病毒学报

本研究基于实验室前期筛选鉴定出的牛结节性皮肤病毒L1R、A27L、A33R和P32上的4个优势抗原肽等量混合作为包被抗原建立了牛结节性皮肤病病毒间接ELISA抗体检测方法，并对其敏感性、特异性、重复性进行验证。结果显示，该方法灵敏度可检测到1∶640稀释的阳性血清；对健康牛血清和其他病毒阳性血清的检测结果为阴性，特异性100%；批内变异系数在2.3%～11.9%，批间变异系数在3.4%～12.6%。和进口试剂盒对免疫不同时间点的牛血清进行对比检测，结果显示本研究的ELISA方法灵敏度高于进口试剂盒。综上结果表明该检测方法具有敏感性高、特异性好、重复性好等优势，可用于牛结节性皮肤病抗体检测。

This study established an indirect ELISA for the detection of antibodies against lumpy skin disease virus(LSDV) using a combination of four dominant antigenic peptides from LSDV proteins L1R, A27L, A33R and P32 as the coating antigens, which were identified through prior laboratory screening. The sensitivity, specificity, and reproducibility of the method were validated. The results showed that t this assay could detect antibodies at a dilution of 1:640. The test yielded negative results for sera from healthy cattle and those positive for other viruses,indicating 100% specificity. The intra-assay coefficient of variation ranged from 2.3% to 11.9%, while the inter-assay coefficient of variation ranged from 3.4% to 12.6%. A comparison with an imported ELISA kit using sera collected at different time points post-immunization demonstrated that the ELISA developed in this study exhibited higher sensitivity. These findings suggest that the established ELISA method offers high sensitivity, strong specificity, and excellent reproducibility, making it a valuable tool for LSDV antibody detection.