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阐明人乳头瘤病毒（Human papilloma virus,HPV）感染的女性阴道局部免疫及阴道微生物菌群的情况，并探讨其与HPV感染的相关性。本研究收集2017年10月至2018年3月于我院初诊为HPV阳性的175名女性，根据其宫颈细胞学结果分为4组，分别为无上皮内病变或恶性病变（No intraepithelial lesions or malignant lesions,NILM）、意义未明的不典型鳞状细胞（Atypical squamous cells of undetermined significance,ASCUS）、低级别鳞状上皮内病变（Low-grade squamous intraepithelial lesion,LSIL）、高级别鳞状上皮内病变（High-grade squamous intraepithelial lesion,HSIL），将同期体检为NILM/HPV（-）的62名健康女性作为对照组。在显微镜下检测患者的阴道微生物（细菌、真菌等）、滴虫，采用酶联免疫法检测阴道局部IL-2、IL-12、IFN-γ、sIgA的表达量。结果显示：与对照组相比，HPV（+）组阴道清洁程度Ⅲ°～Ⅳ°概率增高（P<0.05）；细菌性阴道病（Bacterial vaginosis,BV）发病率增高（P<0.05）；真菌感染率增高（P<0.05）;IL-2、IL-12、sIgA在阴道局部的表达量降低（P<0.05）;IL-2、sIgA随着宫颈病变的加重，在阴道局部的表达量逐渐降低（P<0.05）。本研究揭示，HPV感染可改变阴道局部的免疫应答及阴道微生物菌种，为HPV感染后的阴道局部治疗及预防HPV感染提供了依据。
Abstract：The aim of this study was to clarify the relationships between vaginal local immunity and microbiota in women infected by human papilloma virus(HPV),and to explore the correlations with HPV infection. A total of 175 patients who were initially diagnosed as HPV-positive at the Third Affiliated Hospital of Zhengzhou University from October 2017 to March 2018 were divided into four groups:no intraepithelial lesions or malignant lesions(NILM),atypical squamous cells of undetermined significance(ASCUS),low-grade squamous intraepithelial lesions(LSIL),and high-grade squamous intraepithelial lesions(HSIL). Sixty-two NILM/HPV-negative patients were included as the control group. Vaginal microbes(bacteria, fungi,Trichomonas,and so on)and the expression of interleukin(IL)-2,IL-12,interferon(IFN)-γ,and secretory immunoglobulin A(sIgA)were determined in all patients. Compared with the control[HPV（-）]group,the probability of vaginal cleanliness degree Ⅲ° ～Ⅳ° in the HPV(+)group was higher(P < 0.05),and the probability of being with bacterial vaginosis or being fungus(+)was increased(P < 0.05). The expression of IL-2,IL-12,and sIgA in the HPV(+)groups was significantly lower than that in the control group(P <0.05). The expression of IL-2 and sIgA decreased gradually with the aggravation of cervical lesions(P <0.05). Imbalance of the microbiota in the vagina can accelerate,and affect the outcome of,HPV infection. HPV infection can change the local vaginal immune response and vaginal microbial species. These findings provide a basis for local vaginal treatment after HPV infection and prevention of HPV infection.
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基本信息:
DOI：10.13242/j.cnki.bingduxuebao.004093
中图分类号:R737.33
引用信息:
[1]刘菲,李肖甫,王蕊杰,等.阴道IL-2、IL-12、IFN-γ、sIgA的表达及微生物与HPV感染的关系[J].病毒学报,2022,38(02):348-353.DOI:10.13242/j.cnki.bingduxuebao.004093.
基金信息:
河南省科技厅重点研发与推广专项(项目号：182102310141)，题目：LncRNA启动子区甲基化在宫颈癌发生过程中作用的研究~~
2022-01-06
2022-01-06
2022-01-06