扬州大学畜牧兽医学院!江苏扬州225009,扬州大学畜牧兽医学院!江苏扬州225009,山东农业大学动物科技学院,山东泰安,扬州大学畜牧兽医学院!江苏扬州225009,Avian Disease and Oncology Lab USDA,East Lansing!MI48823,USA,Avian Disease and Oncology Lab USDA,East Lansing!MI4
通过接种鸡胚成纤维细胞、聚合酶链式反应 (PCR)技术及特异性单抗的间接荧光抗体反应 (IFA) ,从某大型肉用型种鸡场的疑似J亚群白血病的病鸡中 ,以及 2 5个临床健康的商品代肉鸡群的 2群中 ,分离鉴定出J亚群禽白血病病毒 (ALV J)。在用抗ALV Jgp85单克隆抗体JE9的IFA中 ,来自病鸡群的两株病毒SD990 1和SD990 2呈强阳性反应 ,来自临床健康肉鸡群的YZ990 1和YZ990 2株呈弱阳性反应。对YZ990 1株和SD990 2株的PCR产物直接测序结果表明 ,这二个中国株的囊膜蛋白 gp85的氨基酸组成与原型株HPRS 10 3及美国分离株ADOL Hcl有 89%～ 93%的同源性 ,它们相互间的同源性是 92 %。其 3′端LTR区与原型株HPRS 10 3则有 95 %～ 97%的同源性 ,但中国的两株病毒在紧靠 3′端LTR的“E”成分中 ,出现了一个 139个碱基的缺失性突变 ,而代之以一个 11个碱基的插入序列。
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基本信息:
DOI：10.13242/j.cnki.bingduxuebao.001274
中图分类号:S852.65
引用信息:
[1]杜岩,崔治中,秦爱建,RFSilva,LFLee.鸡的J亚群白血病病毒的分离及部分序列比较[J].病毒学报,2000(04):341-346.DOI:10.13242/j.cnki.bingduxuebao.001274.
基金信息:
国家自然科学基金生物学部主任基金!项目资助 (30 0 40 0 11)