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本研究比较了从山东地方品系鸡群分离到的二株B亚型禽白血病病毒(ALV)SDAU09E3和SDAU09C2的全基因组序列及它们在细胞培养上的复制动态。这二株ALV-B的同源性为95.4%,与GenBank中3株B亚群参考株之间的同源性也均在91.0%～94.9%间,而与其它亚群参考株的同源性均低于87.9%。与亚群无关的gag、pol基因和LTR的核苷酸序列比较表明,这二株ALV-Bgp85基因的gag和pol基因与所有比较的参考株的同源性均在93%以上。LTR与其他外源性ALV参考株的LTR间的同源性在72.6%～88.3%范围内,但与E亚群内源性ALV的LTR的同源性只有51.5%。然而,这二个ALV-B的LTR的同源性也只有74.8%,远低于其他基因组部分的同源性,特别是它们的LTR的U3区同源性只有68.8%,二者在二个CAAT分布上也显著不同。对这二株ALV-B在DF-1细胞上的复制动态比较表明,它们在细胞培养上清液中的TCID50值非常类似,但SDAU09E3株核衣壳蛋白p27抗原的含量显著高于SDAU09C2株。这表明,同一亚群的不同毒株在复制过程中,所表达的p27抗原量与所形成的具有传染性的病毒量间没有平行关系。这一差异与LTR-U3区的相关性则有待应用感染性克隆技术来做进一步深入研究。
Abstract：The purpose of this study was to compare the whole genome sequences and replication dynamics in cell cultures of two Avian leukosis viruses of subgroup B(ALV)isolates,SDAU09E3 and SDAU09C2.Comparison of the amino acid sequences indicated that the gp85 identity of these two subgroup B isolates was 95.4%,the identity with other three ALV-B reference strains was 91.0%-94.9%,and less than 87.9% with ALV subgroup A,C,D,E and J.Comparison of the nucleotide sequence of gag and pol genes indicated that homologies of gag gene and pol gene of these two ALV-B isolates with all compared reference strains of different subgroups were above 93%.Homologies of LTR sequence of these two ALV-B isolates with other exogenous ALVs subgroups A,B,C,D and J were 72.6%-88.3%,but only 51.5% when compared with endogenous ALV subgroup E.The identity of LTR between these two ALV-B strains was only 74.8%,which was far lower than the identity of other genes.The identity of U3 region of LTR between these two ALV-B isolates was only 68.8% and there were obvious differences in the number CAAT Boxes.Replication dynamics in DF-1 cell indicated that the value of TCID50 was similar between 2 isolates but the concentration of nucleocapsid protein p27 antigen of SDAU09E3 was significantly higher than SDAU09C2 in cell culture supernatant,which indicated there was no parallel relationship between p27 antigen concentration and infectious virus particles.Whether such difference was resulted from the diversity of U3 region of LTR,further studies with their recombinant infectious clones is necessary.
[1]Weiss R A,Teich N,Varmus H.RNA tumor virus,2nd ed.Supplements and appendices[M].Cold SpringHarbor Laboratory,Cold Spring Harbor,NewYork.1982.
[2]Payne L N,Brown S R,Bumstead K,et al.A novelsubgroup of exogenous avian leukosis virus in chickens.J Gen Virol,1991,72:801-807.
[3]杜岩,崔治中,秦爱建,等.鸡的J亚群白血病病毒的分离及部分序列比较[J].病毒学报,2000,16:341-346.
[4]Cui Z,Du Y,Zhang Z,et al.Comparison of Chinesefield strains of avian leukosis subgroup J viruses withprototype strain HPRS-103and United States strains[J].Avian Dis,2003,47:1321-1330.
[5]张志,崔治中,赵宏坤,等.商品代肉鸡J亚群禽白血病的病理及病毒分离鉴定[J].中国兽医杂志,2002,38:6-8.
[6]Xu B,Dong W,Yu C,et al.Occurrence of avian leuko-sis virus subgroup J in commercial layer flocks in China[J].Avian Pathol,2004,33:13-17.
[7]王辉,崔治中.蛋鸡J亚群白血病病毒的分离鉴定与序列分析[J].病毒学报,2008,24:369-375.
[8]Sun S H,Cui Z Z.Epidemiological and pathologicalstudies of subgroup J avian leukosis virus infectious inChinese coca l“yellow”chicken[J].Avian Pathol,2007,36:221-226.
[9]成子强,张利,刘思当.中国麻鸡中发现禽J亚群白血病[J].微生物学报,2005,45:584-587.
[10]赵冬敏,张青禅,崔治中.芦花鸡中B亚群禽白血病病毒的分离与鉴定[J].病毒学报,2010,26:53-57.
[11]Lucy F.Lee R F.Silva Y Q,Cheng,et al.Character-ization of monoclonal antibodies to avian leukosis viru-ses[J].Avian Dis,1985,30,132-138.
[12]Cullen B R,Raymond K,G Ju.Functional analysis ofthe transcription control region located within the avianretroviral long terminal repeat[J].Mol Cell Bio,1985,5:438-447.
[13]Cullen B R,Raymond K,G Ju.Transcriptional activi-ty of avian retroviral long terminal repeats directly cor-relates with enhancer activity[J].J Virol,1985,53:515-521.
[14]Zachow K R,Conklin K F.CArG,CCAAT,andCCAAT like protein binding sites in avian retroviruslong terminal repeat enhancers[J].J Virol,1992,66:1959-1970.
[15]Bai J,Howes K,Payne L N,Skinner M A.Sequenceof host range determinants in the en6gene of a full-length infectious proviral clone of exogenous avian leu-kosis virus HPRS-103confirms that it represents a newsubgroup(designated J)[J].J Gen Virol,1995,76:181-187.
[16]Fadly A M,Smith E J.Isolation and some characteris-tics of a subgroup J-like avian leukosis virus associatedwith myeloid leukosis in meat-type chickens in the U-nited States[J].Avian Dis,1999,43:391-400.
[17]Zavala G,Cheng S.Detection and characterization of a-vian leukosis virus in Marek's disease vaccines[J].Avi-an Dis,2006,50:209-215.
[18]Silva R F,Fadly A M,Taylor S P.Development of apolymerase chain reaction to differentiate avain leukosisvirus(ALV)subgroup:detection of an ALV contami-nant in commercial Marek's disease vaccines[J].AvianDis,2007,51:663-667.
[19]Barbosa T,Zavala G,Cheng S.Molecular character-ization of three recombinant isolates of avian leukosisvirus obtained from contaminated Marek's disease vac-cines[J].Avian Dis,2008,52:245-252.
[20]Zavala G,Cheng S.Experimental infection with avianleukosis virus isolated from Marek's disease vaccines[J].Avian Dis,2006,50(2):232-237.
[21]崔治中.禽白血病及其鉴别诊断和预防控制[J].中国家禽,2010.8:1-12.
基本信息:
DOI：10.13242/j.cnki.bingduxuebao.002204
中图分类号:S852.65
引用信息:
[1]武专昌,朱美真,边晓明,等.二株B亚群禽白血病病毒全基因组序列及其在细胞上的复制性比较[J].病毒学报,2011,27(05):447-455.DOI:10.13242/j.cnki.bingduxuebao.002204.
基金信息:
禽白血病流行病学和防控措施的示范性研究(200803019)
2011-10-09
2011-10-09
2011-10-09