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评估一种快速和敏感富集草莓中诺如病毒的方法,为病毒性食源性疾病的防控提供支持。以鼠诺如病毒-1(MNV-1)为模式病毒,考察Tralk洗脱液、TGBE洗脱液和NaOH洗脱液对MNV-1的洗脱效果,检测5%、10%和15%PEG6000/0.3M NaCl对MNV-1的富集效果,评估优化后方法对草莓中诺如病毒的富集效果和超滤二次浓缩对诺如病毒富集效果影响。结果表明TGBE缓冲液洗脱效果优于Tralk洗脱液和NaOH洗脱液;10%PEG6000/0.3M NaCl与15%PEG6000/0.3M NaCl对MNV-1的富集效果相同,且二者的富集效果均高于5%PEG6000/0.3M NaCl;该优化方法可在7h内完成草莓中诺如病毒的富集,诺如病毒的回收率最高可达44.04%,超滤二次浓缩后,诺如病毒的检测限达到102基因拷贝数/10g草莓。
Abstract：Several norovirus(NoVs)outbreaks caused by the consumption of strawberry have been reported during recent years.In this study,a rapid and sensitive method for concentrating NoVs from strawberry has been evaluated.Murine norovirus-1(MNV-1)was used as an alternative virus for norovirus.Tralk,TGBE and NaOH were used for elution of MNV-1.5% ,10% and 15% PEG6000/0.3 M NaCl were used to assess the enrichment effect of MNV-1.Results showed that TGBE buffer,10% PEG6000/0.3 M NaCl and 15% PEG6000/0.3 M NaCl were effective for virus elution and concentration.The recovery rate of norovirus is 3.53% ~44.04% in strawberry.Using ultrafiltration as the second concentration,the minimum test limit was 102 genocopy/10 g of fresh strawberry samples.The results demonstrated that the optimized method could be used in the determination of NoVs from fresh strawberry samples with good sensitivity and efficiency.
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基本信息:
DOI：10.13242/j.cnki.bingduxuebao.003308
中图分类号:R155.5
引用信息:
[1]王飞,李慧莹,靳淼,等.草莓中诺如病毒不同洗脱和富集方法的评估[J].病毒学报,2018,34(01):94-100.DOI:10.13242/j.cnki.bingduxuebao.003308.
基金信息:
国家自然科学基金资助项目(项目号:81601813),题目:P[6]基因型轮状病毒跨物种传播的分子机制研究~~
2018-01-24
2018-01-24
2018-01-24