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为了研究ELISA和IFA检测鸡血清中ALV-A/B特异性抗体的相关性,将A/B亚群禽白血病病毒接种到DF1细胞上,用ELISA检测过的鸡血清作为一抗进行IFA检测,比较ELISA检测的S/P值与IFA检测的血清效价之间的相关性。结果表明ELISA检测鸡血清中ALV-A/B抗体的S/P值与IFA检测ALV-A/B的血清效价之间存在显著正相关(r=0.974 35;P﹤0.001)。由此可推测ELISA检测鸡血清的S/P值与IFA检测的血清效价之间存在显著相关性,且呈正相关。IFA检测血清阳性率与ELISA检测结果完全一致,因此当血清样品数量少时,可用IFA检测替代ELISA检测,既节约检测成本也提高了检测的准确性。
Abstract：To study the correlation between ELISA and IFA tests in detection of ALV-A/B antibody in chicken sera,ELSA S/P values and IFA titers for different serum samples were measured and statistically analyzed.The results indicated that there was a strong positive correlation between ELISA S/P values and IFA titers(r=0.97435,P<0.001).Because the positive correlation between ELISA and IFA was so strong and antibody positive rates were identical in two tests,it suggested that IFA could be used as a alternative method to replace ELISA kit when only limited numbers of samples to be tested to reduce the cost and increase the sensitivity.
[1]Saif Y M,Fadly A M,Glison J R,et al.Disease ofpoultry.12th ed[M].Blackwell Publishing Ames,2008:514-568.
[2]朱美真,吴玉宝,崔治中.山东地方品系鸡中一株ALV-A的分离鉴定[J].中国传染病学报,2009,17(4):31-35.
[3]赵冬敏,张青婵,崔治中.芦花鸡中B亚群禽白血病病毒的分离与鉴定[J].病毒学报,2010,26:53-57.
[4]崔治中.禽白血病及其鉴别诊断和预防控制[J].中国家禽,2010.8:1-12.
[5]张青婵,赵冬敏,郭慧君,崔治中.Isolation and identifi-cation of a subgroup a avian leukosis virus from importedmeat-type grand-parent chickens[J].Virologica Sinica,2010,25(2):130-136.
[6]崔治中.免疫抑制性病毒多重感染在鸡群疫病发生和流行中的作用[J].畜牧兽医学报,2003,34(5):417-421.
[7]Xu B,Dong W,Yu C,et al.Occurrence of avian leuko-sis virus subgroup J in commercial layer flocks in China[J].Avian Pathol,2004,33(1):13-17.
[8]成子强,张利,刘思当,等.中国麻鸡中发现禽J亚群白血病[J].微生物学报,2005,45(4):584-587.
[9]徐镔蕊,董卫星,余春明,等.用ALV-J gp85单克隆抗体证明蛋鸡存在J亚群禽白血病[J].畜牧兽医学报,2005,36(3):269-271.
[10]Sun S H,Cui Z.Epidemiological and pathologicalstudies of subgroup J avian leukosis virus infections inChinese local“yellow”chickens[J].Avian Pathology,2007,36(3):221-226.
[11]成子强,刘思当,孟祥凯,等.商品蛋鸡成髓细胞瘤、血管瘤型J亚群白血病病理学初报[J].畜牧兽医学报,2008,39(7):935-940.
[12]武专昌,朱美真,崔治中.二株B亚群禽白血病病毒全基因组序列及其在细胞上的复制性比较[J].病毒学报,2011,27:447-455.
[13]Barbosa T,Zavala G,Cheng S.Molecular character-ization of three recombinant isolates of avian leukosisvirus obtained from contaminated Marek’s diseasevaccines[J].Avian Dis,2008,52:245-252.
[14]Zavala G.and Cheng S.Experimental infection with a-vian leukosis virus isolated from Marek's disease vac-cines[J].Avian Dis,2006,50(2):232-237.
[15]董宣,刘娟,崔治中,等.J亚群禽白血病病毒NX0101株的TCID50与p27抗原之的相关性研究[J].病毒学报,2011,6(27):521-525.
基本信息:
DOI：10.13242/j.cnki.bingduxuebao.002330
中图分类号:S858.31
引用信息:
[1]李薛,李德庆,赵鹏,等.ELISA与IFA检测鸡血清ALV-A/B特异性抗体相关性比较[J].病毒学报,2012,28(06):615-620.DOI:10.13242/j.cnki.bingduxuebao.002330.
基金信息:
国家公益性行业(农业)科研专项种鸡场禽白血病防控与净化技术的集成(201203055)
2012-12-18
2012-12-18
2012-12-18