2020 03 v.36 493-501
2019新型冠状病毒的研究进展
基金项目(Foundation):
科技部重点研发计划项目新型冠状病毒感染的肺炎疫情应急(项目号:2020YFC0840900),题目:2019-nCoV基因组变异规律和流行趋势预测研究~~
邮箱(Email):
DOI:
10.13242/j.cnki.bingduxuebao.003677
中文作者单位:
中山大学公共卫生学院(深圳);
摘要(Abstract):
自2019年12月以来,新型冠状病毒SARS-CoV-2引发的肺炎疫情在中国武汉暴发,随后疫情迅速蔓延,扩散至中国其它各省市。新型冠状病毒属于β冠状病毒属,与SARS-CoV和MERS-CoV近缘但有较大区别,尤其是刺突蛋白(Spike,S),根据序列相似性推断可能来源于蝙蝠,使用与SARS-CoV相同的血管紧张素转化酶Ⅱ(Angiotensin-converting enzyme Ⅱ,ACE2)受体进入细胞,以飞沫和接触传播为主要传播途径,缺乏有效药物治疗和疫苗是疫情防控的挑战。
关键词(KeyWords):
新型冠状病毒(SARS-CoV-2);;血管紧张素转化酶Ⅱ(ACE2);;病毒进化起源
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参考文献
[1]U. S. CDC(2020). 2019 Novel Coronavirus(2019-nCoV),Wuhan,China[EB/OL]. https://www. cdc.gov/coronavirus/novel-coronavirus-2019.html.
[2]Wu F,Zhao S,Yu B,Chen Y M,Wang W,Song Z G,Hu Y,Tao Z W,Tian J H,Pei Y Y,Yuan M L,Zhang Y L,Dai F H,Liu Y,Wang Q M,Zheng J J,Xu L,Holmes E C,Zhang Y Z. A new coronavirus associated with human respiratory disease in China[J]. Nature,2020,579(7798):265–269.
[3]Zhou P,Yang X L,Wang X G,Hu B,Zhang L,Zhang W,Si H R,Zhu Y,Li B,Huang C L,Chen H D,Chen J,Luo Y,Guo H,Jiang R D,Liu M Q,Chen Y,Shen X R,Wang X,Zheng X S,Zhao K,Chen Q J,Deng F,Liu L L,Yan B,Zhan F X,Wang Y Y,Xiao G F,Shi Z L. A pneumonia outbreak associated with a new coronavirus of probable bat origin[J]. Nature,2020,579(7798):270-273.
[4]Zhu N,Zhang D,Wang W,Li X,Yang B,Song J,Zhao X,Huang B,Shi W,Lu R,Niu P,Zhan F,Ma X,Wang D,Xu W,Wu G,Gao G F,Tan W,China Novel Coronavirus I,Research T. A novel coronavirus from patients with pneumonia in China,2019[J]. N Engl J Med,2020,382(8):727-733.
[5]Ren L L,Wang Y M,Wu Z Q,Xiang Z C,Guo L,Xu T,Jiang Y Z,Xiong Y,Li Y J,Li X W,Li H,Fan G H,Gu X Y,Xiao Y,Gao H,Xu J Y,Yang F,Wang X M,Wu C,Chen L,Liu Y W,Liu B,Yang J,Wang X R,Dong J,Li L,Huang C L,Zhao J P,Hu Y,Cheng Z S,Liu L L,Qian Z H,Qin C,Jin Q,Cao B,Wang J W. Identification of a novel coronavirus causing severe pneumonia in human:a descriptive study[J].Chin Med J(Engl),2020,133(9):1015-1024.
[6]Gorbalenya A E,Baker S C,Baric R S,de Groot R J,Drosten C,Gulyaeva A A,Haagmans B L,Lauber C,Leontovich A M,Neuman B W,Penzar D,Perlman S,Poon L L M,Samborskiy D,Sidorov I A,Sola I,Ziebuhr J. Severe acute respiratory syndrome-related coronavirus:The species and its viruses-a statement of the Coronavirus Study Group[J/OL]. Nature Microbiology, 2020,5,5536-544; doi:10.1038/s41564-020-0695-z.
[7]Jiang S,Shi Z,Shu Y,Song J,Gao G F,Tan W,Guo D. A distinct name is needed for the new coronavirus[J]. Lancet,2020,395(10228):949. doi:10.1016/S0140-6736(20)30419-0
[8]Adams M J,Lefkowitz E J,King A M,Carstens E B.Ratification vote on taxonomic proposals to the International Committee on Taxonomy of Viruses(2014)[J].Arch Virol,2014,159(10):2831-2841.
[9]Xu X,Chen P,Wang J,Feng J,Zhou H,Li X,Zhong W,Hao P. Evolution of the novel coronavirus from the ongoing Wuhan outbreak and modeling of its spike protein for risk of human transmission[J]. Sci China Life Sci,2020,63(3),457-460.
[10]Chen Y,Liu Q,Guo D. Emerging coronaviruses:genome structure,replication,and pathogenesis[J]. J Med Virol,2020,92(4):418-423.
[11]Lu R,Zhao X,Li J,Niu P,Yang B,Wu H,Wang W,Song H,Huang B,Zhu N,Bi Y,Ma X,Zhan F,Wang L,Hu T,Zhou H,Hu Z,Zhou W,Zhao L,Chen J,Meng Y,Wang J,Lin Y,Yuan J,Xie Z,Ma J,Liu W J,Wang D,Xu W,Holmes E C,Gao G F,Wu G,Chen W,Shi W,Tan W. Genomic characterisation and epidemiology of 2019 novel coronavirus:implications for virus origins and receptor binding[J]. Lancet,2020,395(10224),565-574.
