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2024 03 v.40 476-483
穿心莲内酯调控p38及ERK磷酸化改善甲型流感病毒诱导肺上皮细胞损伤的作用
基金项目(Foundation):
邮箱(Email): lindu029@163.com;
DOI: 10.13242/j.cnki.bingduxuebao.004506
中文作者单位:

山东第一医科大学附属济南人民医院;

摘要(Abstract):

甲型流感病毒(Influenza A virus,IAV)感染引起肺上皮细胞损伤与促进p38磷酸化、抑制细胞外调节蛋白激酶(Extracellular regulated protein kinases,ERK)磷酸化有关,穿心莲内酯(Andrographolide,AD)通过抗凋亡、抗炎、抗氧化等途径发挥细胞保护作用且对促分裂素原活化蛋白激酶38(Protein 38,p38)、ERK的磷酸化具有调控作用。为研究AD通过调控p38及ERK磷酸化改善IAV诱导肺上皮细胞损伤的作用,本文培养小鼠肺上皮细胞株MLE-12,按照MOI=0.2感染IAV 2h后给予不同剂量(6.25、12.5、25.0 mg/L)AD或25.0 mg/L AD联合对照溶剂(体积分数0.1%)、p38激动剂SB202190(10μmol/L)、ERK抑制剂PD98059(20μmol/L)处理10h,检测细胞的增殖抑制率、凋亡率,培养基中白介素(Interleukin,IL)-1β、肿瘤坏死因子-α(Tumor necrosis factor-α,TNF-α)、IL-6、丙二醛(Malondialdehyde,MDA)、超氧化物歧化酶(Superoxide dismutase,SOD)、总抗氧化力(Total antioxidant capacity,T-AOC)的含量,细胞中p-p38、p-ERK的表达水平。结果显示:感染IAV的MLE-12细胞中SOD、T-AOC含量、pERK表达水平降低,增殖抑制率、凋亡率、IL-1β、TNF-α、IL-6、MDA含量及p-p38表达水平增加(P<0.05);不同剂量AD组MLE-12细胞中SOD、T-AOC含量、p-ERK表达水平增加,增殖抑制率、凋亡率、IL-1β、TNF-α、IL-6、MDA含量及p-p38表达水平降低(P<0.05);SB202190+AD组、PD98059+AD组MLE-12细胞中SOD、T-AOC含量降低,增殖抑制率、凋亡率、IL-1β、TNF-α、IL-6、MDA含量增加(P<0.05)。以上结果表明,AD通过抑制p38磷酸化、促进ERK磷酸化的方式抑制细胞凋亡、炎症反应及氧化应激反应,进而减轻IAV诱导肺上皮细胞损伤。

关键词(KeyWords): 甲型流感病毒;;肺上皮细胞;;穿心莲内酯;;p38;;细胞外调节蛋白激酶
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基本信息:

DOI:10.13242/j.cnki.bingduxuebao.004506

中图分类号:R285

引用信息:

[1]李刚,田甜,王磊等.穿心莲内酯调控p38及ERK磷酸化改善甲型流感病毒诱导肺上皮细胞损伤的作用[J].病毒学报,2024,40(03):476-483.DOI:10.13242/j.cnki.bingduxuebao.004506.

基金信息:

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