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为探明我国地方品种鸡群禽白血病病毒(Avian leukosis virus,ALV)的特点,通过接种DF-1细胞及细胞培养上清液p27抗原的检测,从芦花鸡中分离得到三株外源性ALV禽白血病病毒,分别是JS11C1、JS11C2和JS11C3,并对其进行亚群鉴定分析。用PCR方法扩增env基因测序,并与已知鸡源各亚群ALV的囊膜蛋白(gp85)作氨基酸同源性比较。这三株ALV的env基因的gp85大小为1 005bp,编码335个氨基酸;env基因的gp37大小为609bp,编码203个氨基酸。三个毒株之间gp85的同源性为91.9%～97.0%。与A、B、C、D和E五个经典亚群在GenBank中已发表的18个毒株的gp85的同源性仅在77.7%～84.6%间,显著低于鸡群中常见的A、B、E各亚群内的同源性范围(分别为88.2%～98.5%,91.6%～98.8%和97.9%～99.4%),而与J亚群参考株的同源性更是只有34.2%～36.5%。上述结果表明,芦花鸡分离到的三株病毒可能是不同于鸡源ALV已知6个亚群的一个新亚群,按国际上对ALV亚群分类的习惯,初步将其定名为K亚群。
Abstract：In order to clarify Avian leukosis virus(ALV) characteristics from Chinese native chicken breeds,three ALV JS11C1,JS11C2 and JS11C3 were isolated from Chinese native breed "luhua" by inoculation of DF1 cell culture and detection of p27 antigen.Using PCR amplification of env gene,the amplified gp85 genes were analyzed and compared to all six chicken ALV subgroups reported.The gp85 genes of these three viruses were 1 005bp in length and encoded 335 amino acids,and the gp37 genes were 609bp and encoded 203 amino acids.The homology of gp85 among these three isolated strains was 91.9%-97.0%.Comparing to 18 stains of subgroup A,B,C,D,E published in GenBank,the homology was only in the range of 77.7%-84.6%,significantly lower than the gp85 homology observed within the common chicken subgroups A(88.2%-98.5%),B(91.6%-98.8%),and E(97.9%-99.4%).The gp85 homology compared with subgroup J was only 34.2%-36.5%.These results suggested that three isolated strains from Chinese native breed "luhua" belong to a new subgroup different from all six known subgroups from Chickens,and thus designated as subgroup K.
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基本信息:
DOI：10.13242/j.cnki.bingduxuebao.002329
中图分类号:S852.65
引用信息:
[1]王鑫,赵鹏,崔治中.我国地方品种鸡分离到的一个禽白血病病毒新亚群的鉴定[J].病毒学报,2012,28(06):609-614.DOI:10.13242/j.cnki.bingduxuebao.002329.
基金信息:
公益性行业(农业)科研专项(#201203055)