[12]Chafekar A,Fielding B C. MERS-CoV:understanding the latest human coronavirus threat[J]. Viruses,2018,10(2):93. doi:10.3390/v10020093
[13]Zaki A M,van Boheemen S,Bestebroer T M,Osterhaus A D M E,Fouchier R A M. Isolation of a novel coronavirus from a man with pneumonia in Saudi Arabia[J]. N Engl J Med,2012,367(19):1814-1820.
[14]Ksiazek T G,Erdman D,Goldsmith C S,Zaki S R,Peret T,Emery S,Tong S,Urbani C,Comer J A,Lim W,Rollin P E,Dowell S F,Ling A-E,Humphrey C D,Shieh W-J,Guarner J,Paddock C D,Rota P,Fields B,DeRisi J,Yang J-Y,Cox N,Hughes J M,LeDuc J W,Bellini W J,Anderson L J,Group S W. A novel coronavirus associated with severe acute respiratory syndrome[J]. N Engl J Med,2003,348(20):1953-1966.
[15]Li Q,Guan X,Wu P,Wang X,Zhou L,Tong Y,Ren R,Leung K S M,Lau E H Y,Wong J Y,Xing X,Xiang N,Wu Y,Li C,Chen Q,Li D,Liu T,Zhao J,Li M,Tu W,Chen C,Jin L,Yang R,Wang Q,Zhou S,Wang R,Liu H,Luo Y,Liu Y,Shao G,Li H,Tao Z,Yang Y,Deng Z,Liu B,Ma Z,Zhang Y,Shi G,Lam T T Y,Wu J T K,Gao G F,Cowling B J,Yang B,Leung G M,Feng Z. Early transmission dynamics in Wuhan,China,of novel coronavirus-infected pneumonia[J]. N Engl J Med,2020,382(13):1199-1207.
[16]Chen N,Zhou M,Dong X,Qu J,Gong F,Han Y,Qiu Y,Wang J,Liu Y,Wei Y,Xia J a,Yu T,Zhang X,Zhang L. Epidemiological and clinical characteristics of 99 cases of 2019 novel coronavirus pneumonia in Wuhan,China:a descriptive study[J]. Lancet,2020,395(10223):507-513.
[17]Wong M C,Javornik Cregeen S J,Ajami N J,Petrosino J F. Evidence of recombination in coronaviruses implicating pangolin origins of nCoV-2019[J/OL]. bioRxiv 2020.02.07.939207;doi:https://doi.org/10.1101/2020.02.07.939207
[18]Lam T T Y,Shum M H H,Zhu H C,Tong Y G,Ni X B,Liao Y S,Wei W,Cheung W Y M,Li W J,Li L F,Leung G M,Holmes E C,Hu Y L,Guan Y. Identification of 2019-nCoV related coronaviruses in Malayan pangolins in southern China[J/OL]. Nature. doi:https://doi.org/10.1038/s41586-020-2169-0(2020)
[19]Liu P,Chen W,Chen J P. Viral metagenomics revealed Sendai virus and coronavirus infection of Malayan Pangolins(Manis javanica)[J]. Viruses,2019,11(11):979.doi:10.3390/v11110979
[20]Xiao K,Zhai J,Feng Y,Zhou N,Zhang X,Zou J J,Li N,Guo Y,Li X,Shen X,Zhang Z,Shu F,Huang W,Li Y,Zhang Z,Chen R A,Wu Y J,Peng S M,Huang M,Xie W J,Cai Q H,Hou F H,Liu Y,Chen W,Xiao L,Shen Y. Isolation and characterization of2019-nCoV-like coronavirus from Malayan Pangolins[J/OL]. Nature. doi:https://doi.org/10.1038/s41586-020-2313-x(2020).
[21]Wu A,Peng Y,Huang B,Ding X,Wang X,Niu P,Meng J,Zhu Z,Zhang Z,Wang J,Sheng J,Quan L,Xia Z,Tan W,Cheng G,Jiang T. Genome Composition and divergence of the novel coronavirus(2019-nCoV)originating in China[J]. Cell Host Microbe,2020,27(3),325-328.
[22]van Boheemen S,de Graaf M,Lauber C,Bestebroer T M,Raj V S,Zaki A M,Osterhaus A D,Haagmans B L,Gorbalenya A E,Snijder E J,Fouchier R A. Genomic characterization of a newly discovered coronavirus associated with acute respiratory distress syndrome in humans[J/OL]. mBio,2012,3(6):e00473-12.
[23]Li F. Structure,function,and evolution of coronavirus spike proteins[J]. Annu Rev Virol, 2016, 3(1):237-261.
[24]Benvenuto D,Giovanetti M,Ciccozzi A,Spoto S,Angeletti S,Ciccozzi M. The 2019-new coronavirus epidemic:evidence for virus evolution[J]. J Med Virol,2020,92(4):455-459
[25]Lu G,Hu Y,Wang Q,Qi J,Gao F,Li Y,Zhang Y,Zhang W,Yuan Y,Bao J,Zhang B,Shi Y,Yan J,Gao G F. Molecular basis of binding between novel human coronavirus MERS-CoV and its receptor CD26[J].Nature. 2013,500(7461):227-231.
[26]Pallesen J,Wang N,Corbett K S,Wrapp D,Kirchdoerfer R N,Turner H L,Cottrell C A,Becker M M,Wang L,Shi W,Kong W P,Andres E L,Kettenbach A N,Denison M R,Chappell J D,Graham B S,Ward A B,McLellan J S. Immunogenicity and structures of a rationally designed prefusion MERS-CoV spike antigen[J/OL]. Proc Natl Acad Sci U S A,2017,114(35):E7348-E7357.
