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柯萨奇病毒A14型属于肠道病毒A组，可引起手足口病和无菌性脑膜炎等疾病，但目前全球对CVA14的分子流行病学研究较少，尚无对CVA14基于全长VP1区进行明确分型的相关研究。因得到中国手足口病监测网络的技术支持，本研究获得2009-2019年在中国大陆分离到的15株CVA14，应用RT-PCR对其全长VP1区进行扩增、测序和分析，并与GenBank中下载的22条CVA14全长VP1序列共同构建系统发育树。结果显示，2009-2019年中国大陆分离的15株CVA14与原型株G-14的核苷酸与氨基酸相似性分别为81.8%～82.9%与95.6%～96.9%,15株CVA14之间的核苷酸与氨基酸相似性分别为91.7%～99.7%与98.3%～100%。中国大陆所有的22条CVA14序列之间的核苷酸与氨基酸相似性分别为91.7%～100.0%与98.3%～100.0%。中国大陆所有的CVA14与国外8条CVA14序列之间的核苷酸与氨基酸相似性分别为80.7%～86.0%与94.9%～97.9%。基于全长VP1系统发育树，全球CVA14可划分为A-G七个基因型，目前全球范围内流行的CVA14的基因型主要为G基因型，中国大陆流行的基因型全部为G基因型。本研究建立了全球CVA14基于全长VP1的基因分型方法，揭示了CVA14在中国大陆及全球范围内的分子流行特征，为CVA14的致病机制研究提供了分子流行病学方面的基础。
Abstract：Coxsackievirus A14 belongs to Enterovirus group A, which cause hand-foot-mouth disease and aseptic meningitis. However, there are few molecular epidemiological studies on CVA14 in the world, and there is no relevant study on the clear typing of CVA14 based on the full-length VP1 region. With the technical support of China HFMD Surveillance Network, 15 CVA14 strains isolated in Chinese mainland from 2009 to 2019 were obtained in this study. The full-length VP1 region of 15 CVA14 strains was amplified by RT-PCR, sequenced and analyzed, and the phylogenetic tree was constructed together with 22 full-length VP1 sequences downloaded from GenBank. Results showed that the nucleotide and amino acid similarities between the 15isolated strains of CVA14 from 2009 to 2019 and the prototype strain G-14 were 81.8%～82.9% and 95.6%～96.9%, respectively. The nucleotide and amino acid similarities among the 15 strains of CVA14 were 91.7%～99.7% and 98.3%～100%, respectively. The nucleotide and amino acid similarities among all 22 CVA14sequences in Chinese mainland were 91.7%～100.0% and 98.3%～100.0%, respectively. The nucleotide and amino acid similarities between all CVA14 sequences in Chinese mainland and eight foreign CVA14 sequences were 80.7%-86.0% and 94.9%-97.9%, respectively. Based on full-length VP1 phylogenetic tree, global CVA14 strains could be divided into seven genotypes: A-G. Currently, most of the CVA14 genotypes prevalent in the world were G genotype, and all the genotypes prevalent in Chinese mainland were G genotype.In this study, we established a full-length VP1 genotyping method for global CVA14, revealed the molecular epidemic characteristics of CVA14 in Chinese mainland and all over the world, and provided a molecular epidemiological basis for study of the pathogenesis of CVA14.
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基本信息:
DOI：10.13242/j.cnki.bingduxuebao.004377
中图分类号:S852.65
引用信息:
[1]于力恒,郭琴,卫海燕,等.2009-2019年中国大陆柯萨奇病毒A14型基因特征分析[J].病毒学报,2023,39(06):1570-1577.DOI:10.13242/j.cnki.bingduxuebao.004377.
基金信息:
国家重点研发计划（项目号：2021YFF0703801）,题目：微生物科学数据整合标准及共享服务平台建设;国家重点研发计划（项目号：2021YFC2302003）,题目：病毒监测网络数据标准及数据平台建设; 北京市自然科学基金（项目号：L192014）,题目：EVA71疫苗大规模应用后对我国手足口病病原谱的影响及其保护效果评价研究~~
2023-06-05
2023
2023-08-03
2023-08-21
2023
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2023-08-22
2023-08-22
2023-08-22