山东农业大学动物医学院;
为探明我国地方品种鸡群禽白血病病毒(Avian leukosis virus,ALV)的特点,通过接种DF-1细胞及细胞培养上清液p27抗原的检测,从芦花鸡中分离得到三株外源性ALV禽白血病病毒,分别是JS11C1、JS11C2和JS11C3,并对其进行亚群鉴定分析。用PCR方法扩增env基因测序,并与已知鸡源各亚群ALV的囊膜蛋白(gp85)作氨基酸同源性比较。这三株ALV的env基因的gp85大小为1 005bp,编码335个氨基酸;env基因的gp37大小为609bp,编码203个氨基酸。三个毒株之间gp85的同源性为91.9%～97.0%。与A、B、C、D和E五个经典亚群在GenBank中已发表的18个毒株的gp85的同源性仅在77.7%～84.6%间,显著低于鸡群中常见的A、B、E各亚群内的同源性范围(分别为88.2%～98.5%,91.6%～98.8%和97.9%～99.4%),而与J亚群参考株的同源性更是只有34.2%～36.5%。上述结果表明,芦花鸡分离到的三株病毒可能是不同于鸡源ALV已知6个亚群的一个新亚群,按国际上对ALV亚群分类的习惯,初步将其定名为K亚群。
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[2]Payne L N,Brown S R,Bumstead K,et al.A novelsubgroup of exogenous avian leukosis virus in chickens[J].J Gen Virol,1991,72:801-807.
[3]Payne L N,Howes K,Gillespie A M,et al.Host rangeof Rous sarcoma virus pseudo type RSV(HPRS-103)in12avian species:support for a new avian retrovirusenvelope subgroup designated J[J].J Gen Virol,1992,73:2995-2997.
[4]杜岩,崔治中,秦爱建,等.鸡的J亚群白血病病毒的分离及部分序列比较[J].病毒学报,2000,16:341-346.
[5]张志,崔治中,赵宏坤,等.商品代肉鸡J亚群禽白血病的病理及病毒分离鉴定[J].中国兽医杂志,2002,38:6-8.
[6]Cui Z,Du Y,Zhang Z,et al.Comparison of Chinesefield strains of avian leukosis subgroup J viruses withprototype strain HPRS-103and United States strains[J].Avian Dis,2003,47:1321-1330.
[7]ZHANG Qing-chan,ZHAO Dong-min,GUO Hui-jun,et al.Isolation and identification of a subgroup a avianleukosis virus from imported meat-type grand-parentchickens[J].Virol Sin,April,2010,25(2):130-136.
[8]Xu B,Dong W,Yu C,et al.Occurrence of avian leuko-sis virus subgroup J in commercial layer flocks in China[J].Avian Pathol,2004,33:13-17.
[9]王辉,崔治中.蛋鸡J亚群白血病病毒的分离鉴定与序列分析[J].病毒学报,2008,24:369-375.
[10]Sun S H,Cui Z Z.Epidemiological and pathologicalstudies of subgroup J avian leukosis virus infectious inchinese local“yellow”chicken[J].Avian Pathol,2007,36:221-226.
[11]成子强,张利,刘思当,等.中国麻鸡中发现禽J亚群白血病[J].微生物学报,2005,45:584-587.
[12]刘绍琼,王波,张振杰,等.817肉杂鸡肉瘤组织分离出A、J亚禽白血病病毒[J].畜牧兽医学报,2011,42(3):396-401.
[13]倪伟,秦立廷,孙洪磊,等.某海兰褐鸡场祖代、父母代及商品代鸡血管瘤型ALV-J的分离与鉴定[J].畜牧兽医学报,2011,42(9):1341-1345.
[14]张振杰,刘少琼,王波,等.地方品种皖南黄肉种鸡ALV-J与REV的共感染及其分子变异分析[J].中国农业科学,2011,44(11):2379-2386.
[15]朱美珍,吴玉宝,崔治中.山东地方品系鸡中一株A亚群鸡的白血病病毒的分离和鉴定[J].中国动物传染病学报,2009,17(4):31-35.
[16]赵冬敏,张青禅,崔治中.芦花鸡中B亚群禽白血病病毒的分离与鉴定[J].病毒学报,2010,26(1):53-57.
[17]武专昌,朱美珍,边小明,等.两株B亚群禽白血病病毒全基因测序及其在细胞上的复制性比较[J].病毒学报,2011,27(5):447-455.
[18]Fadly A M,Smith E J.Isolation and some characteris-tics of a subgroup J-like avian leukosis virus associatedwith myeloid leukosis in meat-type chickens in theUnited States[J].Avian Dis,1999,43:391-400.
[19]Silva R F,Fadly A M,Taylor S P.Development of apolymerase chain reaction to differentiate avain leukosisvirus(ALV)subgroup:detection of an ALV contami-nant in commercial Marek's disease vaccines[J].AvainDis,2007,51:663-667.
[20]Zavala G,Cheng S.Detection and characterization ofavian leukosis virus in Marek's disease vaccines[J].Avian Dis,2006,50:209-215.
[21]Payne L N,Brown S R,Bumstead N,et al.A novelsubgroup of exogenous avian leukosis virus in chickens[J].J Gen Virol,1991,72:801-807.
[22]崔治中.鸡白血病及其鉴别诊断和预防调控[C].鸡淋巴白血病防控技术研讨会论文集.2010.
基本信息:
DOI：10.13242/j.cnki.bingduxuebao.002329
中图分类号:S852.65
引用信息:
[1]王鑫,赵鹏,崔治中.我国地方品种鸡分离到的一个禽白血病病毒新亚群的鉴定[J].病毒学报,2012,28(06):609-614.DOI:10.13242/j.cnki.bingduxuebao.002329.
基金信息:
公益性行业(农业)科研专项(#201203055)