[27]Song W,Gui M,Wang X,Xiang Y. Cryo-EM structure of the SARS coronavirus spike glycoprotein in complex with its host cell receptor ACE2[J/OL]. PLoS Pathog,2018,14(8):e1007236.
[28]Li F. Receptor recognition and cross-species infections of SARS coronavirus[J]. Antiviral Res,2013,100(1):246-254.
[29]Coutard B,Valle C,de Lamballerie X,Canard B,Seidah N G,Decroly E. The spike glycoprotein of the new coronavirus 2019-nCoV contains a furin-like cleavage site absent in CoV of the same clade[J]. Antiviral Res,2020,176:104742.
[30]Wrapp D,Wang N,Corbett K S,Goldsmith J A,Hsieh C-L,Abiona O,Graham B S,McLellan J S.Cryo-EM structure of the 2019-nCoV spike in the prefusion conformation[J]. Science,2020,367(6483):1260-1263. doi:10.1126/science.abb2507
[31]Dong N,Yang X,Ye L,Chen K,Chan E W C,Yang M,Chen S. Genomic and protein structure modelling analysis depicts the origin and infectivity of 2019-nCoV,a new coronavirus which caused a pneumonia outbreak in Wuhan,China[J].bioRxiv 2020.01.20.913368;doi:https://doi. org/10.1101/2020.01.20.913368
[32]Cui L,Wang H,Ji Y,Yang J,Xu S,Huang X,Wang Z,Qin L,Tien P,Zhou X,Guo D,Chen Y. The nucleocapsid protein of coronaviruses acts as a viral suppressor of RNA silencing in mammalian cells[J]. J Virol,2015,89(17):9029-9043.
[33]Schoeman D,Fielding B C. Coronavirus envelope protein:current knowledge[J]. Virol J,2019,16(1):69.doi:10.1186/s12985-019-1182-0.
[34]Siu K L,Chan C P,Kok K H,Chiu-Yat Woo P,Jin D Y. Suppression of innate antiviral response by severe acute respiratory syndrome coronavirus M protein is mediated through the first transmembrane domain[J]. Cell Mol Immunol,2014,11(2):141-149.
[35]Keum Y S,Jeong Y J. Development of chemical inhibitors of the SARS coronavirus:viral helicase as a potential target[J]. Biochem Pharmacol,2012,84(10):1351-1358.
[36]Huang C,Lokugamage K G,Rozovics J M,Narayanan K,Semler B L,Makino S. SARS coronavirus nsp1 protein induces template-dependent endonucleolytic cleavage of mRNAs:viral mRNAs are resistant to nsp1-induced RNA cleavage[J/OL]. PLoS pathogens,2011,7(12):e1002433-e1002433.
[37]Tanaka T,Kamitani W,DeDiego M L,Enjuanes L,Matsuura Y. Severe acute respiratory syndrome coronavirus nsp1 facilitates efficient propagation in cells through a specific translational shutoff of host mRNA[J]. J Virol,2012,86(20):11128-11137.
[38]Lei J,Kusov Y,Hilgenfeld R. Nsp3 of coronaviruses:structures and functions of a large multi-domain protein[J]. Antivir Res,2018,149:58-74.
[39]Beachboard D C,Anderson-Daniels J M,Denison M R.Mutations across murine hepatitis virus nsp4 alter virus fitness and membrane modifications[J]. J Virol,2015,89(4):2080-2089.
[40]Stobart C C,Sexton N R,Munjal H,Lu X,Molland K L,Tomar S,Mesecar A D,Denison M R. Chimeric exchange of coronavirus nsp5 proteases(3CLpro)identifies common and divergent regulatory determinants of protease activity[J]. J Virol,2013,87(23):12611-12618.
[41]Cottam E M,Whelband M C,Wileman T. Coronavirus NSP6 restricts autophagosome expansion[J]. Autophagy,2014,10(8):1426-1441.
[42]Kirchdoerfer R N,Ward A B. Structure of the SARSCoV nsp12 polymerase bound to nsp7 and nsp8 co-factors[J]. Nat Commun,2019,10(1):2342-2342.
[43]Zeng Z,Deng F,Shi K,Ye G,Wang G,Fang L,Xiao S,Fu Z,Peng G. Dimerization of coronavirus nsp9 with diverse modes enhances its nucleic acid binding affinity[J/OL]. J Virol,2018,92(17):e00692-00618.
[44]Bouvet M,Lugari A,Posthuma C C,Zevenhoven J C,Bernard S,Betzi S,Imbert I,Canard B,Guillemot JC,Lécine P,Pfefferle S,Drosten C,Snijder E J,Decroly E,Morelli X. Coronavirus Nsp10,a critical co-factor for activation of multiple replicative enzymes[J]. J Biol Chem,2014,289(37):25783-25796.
[45]Jia Z,Yan L,Ren Z,Wu L,Wang J,Guo J,Zheng L,Ming Z,Zhang L,Lou Z,Rao Z. Delicate structural coordination of the severe acute respiratory syndrome coronavirus Nsp13 upon ATP hydrolysis[J]. Nucleic acids research,2019,47(12):6538-6550.
[46]Ma Y,Wu L,Shaw N,Gao Y,Wang J,Sun Y,Lou Z,Yan L,Zhang R,Rao Z. Structural basis and functional analysis of the SARS coronavirus nsp14-nsp10complex[J]. Proc Natl Acad Sci U S A,2015,112(30):9436-9441.
