梦萍,张苏晗等.不同基因型麻疹病毒株细胞吸附力、复制力及致细胞病变能力的测定[J].病毒学报,2025,41(01):39-47.DOI:10.13242/j.cnki.bingduxuebao.004676.
基金信息:
福建省科技创新平台建设项目（项目号：2019Y2001）,题目：福建省预防医学研究院建设~~