[47]Zhang L,Li L,Yan L,Ming Z,Jia Z,Lou Z,Rao Z.Structural and biochemical characterization of endoribonuclease nsp15 encoded by Middle East respiratory syndrome coronavirus[J/OL]. J Virol,2018,92(22):e00893-00818.
[48]Shi P,Su Y,Li R,Liang Z,Dong S,Huang J. PEDV nsp16 negatively regulates innate immunity to promote viral proliferation[J]. Virus Res,2019,265:57-66.
[49]Tan Y W,Fung T S,Shen H,Huang M,Liu D X.Coronavirus infectious bronchitis virus non-structural proteins 8 and 12 form stable complex independent of the non-translated regions of viral RNA and other viral proteins[J]. Virology,2018,513:75-84.
[50]国卫办关于印发新型冠状病毒肺炎诊疗方案(试行第六版)的通知.新型冠状病毒肺炎诊疗方案(试行第六版)http://www. nhc. gov. cn/xcs/zhengcwj/202002/8334a8326dd94d329df351d7da8aefc2.shtml
[51]新型冠状病毒国家科技资源服务系统. http://nmdc.cn/#/nCoV
[52]新型冠状病毒防控方案(第五版).国家卫生健康委办公厅关于印发新型冠状病毒肺炎防控方案(第五版)的通知,http://www.nhc.gov.cn/xcs/zhengcwj/202002/a5d6f7b8c48c451c87dba14889b30147.shtml
[53]Real-Time RT-PCR Panel for Detection 2019-Novel Coronavirus[EB/OL]. www.cdc.gov.
[54]Corman V M,Landt O,Kaiser M,Molenkamp R,Meijer A,Chu D K,Bleicker T,Brunink S,Schneider J,Schmidt M L,Mulders D G,Haagmans B L,van der Veer B,van den Brink S,Wijsman L,Goderski G,Romette J L,Ellis J,Zambon M,Peiris M,Goossens H,Reusken C,Koopmans M P,Drosten C. Detection of 2019 novel coronavirus(2019-nCoV)by real-time RT-PCR[J]. Euro Surveill,2020,25(3):2000045.
[55]Chu D K W,Pan Y,Cheng S M S,Hui K P Y,Krishnan P,Liu Y,Ng D Y M,Wan C K C,Yang P,Wang Q,Peiris M,Poon L L M. Molecular diagnosis of a novel coronavirus(2019-nCoV)causing an outbreak of pneumonia[J]. Clin Chem,2020,66(4):549-555.
[56]Zhang F. A protocol for detection of COVID-19 using CRISPR diagnostics. 2020.
[57]钟南山院士指导研发快速检测试剂盒采一滴血15分钟内可出结果[EB/OL]. http://news.cctv.com/2020/02/15/ARTIj8iCzJahoFhLELNYqtya200215.shtml
[58]葛均波院士团队联合研发新型冠状病毒IgM抗体快检试剂盒通过注册检验[EB/OL]. http://www.zs-hospital.sh.cn/zsyy/n33/n66/u1ai4804.html.
[59]15分钟快速检测南开大学团队研获新冠病毒抗体检测试剂盒[EB/OL]. http://news. nankai. edu. cn/ywsd/system/2020/02/15/030037569.shtml
[60]华西医院科技联合攻关团队成功研发多款新型冠状病毒检测试剂盒[EB/OL]. http://www.wchscu.cn/comprehensive/50848. html? from=timeline&isappinstalled=0
[61]Zhang N,Wang L,Deng X,Liang R,Su M,He C,Hu L,Su Y,Ren J,Yu F,Du L,Jiang S. Recent advances in the detection of respiratory virus infection in humans[J]. J Med Virol,2020,92(4):408-417.
[62]Chen W,Lan Y,Yuan X,Deng X,Li Y,Cai X,Li L,He R,Tan Y,Deng X,Gao M,Tang G,Zhao L,Wang J,Fan Q,Wen C,Tong Y,Tang Y,Hu F,Li F,Tang X. Detectable 2019-nCoV viral RNA in blood is a strong indicator for the further clinical severity[J].Emerg Microbes Infect,2020,9(1):469-473.
[63]Han Y,Yang H. The transmission and diagnosis of2019 novel coronavirus infection disease(COVID-19):a Chinese perspective[J]. J Med Virol, 2020. doi:10.1002/jmv.25749
[64]Zhang J,Liu J,Li N,Liu Y,Ye R,Qin X,Zheng R.Serological detection of 2019-nCoV respond to the epidemic:a useful complement to nucleic acid testing[J/OL]. medRxiv 2020.03.04.20030916;doi:https://doi.org/10.1101/2020.03.04.20030916
[65]钟南山院士公布最新研究成果,有两个好消息[EB/OL]. https://www. takefoto. cn/viewnews-2073971. html
[66]中国政府网.关于新冠肺炎治疗药物、疫苗、病毒宿主,有这些最新消息[EB/OL]. http://www.gov.cn/fuwu/2020-02/15/content_5479428.html
[67]Li H,Wang Y M,Xu J Y,Cao B. Potential antiviral therapeutics for 2019 novel coronavirus[J]. Zhonghua Jie He He Hu Xi Za Zhi,2020,43(3):170-172.
[68]Lu H. Drug treatment options for the 2019-new coronavirus(2019-nCoV)[J]. Biosci Trends,2020,14(1):69-71.
[69]Richardson P,Griffin I,Tucker C,Smith D,Oechsle O,Phelan A,Stebbing J. Baricitinib as potential treatment for 2019-nCoV acute respiratory disease[J/OL].Lancet,2020,395(10223):e30-e31.
[70]Wang M,Cao R,Zhang L,Yang X,Liu J,Xu M,Shi Z,Hu Z,Zhong W,Xiao G. Remdesivir and chloroquine effectively inhibit the recently emerged novel coronavirus(2019-nCoV)in vitro[J]. Cell Res,2020,30(3):269-271.
[71]湖北省新型冠状病毒肺炎疫情防控工作指挥部新闻发布会[2020年2月13日][EB/OL]. http://www.chinanews.com/shipin/spfts/20200217/2558.shtml
[2]Wu F,Zhao S,Yu B,Chen Y M,Wang W,Song Z G,Hu Y,Tao Z W,Tian J H,Pei Y Y,Yuan M L,Zhang Y L,Dai F H,Liu Y,Wang Q M,Zheng J J,Xu L,Holmes E C,Zhang Y Z. A new coronavirus associated with human respiratory disease in China[J]. Nature,2020,579(7798):265–269.
[3]Zhou P,Yang X L,Wang X G,Hu B,Zhang L,Zhang W,Si H R,Zhu Y,Li B,Huang C L,Chen H D,Chen J,Luo Y,Guo H,Jiang R D,Liu M Q,Chen Y,Shen X R,Wang X,Zheng X S,Zhao K,Chen Q J,Deng F,Liu L L,Yan B,Zhan F X,Wang Y Y,Xiao G F,Shi Z L. A pneumonia outbreak associated with a new coronavirus of probable bat origin[J]. Nature,2020,579(7798):270-273.
[4]Zhu N,Zhang D,Wang W,Li X,Yang B,Song J,Zhao X,Huang B,Shi W,Lu R,Niu P,Zhan F,Ma X,Wang D,Xu W,Wu G,Gao G F,Tan W,China Novel Coronavirus I,Research T. A novel coronavirus from patients with pneumonia in China,2019[J]. N Engl J Med,2020,382(8):727-733.
[5]Ren L L,Wang Y M,Wu Z Q,Xiang Z C,Guo L,Xu T,Jiang Y Z,Xiong Y,Li Y J,Li X W,Li H,Fan G H,Gu X Y,Xiao Y,Gao H,Xu J Y,Yang F,Wang X M,Wu C,Chen L,Liu Y W,Liu B,Yang J,Wang X R,Dong J,Li L,Huang C L,Zhao J P,Hu Y,Cheng Z S,Liu L L,Qian Z H,Qin C,Jin Q,Cao B,Wang J W. Identification of a novel coronavirus causing severe pneumonia in human:a descriptive study[J].Chin Med J(Engl),2020,133(9):1015-1024.
[6]Gorbalenya A E,Baker S C,Baric R S,de Groot R J,Drosten C,Gulyaeva A A,Haagmans B L,Lauber C,Leontovich A M,Neuman B W,Penzar D,Perlman S,Poon L L M,Samborskiy D,Sidorov I A,Sola I,Ziebuhr J. Severe acute respiratory syndrome-related coronavirus:The species and its viruses-a statement of the Coronavirus Study Group[J/OL]. Nature Microbiology, 2020,5,5536-544; doi:10.1038/s41564-020-0695-z.
[7]Jiang S,Shi Z,Shu Y,Song J,Gao G F,Tan W,Guo D. A distinct name is needed for the new coronavirus[J]. Lancet,2020,395(10228):949. doi:10.1016/S0140-6736(20)30419-0
[8]Adams M J,Lefkowitz E J,King A M,Carstens E B.Ratification vote on taxonomic proposals to the International Committee on Taxonomy of Viruses(2014)[J].Arch Virol,2014,159(10):2831-2841.
[9]Xu X,Chen P,Wang J,Feng J,Zhou H,Li X,Zhong W,Hao P. Evolution of the novel coronavirus from the ongoing Wuhan outbreak and modeling of its spike protein for risk of human transmission[J]. Sci China Life Sci,2020,63(3),457-460.
[10]Chen Y,Liu Q,Guo D. Emerging coronaviruses:genome structure,replication,and pathogenesis[J]. J Med Virol,2020,92(4):418-423.
[11]Lu R,Zhao X,Li J,Niu P,Yang B,Wu H,Wang W,Song H,Huang B,Zhu N,Bi Y,Ma X,Zhan F,Wang L,Hu T,Zhou H,Hu Z,Zhou W,Zhao L,Chen J,Meng Y,Wang J,Lin Y,Yuan J,Xie Z,Ma J,Liu W J,Wang D,Xu W,Holmes E C,Gao G F,Wu G,Chen W,Shi W,Tan W. Genomic characterisation and epidemiology of 2019 novel coronavirus:implications for virus origins and receptor binding[J]. Lancet,2020,395(10224),565-574.
[12]Chafekar A,Fielding B C. MERS-CoV:understanding the latest human coronavirus threat[J]. Viruses,2018,10(2):93. doi:10.3390/v10020093
[13]Zaki A M,van Boheemen S,Bestebroer T M,Osterhaus A D M E,Fouchier R A M. Isolation of a novel coronavirus from a man with pneumonia in Saudi Arabia[J]. N Engl J Med,2012,367(19):1814-1820.
[14]Ksiazek T G,Erdman D,Goldsmith C S,Zaki S R,Peret T,Emery S,Tong S,Urbani C,Comer J A,Lim W,Rollin P E,Dowell S F,Ling A-E,Humphrey C D,Shieh W-J,Guarner J,Paddock C D,Rota P,Fields B,DeRisi J,Yang J-Y,Cox N,Hughes J M,LeDuc J W,Bellini W J,Anderson L J,Group S W. A novel coronavirus associated with severe acute respiratory syndrome[J]. N Engl J Med,2003,348(20):1953-1966.
[15]Li Q,Guan X,Wu P,Wang X,Zhou L,Tong Y,Ren R,Leung K S M,Lau E H Y,Wong J Y,Xing X,Xiang N,Wu Y,Li C,Chen Q,Li D,Liu T,Zhao J,Li M,Tu W,Chen C,Jin L,Yang R,Wang Q,Zhou S,Wang R,Liu H,Luo Y,Liu Y,Shao G,Li H,Tao Z,Yang Y,Deng Z,Liu B,Ma Z,Zhang Y,Shi G,Lam T T Y,Wu J T K,Gao G F,Cowling B J,Yang B,Leung G M,Feng Z. Early transmission dynamics in Wuhan,China,of novel coronavirus-infected pneumonia[J]. N Engl J Med,2020,382(13):1199-1207.
[16]Chen N,Zhou M,Dong X,Qu J,Gong F,Han Y,Qiu Y,Wang J,Liu Y,Wei Y,Xia J a,Yu T,Zhang X,Zhang L. Epidemiological and clinical characteristics of 99 cases of 2019 novel coronavirus pneumonia in Wuhan,China:a descriptive study[J]. Lancet,2020,395(10223):507-513.
[17]Wong M C,Javornik Cregeen S J,Ajami N J,Petrosino J F. Evidence of recombination in coronaviruses implicating pangolin origins of nCoV-2019[J/OL]. bioRxiv 2020.02.07.939207;doi:https://doi.org/10.1101/2020.02.07.939207
[18]Lam T T Y,Shum M H H,Zhu H C,Tong Y G,Ni X B,Liao Y S,Wei W,Cheung W Y M,Li W J,Li L F,Leung G M,Holmes E C,Hu Y L,Guan Y. Identification of 2019-nCoV related coronaviruses in Malayan pangolins in southern China[J/OL]. Nature. doi:https://doi.org/10.1038/s41586-020-2169-0(2020)
[19]Liu P,Chen W,Chen J P. Viral metagenomics revealed Sendai virus and coronavirus infection of Malayan Pangolins(Manis javanica)[J]. Viruses,2019,11(11):979.doi:10.3390/v11110979
[20]Xiao K,Zhai J,Feng Y,Zhou N,Zhang X,Zou J J,Li N,Guo Y,Li X,Shen X,Zhang Z,Shu F,Huang W,Li Y,Zhang Z,Chen R A,Wu Y J,Peng S M,Huang M,Xie W J,Cai Q H,Hou F H,Liu Y,Chen W,Xiao L,Shen Y. Isolation and characterization of2019-nCoV-like coronavirus from Malayan Pangolins[J/OL]. Nature. doi:https://doi.org/10.1038/s41586-020-2313-x(2020).
[21]Wu A,Peng Y,Huang B,Ding X,Wang X,Niu P,Meng J,Zhu Z,Zhang Z,Wang J,Sheng J,Quan L,Xia Z,Tan W,Cheng G,Jiang T. Genome Composition and divergence of the novel coronavirus(2019-nCoV)originating in China[J]. Cell Host Microbe,2020,27(3),325-328.
[22]van Boheemen S,de Graaf M,Lauber C,Bestebroer T M,Raj V S,Zaki A M,Osterhaus A D,Haagmans B L,Gorbalenya A E,Snijder E J,Fouchier R A. Genomic characterization of a newly discovered coronavirus associated with acute respiratory distress syndrome in humans[J/OL]. mBio,2012,3(6):e00473-12.
[23]Li F. Structure,function,and evolution of coronavirus spike proteins[J]. Annu Rev Virol, 2016, 3(1):237-261.
[24]Benvenuto D,Giovanetti M,Ciccozzi A,Spoto S,Angeletti S,Ciccozzi M. The 2019-new coronavirus epidemic:evidence for virus evolution[J]. J Med Virol,2020,92(4):455-459
[25]Lu G,Hu Y,Wang Q,Qi J,Gao F,Li Y,Zhang Y,Zhang W,Yuan Y,Bao J,Zhang B,Shi Y,Yan J,Gao G F. Molecular basis of binding between novel human coronavirus MERS-CoV and its receptor CD26[J].Nature. 2013,500(7461):227-231.
[26]Pallesen J,Wang N,Corbett K S,Wrapp D,Kirchdoerfer R N,Turner H L,Cottrell C A,Becker M M,Wang L,Shi W,Kong W P,Andres E L,Kettenbach A N,Denison M R,Chappell J D,Graham B S,Ward A B,McLellan J S. Immunogenicity and structures of a rationally designed prefusion MERS-CoV spike antigen[J/OL]. Proc Natl Acad Sci U S A,2017,114(35):E7348-E7357.
[27]Song W,Gui M,Wang X,Xiang Y. Cryo-EM structure of the SARS coronavirus spike glycoprotein in complex with its host cell receptor ACE2[J/OL]. PLoS Pathog,2018,14(8):e1007236.
[28]Li F. Receptor recognition and cross-species infections of SARS coronavirus[J]. Antiviral Res,2013,100(1):246-254.
[29]Coutard B,Valle C,de Lamballerie X,Canard B,Seidah N G,Decroly E. The spike glycoprotein of the new coronavirus 2019-nCoV contains a furin-like cleavage site absent in CoV of the same clade[J]. Antiviral Res,2020,176:104742.
[30]Wrapp D,Wang N,Corbett K S,Goldsmith J A,Hsieh C-L,Abiona O,Graham B S,McLellan J S.Cryo-EM structure of the 2019-nCoV spike in the prefusion conformation[J]. Science,2020,367(6483):1260-1263. doi:10.1126/science.abb2507
[31]Dong N,Yang X,Ye L,Chen K,Chan E W C,Yang M,Chen S. Genomic and protein structure modelling analysis depicts the origin and infectivity of 2019-nCoV,a new coronavirus which caused a pneumonia outbreak in Wuhan,China[J].bioRxiv 2020.01.20.913368;doi:https://doi. org/10.1101/2020.01.20.913368
[32]Cui L,Wang H,Ji Y,Yang J,Xu S,Huang X,Wang Z,Qin L,Tien P,Zhou X,Guo D,Chen Y. The nucleocapsid protein of coronaviruses acts as a viral suppressor of RNA silencing in mammalian cells[J]. J Virol,2015,89(17):9029-9043.
[33]Schoeman D,Fielding B C. Coronavirus envelope protein:current knowledge[J]. Virol J,2019,16(1):69.doi:10.1186/s12985-019-1182-0.
[34]Siu K L,Chan C P,Kok K H,Chiu-Yat Woo P,Jin D Y. Suppression of innate antiviral response by severe acute respiratory syndrome coronavirus M protein is mediated through the first transmembrane domain[J]. Cell Mol Immunol,2014,11(2):141-149.
[35]Keum Y S,Jeong Y J. Development of chemical inhibitors of the SARS coronavirus:viral helicase as a potential target[J]. Biochem Pharmacol,2012,84(10):1351-1358.
[36]Huang C,Lokugamage K G,Rozovics J M,Narayanan K,Semler B L,Makino S. SARS coronavirus nsp1 protein induces template-dependent endonucleolytic cleavage of mRNAs:viral mRNAs are resistant to nsp1-induced RNA cleavage[J/OL]. PLoS pathogens,2011,7(12):e1002433-e1002433.
[37]Tanaka T,Kamitani W,DeDiego M L,Enjuanes L,Matsuura Y. Severe acute respiratory syndrome coronavirus nsp1 facilitates efficient propagation in cells through a specific translational shutoff of host mRNA[J]. J Virol,2012,86(20):11128-11137.
[38]Lei J,Kusov Y,Hilgenfeld R. Nsp3 of coronaviruses:structures and functions of a large multi-domain protein[J]. Antivir Res,2018,149:58-74.
[39]Beachboard D C,Anderson-Daniels J M,Denison M R.Mutations across murine hepatitis virus nsp4 alter virus fitness and membrane modifications[J]. J Virol,2015,89(4):2080-2089.
[40]Stobart C C,Sexton N R,Munjal H,Lu X,Molland K L,Tomar S,Mesecar A D,Denison M R. Chimeric exchange of coronavirus nsp5 proteases(3CLpro)identifies common and divergent regulatory determinants of protease activity[J]. J Virol,2013,87(23):12611-12618.
[41]Cottam E M,Whelband M C,Wileman T. Coronavirus NSP6 restricts autophagosome expansion[J]. Autophagy,2014,10(8):1426-1441.
[42]Kirchdoerfer R N,Ward A B. Structure of the SARSCoV nsp12 polymerase bound to nsp7 and nsp8 co-factors[J]. Nat Commun,2019,10(1):2342-2342.
[43]Zeng Z,Deng F,Shi K,Ye G,Wang G,Fang L,Xiao S,Fu Z,Peng G. Dimerization of coronavirus nsp9 with diverse modes enhances its nucleic acid binding affinity[J/OL]. J Virol,2018,92(17):e00692-00618.
[44]Bouvet M,Lugari A,Posthuma C C,Zevenhoven J C,Bernard S,Betzi S,Imbert I,Canard B,Guillemot JC,Lécine P,Pfefferle S,Drosten C,Snijder E J,Decroly E,Morelli X. Coronavirus Nsp10,a critical co-factor for activation of multiple replicative enzymes[J]. J Biol Chem,2014,289(37):25783-25796.
[45]Jia Z,Yan L,Ren Z,Wu L,Wang J,Guo J,Zheng L,Ming Z,Zhang L,Lou Z,Rao Z. Delicate structural coordination of the severe acute respiratory syndrome coronavirus Nsp13 upon ATP hydrolysis[J]. Nucleic acids research,2019,47(12):6538-6550.
[46]Ma Y,Wu L,Shaw N,Gao Y,Wang J,Sun Y,Lou Z,Yan L,Zhang R,Rao Z. Structural basis and functional analysis of the SARS coronavirus nsp14-nsp10complex[J]. Proc Natl Acad Sci U S A,2015,112(30):9436-9441.
[47]Zhang L,Li L,Yan L,Ming Z,Jia Z,Lou Z,Rao Z.Structural and biochemical characterization of endoribonuclease nsp15 encoded by Middle East respiratory syndrome coronavirus[J/OL]. J Virol,2018,92(22):e00893-00818.
[48]Shi P,Su Y,Li R,Liang Z,Dong S,Huang J. PEDV nsp16 negatively regulates innate immunity to promote viral proliferation[J]. Virus Res,2019,265:57-66.
[49]Tan Y W,Fung T S,Shen H,Huang M,Liu D X.Coronavirus infectious bronchitis virus non-structural proteins 8 and 12 form stable complex independent of the non-translated regions of viral RNA and other viral proteins[J]. Virology,2018,513:75-84.
[50]国卫办关于印发新型冠状病毒肺炎诊疗方案(试行第六版)的通知.新型冠状病毒肺炎诊疗方案(试行第六版)http://www. nhc. gov. cn/xcs/zhengcwj/202002/8334a8326dd94d329df351d7da8aefc2.shtml
[51]新型冠状病毒国家科技资源服务系统. http://nmdc.cn/#/nCoV
[52]新型冠状病毒防控方案(第五版).国家卫生健康委办公厅关于印发新型冠状病毒肺炎防控方案(第五版)的通知,http://www.nhc.gov.cn/xcs/zhengcwj/202002/a5d6f7b8c48c451c87dba14889b30147.shtml
[53]Real-Time RT-PCR Panel for Detection 2019-Novel Coronavirus[EB/OL]. www.cdc.gov.
[54]Corman V M,Landt O,Kaiser M,Molenkamp R,Meijer A,Chu D K,Bleicker T,Brunink S,Schneider J,Schmidt M L,Mulders D G,Haagmans B L,van der Veer B,van den Brink S,Wijsman L,Goderski G,Romette J L,Ellis J,Zambon M,Peiris M,Goossens H,Reusken C,Koopmans M P,Drosten C. Detection of 2019 novel coronavirus(2019-nCoV)by real-time RT-PCR[J]. Euro Surveill,2020,25(3):2000045.
[55]Chu D K W,Pan Y,Cheng S M S,Hui K P Y,Krishnan P,Liu Y,Ng D Y M,Wan C K C,Yang P,Wang Q,Peiris M,Poon L L M. Molecular diagnosis of a novel coronavirus(2019-nCoV)causing an outbreak of pneumonia[J]. Clin Chem,2020,66(4):549-555.
[56]Zhang F. A protocol for detection of COVID-19 using CRISPR diagnostics. 2020.
[57]钟南山院士指导研发快速检测试剂盒采一滴血15分钟内可出结果[EB/OL]. http://news.cctv.com/2020/02/15/ARTIj8iCzJahoFhLELNYqtya200215.shtml
[58]葛均波院士团队联合研发新型冠状病毒IgM抗体快检试剂盒通过注册检验[EB/OL]. http://www.zs-hospital.sh.cn/zsyy/n33/n66/u1ai4804.html.
[59]15分钟快速检测南开大学团队研获新冠病毒抗体检测试剂盒[EB/OL]. http://news. nankai. edu. cn/ywsd/system/2020/02/15/030037569.shtml
[60]华西医院科技联合攻关团队成功研发多款新型冠状病毒检测试剂盒[EB/OL]. http://www.wchscu.cn/comprehensive/50848. html? from=timeline&isappinstalled=0
[61]Zhang N,Wang L,Deng X,Liang R,Su M,He C,Hu L,Su Y,Ren J,Yu F,Du L,Jiang S. Recent advances in the detection of respiratory virus infection in humans[J]. J Med Virol,2020,92(4):408-417.
[62]Chen W,Lan Y,Yuan X,Deng X,Li Y,Cai X,Li L,He R,Tan Y,Deng X,Gao M,Tang G,Zhao L,Wang J,Fan Q,Wen C,Tong Y,Tang Y,Hu F,Li F,Tang X. Detectable 2019-nCoV viral RNA in blood is a strong indicator for the further clinical severity[J].Emerg Microbes Infect,2020,9(1):469-473.
[63]Han Y,Yang H. The transmission and diagnosis of2019 novel coronavirus infection disease(COVID-19):a Chinese perspective[J]. J Med Virol, 2020. doi:10.1002/jmv.25749
[64]Zhang J,Liu J,Li N,Liu Y,Ye R,Qin X,Zheng R.Serological detection of 2019-nCoV respond to the epidemic:a useful complement to nucleic acid testing[J/OL]. medRxiv 2020.03.04.20030916;doi:https://doi.org/10.1101/2020.03.04.20030916
[65]钟南山院士公布最新研究成果,有两个好消息[EB/OL]. https://www. takefoto. cn/viewnews-2073971. html
[66]中国政府网.关于新冠肺炎治疗药物、疫苗、病毒宿主,有这些最新消息[EB/OL]. http://www.gov.cn/fuwu/2020-02/15/content_5479428.html
[67]Li H,Wang Y M,Xu J Y,Cao B. Potential antiviral therapeutics for 2019 novel coronavirus[J]. Zhonghua Jie He He Hu Xi Za Zhi,2020,43(3):170-172.
[68]Lu H. Drug treatment options for the 2019-new coronavirus(2019-nCoV)[J]. Biosci Trends,2020,14(1):69-71.
[69]Richardson P,Griffin I,Tucker C,Smith D,Oechsle O,Phelan A,Stebbing J. Baricitinib as potential treatment for 2019-nCoV acute respiratory disease[J/OL].Lancet,2020,395(10223):e30-e31.
[70]Wang M,Cao R,Zhang L,Yang X,Liu J,Xu M,Shi Z,Hu Z,Zhong W,Xiao G. Remdesivir and chloroquine effectively inhibit the recently emerged novel coronavirus(2019-nCoV)in vitro[J]. Cell Res,2020,30(3):269-271.
[71]湖北省新型冠状病毒肺炎疫情防控工作指挥部新闻发布会[2020年2月13日][EB/OL]. http://www.chinanews.com/shipin/spfts/20200217/2558.shtml
基本信息:
DOI:10.13242/j.cnki.bingduxuebao.003677
中图分类号:R373.1
引用信息:
[1]谢茜,伍政宇,舒跃龙.2019新型冠状病毒的研究进展[J].病毒学报,2020,36(03):493-501.DOI:10.13242/j.cnki.bingduxuebao.003677.
基金信息:
科技部重点研发计划项目新型冠状病毒感染的肺炎疫情应急(项目号:2020YFC0840900),题目:2019-nCoV基因组变异规律和流行趋势预测研究~~
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